Hybrid bilayer membranes on metallurgical polished aluminum

Abstract In this work we describe the functionalization of metallurgically polished aluminum surfaces yielding biomimetic electrodes suitable for probing protein/phospholipid interactions. The functionalization involves two simple steps: silanization of the aluminum and subsequent fusion of multilam...

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Autores principales:	Tomas Sabirovas, Aušra Valiūnienė, Gintaras Valincius
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb9ba7c13dc348c7a4bbcd1d8d1d3b43
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spelling	oai:doaj.org-article:fb9ba7c13dc348c7a4bbcd1d8d1d3b432021-12-02T14:49:33ZHybrid bilayer membranes on metallurgical polished aluminum10.1038/s41598-021-89150-22045-2322https://doaj.org/article/fb9ba7c13dc348c7a4bbcd1d8d1d3b432021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89150-2https://doaj.org/toc/2045-2322Abstract In this work we describe the functionalization of metallurgically polished aluminum surfaces yielding biomimetic electrodes suitable for probing protein/phospholipid interactions. The functionalization involves two simple steps: silanization of the aluminum and subsequent fusion of multilamellar vesicles which leads to the formation of a hybrid bilayer lipid membrane (hBLM). The vesicle fusion was followed in real-time by fast Fourier transform electrochemical impedance spectroscopy (FFT EIS). The impedance-derived complex capacitance of the hBLMs was approximately 0.61 µF cm−2, a value typical for intact phospholipid bilayers. We found that the hBLMs can be readily disrupted if exposed to > 400 nM solutions of the pore-forming peptide melittin. However, the presence of cholesterol at 40% (mol) in hBLMs exhibited an inhibitory effect on the membrane-damaging capacity of the peptide. The melittin-membrane interaction was concentration dependent decreasing with concentration. The hBLMs on Al surface can be regenerated multiple times, retaining their dielectric and functional properties essentially intact.Tomas SabirovasAušra ValiūnienėGintaras ValinciusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomas Sabirovas Aušra Valiūnienė Gintaras Valincius Hybrid bilayer membranes on metallurgical polished aluminum
description	Abstract In this work we describe the functionalization of metallurgically polished aluminum surfaces yielding biomimetic electrodes suitable for probing protein/phospholipid interactions. The functionalization involves two simple steps: silanization of the aluminum and subsequent fusion of multilamellar vesicles which leads to the formation of a hybrid bilayer lipid membrane (hBLM). The vesicle fusion was followed in real-time by fast Fourier transform electrochemical impedance spectroscopy (FFT EIS). The impedance-derived complex capacitance of the hBLMs was approximately 0.61 µF cm−2, a value typical for intact phospholipid bilayers. We found that the hBLMs can be readily disrupted if exposed to > 400 nM solutions of the pore-forming peptide melittin. However, the presence of cholesterol at 40% (mol) in hBLMs exhibited an inhibitory effect on the membrane-damaging capacity of the peptide. The melittin-membrane interaction was concentration dependent decreasing with concentration. The hBLMs on Al surface can be regenerated multiple times, retaining their dielectric and functional properties essentially intact.
format	article
author	Tomas Sabirovas Aušra Valiūnienė Gintaras Valincius
author_facet	Tomas Sabirovas Aušra Valiūnienė Gintaras Valincius
author_sort	Tomas Sabirovas
title	Hybrid bilayer membranes on metallurgical polished aluminum
title_short	Hybrid bilayer membranes on metallurgical polished aluminum
title_full	Hybrid bilayer membranes on metallurgical polished aluminum
title_fullStr	Hybrid bilayer membranes on metallurgical polished aluminum
title_full_unstemmed	Hybrid bilayer membranes on metallurgical polished aluminum
title_sort	hybrid bilayer membranes on metallurgical polished aluminum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fb9ba7c13dc348c7a4bbcd1d8d1d3b43
work_keys_str_mv	AT tomassabirovas hybridbilayermembranesonmetallurgicalpolishedaluminum AT ausravaliuniene hybridbilayermembranesonmetallurgicalpolishedaluminum AT gintarasvalincius hybridbilayermembranesonmetallurgicalpolishedaluminum
_version_	1718389487152660480

Hybrid bilayer membranes on metallurgical polished aluminum

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