Mitochondrial fission, integrity and completion of mitophagy require separable functions of Vps13D in Drosophila neurons.

A healthy population of mitochondria, maintained by proper fission, fusion, and degradation, is critical for the long-term survival and function of neurons. Here, our discovery of mitophagy intermediates in fission-impaired Drosophila neurons brings new perspective into the relationship between mito...

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Autores principales:	Ryan Insolera, Péter Lőrincz, Alec J Wishnie, Gábor Juhász, Catherine A Collins
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/fb952655066e470d9071d84e4bb004ea
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spelling	oai:doaj.org-article:fb952655066e470d9071d84e4bb004ea2021-12-02T20:02:52ZMitochondrial fission, integrity and completion of mitophagy require separable functions of Vps13D in Drosophila neurons.1553-73901553-740410.1371/journal.pgen.1009731https://doaj.org/article/fb952655066e470d9071d84e4bb004ea2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009731https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404A healthy population of mitochondria, maintained by proper fission, fusion, and degradation, is critical for the long-term survival and function of neurons. Here, our discovery of mitophagy intermediates in fission-impaired Drosophila neurons brings new perspective into the relationship between mitochondrial fission and mitophagy. Neurons lacking either the ataxia disease gene Vps13D or the dynamin related protein Drp1 contain enlarged mitochondria that are engaged with autophagy machinery and also lack matrix components. Reporter assays combined with genetic studies imply that mitophagy both initiates and is completed in Drp1 impaired neurons, but fails to complete in Vps13D impaired neurons, which accumulate compromised mitochondria within stalled mito-phagophores. Our findings imply that in fission-defective neurons, mitophagy becomes induced, and that the lipid channel containing protein Vps13D has separable functions in mitochondrial fission and phagophore elongation.Ryan InsoleraPéter LőrinczAlec J WishnieGábor JuhászCatherine A CollinsPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009731 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Ryan Insolera Péter Lőrincz Alec J Wishnie Gábor Juhász Catherine A Collins Mitochondrial fission, integrity and completion of mitophagy require separable functions of Vps13D in Drosophila neurons.
description	A healthy population of mitochondria, maintained by proper fission, fusion, and degradation, is critical for the long-term survival and function of neurons. Here, our discovery of mitophagy intermediates in fission-impaired Drosophila neurons brings new perspective into the relationship between mitochondrial fission and mitophagy. Neurons lacking either the ataxia disease gene Vps13D or the dynamin related protein Drp1 contain enlarged mitochondria that are engaged with autophagy machinery and also lack matrix components. Reporter assays combined with genetic studies imply that mitophagy both initiates and is completed in Drp1 impaired neurons, but fails to complete in Vps13D impaired neurons, which accumulate compromised mitochondria within stalled mito-phagophores. Our findings imply that in fission-defective neurons, mitophagy becomes induced, and that the lipid channel containing protein Vps13D has separable functions in mitochondrial fission and phagophore elongation.
format	article
author	Ryan Insolera Péter Lőrincz Alec J Wishnie Gábor Juhász Catherine A Collins
author_facet	Ryan Insolera Péter Lőrincz Alec J Wishnie Gábor Juhász Catherine A Collins
author_sort	Ryan Insolera
title	Mitochondrial fission, integrity and completion of mitophagy require separable functions of Vps13D in Drosophila neurons.
title_short	Mitochondrial fission, integrity and completion of mitophagy require separable functions of Vps13D in Drosophila neurons.
title_full	Mitochondrial fission, integrity and completion of mitophagy require separable functions of Vps13D in Drosophila neurons.
title_fullStr	Mitochondrial fission, integrity and completion of mitophagy require separable functions of Vps13D in Drosophila neurons.
title_full_unstemmed	Mitochondrial fission, integrity and completion of mitophagy require separable functions of Vps13D in Drosophila neurons.
title_sort	mitochondrial fission, integrity and completion of mitophagy require separable functions of vps13d in drosophila neurons.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/fb952655066e470d9071d84e4bb004ea
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Mitochondrial fission, integrity and completion of mitophagy require separable functions of Vps13D in Drosophila neurons.

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