The role of neutrophils during mild and severe influenza virus infections of mice.

Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6(high) neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Michelle D Tate, Lisa J Ioannidis, Ben Croker, Lorena E Brown, Andrew G Brooks, Patrick C Reading
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb7a10a8760b4565ad237b5fafa7db1b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fb7a10a8760b4565ad237b5fafa7db1b
record_format	dspace
spelling	oai:doaj.org-article:fb7a10a8760b4565ad237b5fafa7db1b2021-11-18T06:57:20ZThe role of neutrophils during mild and severe influenza virus infections of mice.1932-620310.1371/journal.pone.0017618https://doaj.org/article/fb7a10a8760b4565ad237b5fafa7db1b2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21423798/?tool=EBIhttps://doaj.org/toc/1932-6203Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6(high) neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of neutrophils in mice that has been shown to bind and deplete additional leukocyte subsets. Using mAb 1A8, we confirm the beneficial role of neutrophils in mice infected with virus strains of intermediate (HKx31; H3N2) or high (PR8; H1N1) virulence whereas treatment of mice infected with an avirulent strain (BJx109; H3N2) did not affect disease or virus replication. Treatment of BJx109-infected mice with mAb RB6-8C5 was, however, associated with significant weight loss and enhanced virus replication indicating that other Gr-1(+) cells, not neutrophils, limit disease severity during mild influenza infections.Michelle D TateLisa J IoannidisBen CrokerLorena E BrownAndrew G BrooksPatrick C ReadingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17618 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michelle D Tate Lisa J Ioannidis Ben Croker Lorena E Brown Andrew G Brooks Patrick C Reading The role of neutrophils during mild and severe influenza virus infections of mice.
description	Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6(high) neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of neutrophils in mice that has been shown to bind and deplete additional leukocyte subsets. Using mAb 1A8, we confirm the beneficial role of neutrophils in mice infected with virus strains of intermediate (HKx31; H3N2) or high (PR8; H1N1) virulence whereas treatment of mice infected with an avirulent strain (BJx109; H3N2) did not affect disease or virus replication. Treatment of BJx109-infected mice with mAb RB6-8C5 was, however, associated with significant weight loss and enhanced virus replication indicating that other Gr-1(+) cells, not neutrophils, limit disease severity during mild influenza infections.
format	article
author	Michelle D Tate Lisa J Ioannidis Ben Croker Lorena E Brown Andrew G Brooks Patrick C Reading
author_facet	Michelle D Tate Lisa J Ioannidis Ben Croker Lorena E Brown Andrew G Brooks Patrick C Reading
author_sort	Michelle D Tate
title	The role of neutrophils during mild and severe influenza virus infections of mice.
title_short	The role of neutrophils during mild and severe influenza virus infections of mice.
title_full	The role of neutrophils during mild and severe influenza virus infections of mice.
title_fullStr	The role of neutrophils during mild and severe influenza virus infections of mice.
title_full_unstemmed	The role of neutrophils during mild and severe influenza virus infections of mice.
title_sort	role of neutrophils during mild and severe influenza virus infections of mice.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/fb7a10a8760b4565ad237b5fafa7db1b
work_keys_str_mv	AT michelledtate theroleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT lisajioannidis theroleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT bencroker theroleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT lorenaebrown theroleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT andrewgbrooks theroleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT patrickcreading theroleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT michelledtate roleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT lisajioannidis roleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT bencroker roleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT lorenaebrown roleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT andrewgbrooks roleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice AT patrickcreading roleofneutrophilsduringmildandsevereinfluenzavirusinfectionsofmice
_version_	1718424206656405504

The role of neutrophils during mild and severe influenza virus infections of mice.

Ejemplares similares