MEF2C shapes the microtranscriptome during differentiation of skeletal muscles

Abstract Myocyte enhancer factor 2C (MEF2C) is a transcription factor that regulates heart and skeletal muscle differentiation and growth. Several protein-encoding genes were identified as targets of this factor; however, little is known about its contribution to the microtranscriptome composition a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Agnieszka Piasecka, Michał Sekrecki, Michał Wojciech Szcześniak, Krzysztof Sobczak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb929136498a4e399644d825635f831a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fb929136498a4e399644d825635f831a
record_format	dspace
spelling	oai:doaj.org-article:fb929136498a4e399644d825635f831a2021-12-02T12:09:26ZMEF2C shapes the microtranscriptome during differentiation of skeletal muscles10.1038/s41598-021-82706-22045-2322https://doaj.org/article/fb929136498a4e399644d825635f831a2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82706-2https://doaj.org/toc/2045-2322Abstract Myocyte enhancer factor 2C (MEF2C) is a transcription factor that regulates heart and skeletal muscle differentiation and growth. Several protein-encoding genes were identified as targets of this factor; however, little is known about its contribution to the microtranscriptome composition and dynamics in myogenic programs. In this report, we aimed to address this question. Deep sequencing of small RNAs of human muscle cells revealed a set of microRNAs (miRNAs), including several muscle-specific miRNAs, that are sensitive to MEF2C depletion. As expected, in cells with knockdown of MEF2C, we found mostly downregulated miRNAs; nevertheless, as much as one-third of altered miRNAs were upregulated. The majority of these changes are driven by transcription efficiency. Moreover, we found that MEF2C affects nontemplated 3′-end nucleotide addition of miRNAs, mainly oligouridylation. The rate of these modifications is associated with the level of TUT4 which mediates RNA 3′-uridylation. Finally, we found that a quarter of miRNAs which significantly changed upon differentiation of human skeletal myoblasts is inversely altered in MEF2C deficient cells. We concluded that MEF2C is an essential factor regulating both the quantity and quality of the microtranscriptome, leaving an imprint on the stability and perhaps specificity of many miRNAs during the differentiation of muscle cells.Agnieszka PiaseckaMichał SekreckiMichał Wojciech SzcześniakKrzysztof SobczakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Agnieszka Piasecka Michał Sekrecki Michał Wojciech Szcześniak Krzysztof Sobczak MEF2C shapes the microtranscriptome during differentiation of skeletal muscles
description	Abstract Myocyte enhancer factor 2C (MEF2C) is a transcription factor that regulates heart and skeletal muscle differentiation and growth. Several protein-encoding genes were identified as targets of this factor; however, little is known about its contribution to the microtranscriptome composition and dynamics in myogenic programs. In this report, we aimed to address this question. Deep sequencing of small RNAs of human muscle cells revealed a set of microRNAs (miRNAs), including several muscle-specific miRNAs, that are sensitive to MEF2C depletion. As expected, in cells with knockdown of MEF2C, we found mostly downregulated miRNAs; nevertheless, as much as one-third of altered miRNAs were upregulated. The majority of these changes are driven by transcription efficiency. Moreover, we found that MEF2C affects nontemplated 3′-end nucleotide addition of miRNAs, mainly oligouridylation. The rate of these modifications is associated with the level of TUT4 which mediates RNA 3′-uridylation. Finally, we found that a quarter of miRNAs which significantly changed upon differentiation of human skeletal myoblasts is inversely altered in MEF2C deficient cells. We concluded that MEF2C is an essential factor regulating both the quantity and quality of the microtranscriptome, leaving an imprint on the stability and perhaps specificity of many miRNAs during the differentiation of muscle cells.
format	article
author	Agnieszka Piasecka Michał Sekrecki Michał Wojciech Szcześniak Krzysztof Sobczak
author_facet	Agnieszka Piasecka Michał Sekrecki Michał Wojciech Szcześniak Krzysztof Sobczak
author_sort	Agnieszka Piasecka
title	MEF2C shapes the microtranscriptome during differentiation of skeletal muscles
title_short	MEF2C shapes the microtranscriptome during differentiation of skeletal muscles
title_full	MEF2C shapes the microtranscriptome during differentiation of skeletal muscles
title_fullStr	MEF2C shapes the microtranscriptome during differentiation of skeletal muscles
title_full_unstemmed	MEF2C shapes the microtranscriptome during differentiation of skeletal muscles
title_sort	mef2c shapes the microtranscriptome during differentiation of skeletal muscles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fb929136498a4e399644d825635f831a
work_keys_str_mv	AT agnieszkapiasecka mef2cshapesthemicrotranscriptomeduringdifferentiationofskeletalmuscles AT michałsekrecki mef2cshapesthemicrotranscriptomeduringdifferentiationofskeletalmuscles AT michałwojciechszczesniak mef2cshapesthemicrotranscriptomeduringdifferentiationofskeletalmuscles AT krzysztofsobczak mef2cshapesthemicrotranscriptomeduringdifferentiationofskeletalmuscles
_version_	1718394668795822080

MEF2C shapes the microtranscriptome during differentiation of skeletal muscles

Ejemplares similares