Molecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments

Abstract Despite the fact that natural enemies can synergistically contribute to herbivore pest suppression, sometimes predators engage in intraguild predation (IGP) that might dampen trophic cascades. DNA-based gut-content analysis has become common in assessing trophic connections and biocontrol p...

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Autores principales:	Gabriele Rondoni, Saleh Fenjan, Valeria Bertoldi, Fulvio Ielo, Khaled Djelouah, Chiaraluce Moretti, Roberto Buonaurio, Carlo Ricci, Eric Conti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb83710b6bbf40f79f99ef93d1a466bc
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spelling	oai:doaj.org-article:fb83710b6bbf40f79f99ef93d1a466bc2021-12-02T11:41:04ZMolecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments10.1038/s41598-018-20830-22045-2322https://doaj.org/article/fb83710b6bbf40f79f99ef93d1a466bc2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20830-2https://doaj.org/toc/2045-2322Abstract Despite the fact that natural enemies can synergistically contribute to herbivore pest suppression, sometimes predators engage in intraguild predation (IGP) that might dampen trophic cascades. DNA-based gut-content analysis has become common in assessing trophic connections and biocontrol potential by predators in field systems. Here, we developed a molecular technique that can be used to unravel predation among two ladybirds, Coccinella septempunctata and Hippodamia variegata, and their shared prey, Aphis gossypii. Both ladybirds may provide effective control of the pest. Therefore, understanding their likelihood to engage in IGP is crucial for conservation biological control. Ladybird specimens were collected in melon crop. DNA extraction, primer design and evaluation were conducted. Detectability of prey DNA did not differ significantly between the two ladybirds. H. variegata exhibited higher predation on A. gossypii than C. septempunctata (90.6% vs. 70.9%) and data correction based on DNA detectability confirmed this ranking. IGP was similar among the two species, although corrected data might suggest a stronger predation by C. septempunctata. Intriguingly, IGP by C. septempunctata was lower than predicted by laboratory bioassays, possibly due to the high complexity that arises under field conditions. Implications of our results for biological control and perspectives for ecological network analysis are discussed.Gabriele RondoniSaleh FenjanValeria BertoldiFulvio IeloKhaled DjelouahChiaraluce MorettiRoberto BuonaurioCarlo RicciEric ContiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gabriele Rondoni Saleh Fenjan Valeria Bertoldi Fulvio Ielo Khaled Djelouah Chiaraluce Moretti Roberto Buonaurio Carlo Ricci Eric Conti Molecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments
description	Abstract Despite the fact that natural enemies can synergistically contribute to herbivore pest suppression, sometimes predators engage in intraguild predation (IGP) that might dampen trophic cascades. DNA-based gut-content analysis has become common in assessing trophic connections and biocontrol potential by predators in field systems. Here, we developed a molecular technique that can be used to unravel predation among two ladybirds, Coccinella septempunctata and Hippodamia variegata, and their shared prey, Aphis gossypii. Both ladybirds may provide effective control of the pest. Therefore, understanding their likelihood to engage in IGP is crucial for conservation biological control. Ladybird specimens were collected in melon crop. DNA extraction, primer design and evaluation were conducted. Detectability of prey DNA did not differ significantly between the two ladybirds. H. variegata exhibited higher predation on A. gossypii than C. septempunctata (90.6% vs. 70.9%) and data correction based on DNA detectability confirmed this ranking. IGP was similar among the two species, although corrected data might suggest a stronger predation by C. septempunctata. Intriguingly, IGP by C. septempunctata was lower than predicted by laboratory bioassays, possibly due to the high complexity that arises under field conditions. Implications of our results for biological control and perspectives for ecological network analysis are discussed.
format	article
author	Gabriele Rondoni Saleh Fenjan Valeria Bertoldi Fulvio Ielo Khaled Djelouah Chiaraluce Moretti Roberto Buonaurio Carlo Ricci Eric Conti
author_facet	Gabriele Rondoni Saleh Fenjan Valeria Bertoldi Fulvio Ielo Khaled Djelouah Chiaraluce Moretti Roberto Buonaurio Carlo Ricci Eric Conti
author_sort	Gabriele Rondoni
title	Molecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments
title_short	Molecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments
title_full	Molecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments
title_fullStr	Molecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments
title_full_unstemmed	Molecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments
title_sort	molecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fb83710b6bbf40f79f99ef93d1a466bc
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Molecular detection of field predation among larvae of two ladybird beetles is partially predicted from laboratory experiments

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