Male-specific association between dopamine receptor D4 gene methylation and schizophrenia.

<h4>Objective</h4>The goal of our study was to investigate whether DRD4 gene DNA methylation played an important role in the susceptibility of Han Chinese SCZ.<h4>Methods</h4>Using the bisulphite pyrosequencing technology, DNA methylation levels of 6 CpG dinucleotides in DRD4...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Jia Cheng, Yunliang Wang, Kena Zhou, Lingyan Wang, Jinfeng Li, Qidong Zhuang, Xuting Xu, Leiting Xu, Kai Zhang, Dongjun Dai, Rongjiong Zheng, Guangxue Li, Aiping Zhang, Shugui Gao, Shiwei Duan
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
R
Q
Acceso en línea:https://doaj.org/article/fb91755c40de456d860659423b1ce1f5
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:fb91755c40de456d860659423b1ce1f5
record_format dspace
spelling oai:doaj.org-article:fb91755c40de456d860659423b1ce1f52021-11-18T08:31:59ZMale-specific association between dopamine receptor D4 gene methylation and schizophrenia.1932-620310.1371/journal.pone.0089128https://doaj.org/article/fb91755c40de456d860659423b1ce1f52014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24586542/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>The goal of our study was to investigate whether DRD4 gene DNA methylation played an important role in the susceptibility of Han Chinese SCZ.<h4>Methods</h4>Using the bisulphite pyrosequencing technology, DNA methylation levels of 6 CpG dinucleotides in DRD4 CpG island were measured among 30 paranoid SCZ patients, 30 undifferentiated SCZ patients, and 30 age- and gender-matched healthy controls.<h4>Results</h4>Strong correlation was observed among the six CpG sites (r>0.5, P<0.01), thus average methylation levels were applied thereafter. Our results indicated that there was a significant association between DRD4 methylation and the risk of SCZ (P = 0.003), although there was no significant difference in DRD4 methylation between the two SCZ subtypes (P = 0.670). A breakdown analysis by gender showed that the significant association of DRD4 methylation and SCZ was driven by males (P<0.001) but not by females (P = 0.835). DRD4 methylation was significantly associated with p300 in male SCZ patients (r = -0.543, P = 0.005) but not in female SCZ patients (r = 0.110, P = 0.599). Moreover, receiver operating characteristic (ROC) curves showed DRD4 methylation was able to predict the status of SCZ in males [area under curve (AUC) = 0.832, P = 0.002] but not in females (AUC = 0.483, P = 0.876). Finally, a further expression experiment showed that DRD4 methylation in the gene body was positively associated with gene expression, although the exact mechanism of gene regulation remained unknown for this interesting DRD4 methylation.<h4>Conclusion</h4>The gender disparity in the DRD4 DNA methylation provides novel insights into the pathogenesis of SCZ.Jia ChengYunliang WangKena ZhouLingyan WangJinfeng LiQidong ZhuangXuting XuLeiting XuKai ZhangDongjun DaiRongjiong ZhengGuangxue LiAiping ZhangShugui GaoShiwei DuanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e89128 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jia Cheng
Yunliang Wang
Kena Zhou
Lingyan Wang
Jinfeng Li
Qidong Zhuang
Xuting Xu
Leiting Xu
Kai Zhang
Dongjun Dai
Rongjiong Zheng
Guangxue Li
Aiping Zhang
Shugui Gao
Shiwei Duan
Male-specific association between dopamine receptor D4 gene methylation and schizophrenia.
description <h4>Objective</h4>The goal of our study was to investigate whether DRD4 gene DNA methylation played an important role in the susceptibility of Han Chinese SCZ.<h4>Methods</h4>Using the bisulphite pyrosequencing technology, DNA methylation levels of 6 CpG dinucleotides in DRD4 CpG island were measured among 30 paranoid SCZ patients, 30 undifferentiated SCZ patients, and 30 age- and gender-matched healthy controls.<h4>Results</h4>Strong correlation was observed among the six CpG sites (r>0.5, P<0.01), thus average methylation levels were applied thereafter. Our results indicated that there was a significant association between DRD4 methylation and the risk of SCZ (P = 0.003), although there was no significant difference in DRD4 methylation between the two SCZ subtypes (P = 0.670). A breakdown analysis by gender showed that the significant association of DRD4 methylation and SCZ was driven by males (P<0.001) but not by females (P = 0.835). DRD4 methylation was significantly associated with p300 in male SCZ patients (r = -0.543, P = 0.005) but not in female SCZ patients (r = 0.110, P = 0.599). Moreover, receiver operating characteristic (ROC) curves showed DRD4 methylation was able to predict the status of SCZ in males [area under curve (AUC) = 0.832, P = 0.002] but not in females (AUC = 0.483, P = 0.876). Finally, a further expression experiment showed that DRD4 methylation in the gene body was positively associated with gene expression, although the exact mechanism of gene regulation remained unknown for this interesting DRD4 methylation.<h4>Conclusion</h4>The gender disparity in the DRD4 DNA methylation provides novel insights into the pathogenesis of SCZ.
format article
author Jia Cheng
Yunliang Wang
Kena Zhou
Lingyan Wang
Jinfeng Li
Qidong Zhuang
Xuting Xu
Leiting Xu
Kai Zhang
Dongjun Dai
Rongjiong Zheng
Guangxue Li
Aiping Zhang
Shugui Gao
Shiwei Duan
author_facet Jia Cheng
Yunliang Wang
Kena Zhou
Lingyan Wang
Jinfeng Li
Qidong Zhuang
Xuting Xu
Leiting Xu
Kai Zhang
Dongjun Dai
Rongjiong Zheng
Guangxue Li
Aiping Zhang
Shugui Gao
Shiwei Duan
author_sort Jia Cheng
title Male-specific association between dopamine receptor D4 gene methylation and schizophrenia.
title_short Male-specific association between dopamine receptor D4 gene methylation and schizophrenia.
title_full Male-specific association between dopamine receptor D4 gene methylation and schizophrenia.
title_fullStr Male-specific association between dopamine receptor D4 gene methylation and schizophrenia.
title_full_unstemmed Male-specific association between dopamine receptor D4 gene methylation and schizophrenia.
title_sort male-specific association between dopamine receptor d4 gene methylation and schizophrenia.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/fb91755c40de456d860659423b1ce1f5
work_keys_str_mv AT jiacheng malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT yunliangwang malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT kenazhou malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT lingyanwang malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT jinfengli malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT qidongzhuang malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT xutingxu malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT leitingxu malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT kaizhang malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT dongjundai malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT rongjiongzheng malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT guangxueli malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT aipingzhang malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT shuguigao malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
AT shiweiduan malespecificassociationbetweendopaminereceptord4genemethylationandschizophrenia
_version_ 1718421720415600640