RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis

Abstract RNA-Seq is a powerful tool in transcriptomic profiling of cells and tissues. We recently identified many more taste buds than previously appreciated in chickens using molecular markers to stain oral epithelial sheets of the palate, base of oral cavity, and posterior tongue. In this study, R...

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Autores principales:	Xiaogang Cui, Brett Marshall, Ning Shi, Shi-You Chen, Romdhane Rekaya, Hong-Xiang Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fba90db998bd43a388f001c1bec00c67
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spelling	oai:doaj.org-article:fba90db998bd43a388f001c1bec00c672021-12-02T16:07:43ZRNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis10.1038/s41598-017-09299-72045-2322https://doaj.org/article/fba90db998bd43a388f001c1bec00c672017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09299-7https://doaj.org/toc/2045-2322Abstract RNA-Seq is a powerful tool in transcriptomic profiling of cells and tissues. We recently identified many more taste buds than previously appreciated in chickens using molecular markers to stain oral epithelial sheets of the palate, base of oral cavity, and posterior tongue. In this study, RNA-Seq was performed to understand the transcriptomic architecture of chicken gustatory tissues. Interestingly, taste sensation related genes and many more differentially expressed genes (DEGs) were found between the epithelium and mesenchyme in the base of oral cavity as compared to the palate and posterior tongue. Further RNA-Seq using specifically defined tissues of the base of oral cavity demonstrated that DEGs between gustatory (GE) and non-gustatory epithelium (NGE), and between GE and the underlying mesenchyme (GM) were enriched in multiple GO terms and KEGG pathways, including many biological processes. Well-known genes for taste sensation were highly expressed in the GE. Moreover, genes of signaling components important in organogenesis (Wnt, TGFβ/ BMP, FGF, Notch, SHH, Erbb) were differentially expressed between GE and GM. Combined with other features of chicken taste buds, e.g., uniquely patterned array and short turnover cycle, our data suggest that chicken gustatory tissue provides an ideal system for multidisciplinary studies, including organogenesis and regenerative medicine.Xiaogang CuiBrett MarshallNing ShiShi-You ChenRomdhane RekayaHong-Xiang LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaogang Cui Brett Marshall Ning Shi Shi-You Chen Romdhane Rekaya Hong-Xiang Liu RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis
description	Abstract RNA-Seq is a powerful tool in transcriptomic profiling of cells and tissues. We recently identified many more taste buds than previously appreciated in chickens using molecular markers to stain oral epithelial sheets of the palate, base of oral cavity, and posterior tongue. In this study, RNA-Seq was performed to understand the transcriptomic architecture of chicken gustatory tissues. Interestingly, taste sensation related genes and many more differentially expressed genes (DEGs) were found between the epithelium and mesenchyme in the base of oral cavity as compared to the palate and posterior tongue. Further RNA-Seq using specifically defined tissues of the base of oral cavity demonstrated that DEGs between gustatory (GE) and non-gustatory epithelium (NGE), and between GE and the underlying mesenchyme (GM) were enriched in multiple GO terms and KEGG pathways, including many biological processes. Well-known genes for taste sensation were highly expressed in the GE. Moreover, genes of signaling components important in organogenesis (Wnt, TGFβ/ BMP, FGF, Notch, SHH, Erbb) were differentially expressed between GE and GM. Combined with other features of chicken taste buds, e.g., uniquely patterned array and short turnover cycle, our data suggest that chicken gustatory tissue provides an ideal system for multidisciplinary studies, including organogenesis and regenerative medicine.
format	article
author	Xiaogang Cui Brett Marshall Ning Shi Shi-You Chen Romdhane Rekaya Hong-Xiang Liu
author_facet	Xiaogang Cui Brett Marshall Ning Shi Shi-You Chen Romdhane Rekaya Hong-Xiang Liu
author_sort	Xiaogang Cui
title	RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis
title_short	RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis
title_full	RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis
title_fullStr	RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis
title_full_unstemmed	RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis
title_sort	rna-seq analysis on chicken taste sensory organs: an ideal system to study organogenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fba90db998bd43a388f001c1bec00c67
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RNA-Seq analysis on chicken taste sensory organs: An ideal system to study organogenesis

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