Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma

Abstract Recurrence in oral squamous cell carcinoma (OSCC) significantly reduces overall survival. Improved understanding of the host’s immune status in head and neck cancer may facilitate identification of patients at higher risk of recurrence and improve patients’ selection for ongoing clinical tr...

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Autores principales:	Åsa Kågedal, Eric Hjalmarsson, Pedro Farrajota Neves da Silva, Krzysztof Piersiala, Susanna Kumlien Georén, Gregori Margolin, Eva Munck-Wikland, Ola Winqvist, Valtteri Häyry, Lars Olaf Cardell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fba114c3634743a6a7d2412014d261d1
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spelling	oai:doaj.org-article:fba114c3634743a6a7d2412014d261d12021-12-02T13:34:00ZActivation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma10.1038/s41598-020-79273-32045-2322https://doaj.org/article/fba114c3634743a6a7d2412014d261d12020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79273-3https://doaj.org/toc/2045-2322Abstract Recurrence in oral squamous cell carcinoma (OSCC) significantly reduces overall survival. Improved understanding of the host’s immune status in head and neck cancer may facilitate identification of patients at higher risk of recurrence and improve patients’ selection for ongoing clinical trials assessing the effectiveness of immune checkpoint inhibitors (CPI). We aimed to investigate Sentinel Node-derived T-cells and their impact on survival. We enrolled prospectively 28 OSCC patients treated at Karolinska University Hospital, Stockholm, Sweden with primary tumour excision and elective neck dissection. On top of the standard treatment, the enrolled patients underwent sentinel node procedure. T cells derived from Sentinel nodes, non-sentinel nodes, primary tumour and PBMC were analyzed in flow cytometry. Patients who developed recurrence proved to have significantly lower level of CD4+ CD69+ in their sentinel node (31.38 ± 6.019% vs. 43.44 ± 15.33%, p = 0.0103) and significantly higher level of CD8+ CD HLA-DR+ (38.95 ± 9.479% vs. 24.58 ± 11.36%, p = 0.0116) compared to disease-free individuals. Survival analysis of studied population revealed that patients with low proportion of CD4+ CD69+ had significantly decreased disease-free survival (DFS) of 19.7 months (95% CI 12.6–26.9) compared with 42.6 months (95% CI 40.1–45.1) in those with high CD4+ CD69+ proportion in their Sentinel Nodes (log-rank test, p = 0.033). Our findings demonstrate that characterization of T-cell activation in Sentinel Node serves as a complementary prognostic marker. Flow cytometry of Sentinel Node may be useful in both patients’ surveillance and selection for ongoing CPI clinical trials in head and neck cancer.Åsa KågedalEric HjalmarssonPedro Farrajota Neves da SilvaKrzysztof PiersialaSusanna Kumlien GeorénGregori MargolinEva Munck-WiklandOla WinqvistValtteri HäyryLars Olaf CardellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Åsa Kågedal Eric Hjalmarsson Pedro Farrajota Neves da Silva Krzysztof Piersiala Susanna Kumlien Georén Gregori Margolin Eva Munck-Wikland Ola Winqvist Valtteri Häyry Lars Olaf Cardell Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma
description	Abstract Recurrence in oral squamous cell carcinoma (OSCC) significantly reduces overall survival. Improved understanding of the host’s immune status in head and neck cancer may facilitate identification of patients at higher risk of recurrence and improve patients’ selection for ongoing clinical trials assessing the effectiveness of immune checkpoint inhibitors (CPI). We aimed to investigate Sentinel Node-derived T-cells and their impact on survival. We enrolled prospectively 28 OSCC patients treated at Karolinska University Hospital, Stockholm, Sweden with primary tumour excision and elective neck dissection. On top of the standard treatment, the enrolled patients underwent sentinel node procedure. T cells derived from Sentinel nodes, non-sentinel nodes, primary tumour and PBMC were analyzed in flow cytometry. Patients who developed recurrence proved to have significantly lower level of CD4+ CD69+ in their sentinel node (31.38 ± 6.019% vs. 43.44 ± 15.33%, p = 0.0103) and significantly higher level of CD8+ CD HLA-DR+ (38.95 ± 9.479% vs. 24.58 ± 11.36%, p = 0.0116) compared to disease-free individuals. Survival analysis of studied population revealed that patients with low proportion of CD4+ CD69+ had significantly decreased disease-free survival (DFS) of 19.7 months (95% CI 12.6–26.9) compared with 42.6 months (95% CI 40.1–45.1) in those with high CD4+ CD69+ proportion in their Sentinel Nodes (log-rank test, p = 0.033). Our findings demonstrate that characterization of T-cell activation in Sentinel Node serves as a complementary prognostic marker. Flow cytometry of Sentinel Node may be useful in both patients’ surveillance and selection for ongoing CPI clinical trials in head and neck cancer.
format	article
author	Åsa Kågedal Eric Hjalmarsson Pedro Farrajota Neves da Silva Krzysztof Piersiala Susanna Kumlien Georén Gregori Margolin Eva Munck-Wikland Ola Winqvist Valtteri Häyry Lars Olaf Cardell
author_facet	Åsa Kågedal Eric Hjalmarsson Pedro Farrajota Neves da Silva Krzysztof Piersiala Susanna Kumlien Georén Gregori Margolin Eva Munck-Wikland Ola Winqvist Valtteri Häyry Lars Olaf Cardell
author_sort	Åsa Kågedal
title	Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma
title_short	Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma
title_full	Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma
title_fullStr	Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma
title_full_unstemmed	Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma
title_sort	activation of t helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/fba114c3634743a6a7d2412014d261d1
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Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma

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