Pathogenic Neisseria hitchhike on the uropod of human neutrophils.

Polymorphonuclear neutrophils (PMNs) are important components of the human innate immune system and are rapidly recruited at the site of bacterial infection. Despite the effective phagocytic activity of PMNs, Neisseria gonorrhoeae infections are characterized by high survival within PMNs. We reveal...

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Autores principales:	Niklas Söderholm, Katarina Vielfort, Kjell Hultenby, Helena Aro
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fb9cf7901e3a4cc79ebecd438bdf5ebd
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spelling	oai:doaj.org-article:fb9cf7901e3a4cc79ebecd438bdf5ebd2021-11-04T06:08:24ZPathogenic Neisseria hitchhike on the uropod of human neutrophils.1932-620310.1371/journal.pone.0024353https://doaj.org/article/fb9cf7901e3a4cc79ebecd438bdf5ebd2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21949708/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Polymorphonuclear neutrophils (PMNs) are important components of the human innate immune system and are rapidly recruited at the site of bacterial infection. Despite the effective phagocytic activity of PMNs, Neisseria gonorrhoeae infections are characterized by high survival within PMNs. We reveal a novel type IV pilus-mediated adherence of pathogenic Neisseria to the uropod (the rear) of polarized PMNs. The direct pilus-uropod interaction was visualized by scanning electron microscopy and total internal reflection fluorescence (TIRF) microscopy. We showed that N. meningitidis adhesion to the PMN uropod depended on both pilus-associated proteins PilC1 and PilC2, while N. gonorrhoeae adhesion did not. Bacterial adhesion elicited accumulation of the complement regulator CD46, but not I-domain-containing integrins, beneath the adherent bacterial microcolony. Electrographs and live-cell imaging of PMNs suggested that bacterial adherence to the uropod is followed by internalization into PMNs via the uropod. We also present data showing that pathogenic Neisseria can hitchhike on PMNs to hide from their phagocytic activity as well as to facilitate the spread of the pathogen through the epithelial cell layer.Niklas SöderholmKatarina VielfortKjell HultenbyHelena AroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24353 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Niklas Söderholm Katarina Vielfort Kjell Hultenby Helena Aro Pathogenic Neisseria hitchhike on the uropod of human neutrophils.
description	Polymorphonuclear neutrophils (PMNs) are important components of the human innate immune system and are rapidly recruited at the site of bacterial infection. Despite the effective phagocytic activity of PMNs, Neisseria gonorrhoeae infections are characterized by high survival within PMNs. We reveal a novel type IV pilus-mediated adherence of pathogenic Neisseria to the uropod (the rear) of polarized PMNs. The direct pilus-uropod interaction was visualized by scanning electron microscopy and total internal reflection fluorescence (TIRF) microscopy. We showed that N. meningitidis adhesion to the PMN uropod depended on both pilus-associated proteins PilC1 and PilC2, while N. gonorrhoeae adhesion did not. Bacterial adhesion elicited accumulation of the complement regulator CD46, but not I-domain-containing integrins, beneath the adherent bacterial microcolony. Electrographs and live-cell imaging of PMNs suggested that bacterial adherence to the uropod is followed by internalization into PMNs via the uropod. We also present data showing that pathogenic Neisseria can hitchhike on PMNs to hide from their phagocytic activity as well as to facilitate the spread of the pathogen through the epithelial cell layer.
format	article
author	Niklas Söderholm Katarina Vielfort Kjell Hultenby Helena Aro
author_facet	Niklas Söderholm Katarina Vielfort Kjell Hultenby Helena Aro
author_sort	Niklas Söderholm
title	Pathogenic Neisseria hitchhike on the uropod of human neutrophils.
title_short	Pathogenic Neisseria hitchhike on the uropod of human neutrophils.
title_full	Pathogenic Neisseria hitchhike on the uropod of human neutrophils.
title_fullStr	Pathogenic Neisseria hitchhike on the uropod of human neutrophils.
title_full_unstemmed	Pathogenic Neisseria hitchhike on the uropod of human neutrophils.
title_sort	pathogenic neisseria hitchhike on the uropod of human neutrophils.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/fb9cf7901e3a4cc79ebecd438bdf5ebd
work_keys_str_mv	AT niklassoderholm pathogenicneisseriahitchhikeontheuropodofhumanneutrophils AT katarinavielfort pathogenicneisseriahitchhikeontheuropodofhumanneutrophils AT kjellhultenby pathogenicneisseriahitchhikeontheuropodofhumanneutrophils AT helenaaro pathogenicneisseriahitchhikeontheuropodofhumanneutrophils
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Pathogenic Neisseria hitchhike on the uropod of human neutrophils.

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