Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria

Abstract. Inggraini M, Nurfajriah S, Priyanto JA, Ilsan NA. 2021. Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria. Biodiversitas 22: 555-562. Antibiotic is the first option treatment for infectious diseases both in human and animal. However...

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Autores principales:	MAULIN INGGRAINI, SITI NURFAJRIAH, JEPRI AGUNG PRIYANTO, Noor Andryan Ilsan
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	16s rrna, antibiotic, antimicrobial resistance, clinical bacteria, carbapenem-resistant bacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fbb6357f78a34355a0332870b9ee1989
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spelling	oai:doaj.org-article:fbb6357f78a34355a0332870b9ee19892021-11-22T00:54:56ZAntimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria1412-033X2085-472210.13057/biodiv/d220206https://doaj.org/article/fbb6357f78a34355a0332870b9ee19892021-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7189https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Inggraini M, Nurfajriah S, Priyanto JA, Ilsan NA. 2021. Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria. Biodiversitas 22: 555-562. Antibiotic is the first option treatment for infectious diseases both in human and animal. However, the excessive usage and misuse of antibiotics have driven antibacterial resistances worldwide and the increasing case of antibiotic resistance leads to limited options for treatment. This study aimed to observe antimicrobial susceptibility and molecular identification of carbapenem-resistant human clinical bacteria. A total of nine isolates in this study were collected in 2020 from a teaching hospital in Indonesia. All isolates were originated from various human clinical specimens, including urine, blood, pus, and sputum. Identification using 16s rRNA-based showed that these isolates were closely related to Klebsiella pneumoniae (1/9), A. baumannii (5/9), Escherichia coli (2/9), and Lysinibacillus fusiformis (1/9). According to minimum inhibitory concentration using Vitek Automated Machine, four isolates of multi-drug resistant (MDR) bacteria were found. In contrast, five of them were categorized as extensively-drug resistant (XDR). Interestingly, all of the XDR isolates belonged to A. baumannii. These isolates were resistant to at least seven different antimicrobial classes. A comparison of partial 16s rRNA showed two E. coli had similar variance. While in A. baumannii isolates, we found one of five isolates had a different variance sequence, which suggests different clonality among this species. This study gives an insight into the prevalence of carbapenem-resistant bacteria with XDR criteria in Indonesia.MAULIN INGGRAINISITI NURFAJRIAHJEPRI AGUNG PRIYANTONoor Andryan IlsanMBI & UNS Soloarticle16s rrna, antibiotic, antimicrobial resistance, clinical bacteria, carbapenem-resistant bacteriaBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna, antibiotic, antimicrobial resistance, clinical bacteria, carbapenem-resistant bacteria Biology (General) QH301-705.5
spellingShingle	16s rrna, antibiotic, antimicrobial resistance, clinical bacteria, carbapenem-resistant bacteria Biology (General) QH301-705.5 MAULIN INGGRAINI SITI NURFAJRIAH JEPRI AGUNG PRIYANTO Noor Andryan Ilsan Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria
description	Abstract. Inggraini M, Nurfajriah S, Priyanto JA, Ilsan NA. 2021. Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria. Biodiversitas 22: 555-562. Antibiotic is the first option treatment for infectious diseases both in human and animal. However, the excessive usage and misuse of antibiotics have driven antibacterial resistances worldwide and the increasing case of antibiotic resistance leads to limited options for treatment. This study aimed to observe antimicrobial susceptibility and molecular identification of carbapenem-resistant human clinical bacteria. A total of nine isolates in this study were collected in 2020 from a teaching hospital in Indonesia. All isolates were originated from various human clinical specimens, including urine, blood, pus, and sputum. Identification using 16s rRNA-based showed that these isolates were closely related to Klebsiella pneumoniae (1/9), A. baumannii (5/9), Escherichia coli (2/9), and Lysinibacillus fusiformis (1/9). According to minimum inhibitory concentration using Vitek Automated Machine, four isolates of multi-drug resistant (MDR) bacteria were found. In contrast, five of them were categorized as extensively-drug resistant (XDR). Interestingly, all of the XDR isolates belonged to A. baumannii. These isolates were resistant to at least seven different antimicrobial classes. A comparison of partial 16s rRNA showed two E. coli had similar variance. While in A. baumannii isolates, we found one of five isolates had a different variance sequence, which suggests different clonality among this species. This study gives an insight into the prevalence of carbapenem-resistant bacteria with XDR criteria in Indonesia.
format	article
author	MAULIN INGGRAINI SITI NURFAJRIAH JEPRI AGUNG PRIYANTO Noor Andryan Ilsan
author_facet	MAULIN INGGRAINI SITI NURFAJRIAH JEPRI AGUNG PRIYANTO Noor Andryan Ilsan
author_sort	MAULIN INGGRAINI
title	Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria
title_short	Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria
title_full	Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria
title_fullStr	Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria
title_full_unstemmed	Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria
title_sort	antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/fbb6357f78a34355a0332870b9ee1989
work_keys_str_mv	AT maulininggraini antimicrobialsusceptibilityandmolecularspeciesidentificationofclinicalcarbapenemresistantbacteria AT sitinurfajriah antimicrobialsusceptibilityandmolecularspeciesidentificationofclinicalcarbapenemresistantbacteria AT jepriagungpriyanto antimicrobialsusceptibilityandmolecularspeciesidentificationofclinicalcarbapenemresistantbacteria AT noorandryanilsan antimicrobialsusceptibilityandmolecularspeciesidentificationofclinicalcarbapenemresistantbacteria
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Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria

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