Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.

Following trauma of the adult brain or spinal cord the injured axons of central neurons fail to regenerate or if intact display only limited anatomical plasticity through sprouting. Adult cortical neurons forming the corticospinal tract (CST) normally have low levels of the neuronal calcium sensor-1...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ping K Yip, Liang-Fong Wong, Thomas A Sears, Rafael J Yáñez-Muñoz, Stephen B McMahon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fbad2dc100a547e1b8b369e16fe7f3b2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fbad2dc100a547e1b8b369e16fe7f3b2
record_format	dspace
spelling	oai:doaj.org-article:fbad2dc100a547e1b8b369e16fe7f3b22021-12-02T19:54:48ZCortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.1544-91731545-788510.1371/journal.pbio.1000399https://doaj.org/article/fbad2dc100a547e1b8b369e16fe7f3b22010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20585375/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Following trauma of the adult brain or spinal cord the injured axons of central neurons fail to regenerate or if intact display only limited anatomical plasticity through sprouting. Adult cortical neurons forming the corticospinal tract (CST) normally have low levels of the neuronal calcium sensor-1 (NCS1) protein. In primary cultured adult cortical neurons, the lentivector-induced overexpression of NCS1 induces neurite sprouting associated with increased phospho-Akt levels. When the PI3K/Akt signalling pathway was pharmacologically inhibited the NCS1-induced neurite sprouting was abolished. The overexpression of NCS1 in uninjured corticospinal neurons exhibited axonal sprouting across the midline into the CST-denervated side of the spinal cord following unilateral pyramidotomy. Improved forelimb function was demonstrated behaviourally and electrophysiologically. In injured corticospinal neurons, overexpression of NCS1 induced axonal sprouting and regeneration and also neuroprotection. These findings demonstrate that increasing the levels of intracellular NCS1 in injured and uninjured central neurons enhances their intrinsic anatomical plasticity within the injured adult central nervous system.Ping K YipLiang-Fong WongThomas A SearsRafael J Yáñez-MuñozStephen B McMahonPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 8, Iss 6, p e1000399 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Ping K Yip Liang-Fong Wong Thomas A Sears Rafael J Yáñez-Muñoz Stephen B McMahon Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.
description	Following trauma of the adult brain or spinal cord the injured axons of central neurons fail to regenerate or if intact display only limited anatomical plasticity through sprouting. Adult cortical neurons forming the corticospinal tract (CST) normally have low levels of the neuronal calcium sensor-1 (NCS1) protein. In primary cultured adult cortical neurons, the lentivector-induced overexpression of NCS1 induces neurite sprouting associated with increased phospho-Akt levels. When the PI3K/Akt signalling pathway was pharmacologically inhibited the NCS1-induced neurite sprouting was abolished. The overexpression of NCS1 in uninjured corticospinal neurons exhibited axonal sprouting across the midline into the CST-denervated side of the spinal cord following unilateral pyramidotomy. Improved forelimb function was demonstrated behaviourally and electrophysiologically. In injured corticospinal neurons, overexpression of NCS1 induced axonal sprouting and regeneration and also neuroprotection. These findings demonstrate that increasing the levels of intracellular NCS1 in injured and uninjured central neurons enhances their intrinsic anatomical plasticity within the injured adult central nervous system.
format	article
author	Ping K Yip Liang-Fong Wong Thomas A Sears Rafael J Yáñez-Muñoz Stephen B McMahon
author_facet	Ping K Yip Liang-Fong Wong Thomas A Sears Rafael J Yáñez-Muñoz Stephen B McMahon
author_sort	Ping K Yip
title	Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.
title_short	Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.
title_full	Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.
title_fullStr	Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.
title_full_unstemmed	Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.
title_sort	cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/fbad2dc100a547e1b8b369e16fe7f3b2
work_keys_str_mv	AT pingkyip corticaloverexpressionofneuronalcalciumsensor1inducesfunctionalplasticityinspinalcordfollowingunilateralpyramidaltractinjuryinrat AT liangfongwong corticaloverexpressionofneuronalcalciumsensor1inducesfunctionalplasticityinspinalcordfollowingunilateralpyramidaltractinjuryinrat AT thomasasears corticaloverexpressionofneuronalcalciumsensor1inducesfunctionalplasticityinspinalcordfollowingunilateralpyramidaltractinjuryinrat AT rafaeljyanezmunoz corticaloverexpressionofneuronalcalciumsensor1inducesfunctionalplasticityinspinalcordfollowingunilateralpyramidaltractinjuryinrat AT stephenbmcmahon corticaloverexpressionofneuronalcalciumsensor1inducesfunctionalplasticityinspinalcordfollowingunilateralpyramidaltractinjuryinrat
_version_	1718375879182123008

Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.

Ejemplares similares