Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV

African swine fever virus (ASFV) is an important viral pathogen infecting pigs worldwide throughout the pig industry. CD2v (an outer-membrane glycosylated protein of ASFV)-unexpressed lower-virulence mutants have appeared in China and other countries in recent years. Using OIE-recommended quantitati...

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Autores principales:	Changjie Lv, Ya Zhao, Lili Jiang, Li Zhao, Chao Wu, Xianfeng Hui, Xiaotong Hu, Ziqi Shao, Xiaohan Xia, Xiaomei Sun, Qiang Zhang, Meilin Jin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	African swine fever virus (ASFV) ELISA differentiation non-hemadsorption lower-virulence natural mutants Science Q
Acceso en línea:	https://doaj.org/article/fb8b0ee8709d444b93c0be364b88f0cf
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spelling	oai:doaj.org-article:fb8b0ee8709d444b93c0be364b88f0cf2021-11-25T18:11:16ZDevelopment of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV10.3390/life111112142075-1729https://doaj.org/article/fb8b0ee8709d444b93c0be364b88f0cf2021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1214https://doaj.org/toc/2075-1729African swine fever virus (ASFV) is an important viral pathogen infecting pigs worldwide throughout the pig industry. CD2v (an outer-membrane glycosylated protein of ASFV)-unexpressed lower-virulence mutants have appeared in China and other countries in recent years. Using OIE-recommended quantitative PCR and ELISA methods, people can accurately judge whether pigs are infected with wild-type ASFV. However, the strategy has failed to distinguish ΔCD2v lower-virulence mutants and wild-type ASFV infection. Here, we expressed and purified the CD2v and p30 proteins via CHO cells and successfully established a dual enzyme-linked immunosorbent assay (ELISA), which can be used to differentiate pigs infected with wild-type ASFV or with CD2v-unexpressed lower-virulence mutants. The dual ELISA showed excellent specificity without cross-reactions with antibodies of PRRSV, CSFV, JEV, PRV, or PPV. The dual ELISA could detect ASFV-infected positive serum samples up to dilutions of 5120 times, possessing high sensitivity. Therefore, the application of this dual ELISA approach can play an important role in ASFV epidemiology study and fill the gaps in differential diagnosis.Changjie LvYa ZhaoLili JiangLi ZhaoChao WuXianfeng HuiXiaotong HuZiqi ShaoXiaohan XiaXiaomei SunQiang ZhangMeilin JinMDPI AGarticleAfrican swine fever virus (ASFV)ELISAdifferentiationnon-hemadsorptionlower-virulence natural mutantsScienceQENLife, Vol 11, Iss 1214, p 1214 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	African swine fever virus (ASFV) ELISA differentiation non-hemadsorption lower-virulence natural mutants Science Q
spellingShingle	African swine fever virus (ASFV) ELISA differentiation non-hemadsorption lower-virulence natural mutants Science Q Changjie Lv Ya Zhao Lili Jiang Li Zhao Chao Wu Xianfeng Hui Xiaotong Hu Ziqi Shao Xiaohan Xia Xiaomei Sun Qiang Zhang Meilin Jin Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV
description	African swine fever virus (ASFV) is an important viral pathogen infecting pigs worldwide throughout the pig industry. CD2v (an outer-membrane glycosylated protein of ASFV)-unexpressed lower-virulence mutants have appeared in China and other countries in recent years. Using OIE-recommended quantitative PCR and ELISA methods, people can accurately judge whether pigs are infected with wild-type ASFV. However, the strategy has failed to distinguish ΔCD2v lower-virulence mutants and wild-type ASFV infection. Here, we expressed and purified the CD2v and p30 proteins via CHO cells and successfully established a dual enzyme-linked immunosorbent assay (ELISA), which can be used to differentiate pigs infected with wild-type ASFV or with CD2v-unexpressed lower-virulence mutants. The dual ELISA showed excellent specificity without cross-reactions with antibodies of PRRSV, CSFV, JEV, PRV, or PPV. The dual ELISA could detect ASFV-infected positive serum samples up to dilutions of 5120 times, possessing high sensitivity. Therefore, the application of this dual ELISA approach can play an important role in ASFV epidemiology study and fill the gaps in differential diagnosis.
format	article
author	Changjie Lv Ya Zhao Lili Jiang Li Zhao Chao Wu Xianfeng Hui Xiaotong Hu Ziqi Shao Xiaohan Xia Xiaomei Sun Qiang Zhang Meilin Jin
author_facet	Changjie Lv Ya Zhao Lili Jiang Li Zhao Chao Wu Xianfeng Hui Xiaotong Hu Ziqi Shao Xiaohan Xia Xiaomei Sun Qiang Zhang Meilin Jin
author_sort	Changjie Lv
title	Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV
title_short	Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV
title_full	Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV
title_fullStr	Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV
title_full_unstemmed	Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV
title_sort	development of a dual elisa for the detection of cd2v-unexpressed lower-virulence mutational asfv
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fb8b0ee8709d444b93c0be364b88f0cf
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Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV

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