Comparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.

Relative expression levels of immune- and non-immune-related mRNAs in chicken intestinal intraepithelial lymphocytes experimentally infected with Eimeria acervulina, E. maxima, or E. tenella were measured using a 10K cDNA microarray. Based on a cutoff of >2.0-fold differential expression compared...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Duk Kyung Kim, Hyun Lillehoj, Wongi Min, Chul Hong Kim, Myeong Seon Park, Yeong Ho Hong, Erik P Lillehoj
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb7ab7430ce249a1baacb8d3e8bdfc99
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fb7ab7430ce249a1baacb8d3e8bdfc99
record_format	dspace
spelling	oai:doaj.org-article:fb7ab7430ce249a1baacb8d3e8bdfc992021-11-18T07:33:33ZComparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.1932-620310.1371/journal.pone.0027712https://doaj.org/article/fb7ab7430ce249a1baacb8d3e8bdfc992011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22140460/?tool=EBIhttps://doaj.org/toc/1932-6203Relative expression levels of immune- and non-immune-related mRNAs in chicken intestinal intraepithelial lymphocytes experimentally infected with Eimeria acervulina, E. maxima, or E. tenella were measured using a 10K cDNA microarray. Based on a cutoff of >2.0-fold differential expression compared with uninfected controls, relatively equal numbers of transcripts were altered by the three Eimeria infections at 1, 2, and 3 days post-primary infection. By contrast, E. tenella elicited the greatest number of altered transcripts at 4, 5, and 6 days post-primary infection, and at all time points following secondary infection. When analyzed on the basis of up- or down-regulated transcript levels over the entire 6 day infection periods, approximately equal numbers of up-regulated transcripts were detected following E. tenella primary (1,469) and secondary (1,459) infections, with a greater number of down-regulated mRNAs following secondary (1,063) vs. primary (890) infection. On the contrary, relatively few mRNA were modulated following primary infection with E. acervulina (35 up, 160 down) or E. maxima (65 up, 148 down) compared with secondary infection (E. acervulina, 1,142 up, 1,289 down; E. maxima, 368 up, 1,349 down). With all three coccidia, biological pathway analysis identified the altered transcripts as belonging to the categories of "Disease and Disorder" and "Physiological System Development and Function". Sixteen intracellular signaling pathways were identified from the differentially expressed transcripts following Eimeria infection, with the greatest significance observed following E. acervulina infection. Taken together, this new information will expand our understanding of host-pathogen interactions in avian coccidiosis and contribute to the development of novel disease control strategies.Duk Kyung KimHyun LillehojWongi MinChul Hong KimMyeong Seon ParkYeong Ho HongErik P LillehojPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27712 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Duk Kyung Kim Hyun Lillehoj Wongi Min Chul Hong Kim Myeong Seon Park Yeong Ho Hong Erik P Lillehoj Comparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.
description	Relative expression levels of immune- and non-immune-related mRNAs in chicken intestinal intraepithelial lymphocytes experimentally infected with Eimeria acervulina, E. maxima, or E. tenella were measured using a 10K cDNA microarray. Based on a cutoff of >2.0-fold differential expression compared with uninfected controls, relatively equal numbers of transcripts were altered by the three Eimeria infections at 1, 2, and 3 days post-primary infection. By contrast, E. tenella elicited the greatest number of altered transcripts at 4, 5, and 6 days post-primary infection, and at all time points following secondary infection. When analyzed on the basis of up- or down-regulated transcript levels over the entire 6 day infection periods, approximately equal numbers of up-regulated transcripts were detected following E. tenella primary (1,469) and secondary (1,459) infections, with a greater number of down-regulated mRNAs following secondary (1,063) vs. primary (890) infection. On the contrary, relatively few mRNA were modulated following primary infection with E. acervulina (35 up, 160 down) or E. maxima (65 up, 148 down) compared with secondary infection (E. acervulina, 1,142 up, 1,289 down; E. maxima, 368 up, 1,349 down). With all three coccidia, biological pathway analysis identified the altered transcripts as belonging to the categories of "Disease and Disorder" and "Physiological System Development and Function". Sixteen intracellular signaling pathways were identified from the differentially expressed transcripts following Eimeria infection, with the greatest significance observed following E. acervulina infection. Taken together, this new information will expand our understanding of host-pathogen interactions in avian coccidiosis and contribute to the development of novel disease control strategies.
format	article
author	Duk Kyung Kim Hyun Lillehoj Wongi Min Chul Hong Kim Myeong Seon Park Yeong Ho Hong Erik P Lillehoj
author_facet	Duk Kyung Kim Hyun Lillehoj Wongi Min Chul Hong Kim Myeong Seon Park Yeong Ho Hong Erik P Lillehoj
author_sort	Duk Kyung Kim
title	Comparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.
title_short	Comparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.
title_full	Comparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.
title_fullStr	Comparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.
title_full_unstemmed	Comparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.
title_sort	comparative microarray analysis of intestinal lymphocytes following eimeria acervulina, e. maxima, or e. tenella infection in the chicken.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/fb7ab7430ce249a1baacb8d3e8bdfc99
work_keys_str_mv	AT dukkyungkim comparativemicroarrayanalysisofintestinallymphocytesfollowingeimeriaacervulinaemaximaoretenellainfectioninthechicken AT hyunlillehoj comparativemicroarrayanalysisofintestinallymphocytesfollowingeimeriaacervulinaemaximaoretenellainfectioninthechicken AT wongimin comparativemicroarrayanalysisofintestinallymphocytesfollowingeimeriaacervulinaemaximaoretenellainfectioninthechicken AT chulhongkim comparativemicroarrayanalysisofintestinallymphocytesfollowingeimeriaacervulinaemaximaoretenellainfectioninthechicken AT myeongseonpark comparativemicroarrayanalysisofintestinallymphocytesfollowingeimeriaacervulinaemaximaoretenellainfectioninthechicken AT yeonghohong comparativemicroarrayanalysisofintestinallymphocytesfollowingeimeriaacervulinaemaximaoretenellainfectioninthechicken AT erikplillehoj comparativemicroarrayanalysisofintestinallymphocytesfollowingeimeriaacervulinaemaximaoretenellainfectioninthechicken
_version_	1718423325092347904

Comparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.

Ejemplares similares