Symbiotic Bacterium-Derived Organic Acids Protect <italic toggle="yes">Delia antiqua</italic> Larvae from Entomopathogenic Fungal Infection

ABSTRACT Colonization resistance, i.e., the protective effects of associated microbiota for the animal host against pathogen infection, has been studied widely over the last 100 years. However, few molecules mediating colonization resistance have been identified. In the symbiosis formed by Delia ant...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fangyuan Zhou, Letian Xu, Xiaoqing Wu, Xiaoyan Zhao, Mei Liu, Xinjian Zhang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	colonization resistance insect-microbe symbiosis defensive association mutualism Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fbb74e8c5c0b46eca51d16e9adab22b3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fbb74e8c5c0b46eca51d16e9adab22b3
record_format	dspace
spelling	oai:doaj.org-article:fbb74e8c5c0b46eca51d16e9adab22b32021-12-02T19:47:37ZSymbiotic Bacterium-Derived Organic Acids Protect <italic toggle="yes">Delia antiqua</italic> Larvae from Entomopathogenic Fungal Infection10.1128/mSystems.00778-202379-5077https://doaj.org/article/fbb74e8c5c0b46eca51d16e9adab22b32020-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00778-20https://doaj.org/toc/2379-5077ABSTRACT Colonization resistance, i.e., the protective effects of associated microbiota for the animal host against pathogen infection, has been studied widely over the last 100 years. However, few molecules mediating colonization resistance have been identified. In the symbiosis formed by Delia antiqua and its associated microbes, six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana, providing an ideal model to investigate the chemical mechanism for colonization resistance. Subsequently using this symbiotic system, we first compared effects of the six bacterial species, and one control bacterium (Klebsiella oxytoca) that showed no antifungal effects, on B. bassiana and its infection of D. antiqua. Second, metabolomic profiles of the six bacteria and K. oxytoca were compared to identify candidate metabolites that may prevent infection. Third, the concentrations of candidate metabolites in situ from axenic and nonaxenic larvae were determined. Finally, effects of artificial metabolite cocktails on B. bassiana and its infection of D. antiqua larvae were determined. Results showed that compared to K. oxytoca, the six bacteria produced a metabolite cocktail showing inhibitory effects on conidial germination, mycelial growth of B. bassiana, and fungal infection. Our work revealed novel molecules that mediate colonization resistance, which could help in developing chemical mechanisms of colonization resistance. Moreover, this work may aid in discovery and expansion of new bioactive antibiotics, promoting development of prophylactic and therapeutic approaches for treating infectious diseases. IMPORTANCE The protection of associated microbiota for their animal hosts against pathogen infection has been studied widely over the last 100 years. However, how those microbes protect the animal host remains unclear. In former studies, body surface microbes of one insect, Delia antiqua, protected the insect larvae from infection with the entomopathogen Beauveria bassiana. By comparing the metabolites produced by microbes that protect the insect and microbes that cannot protect the insect, the question of how the microbes protect the insect is answered. It turns out that body surface bacteria produce a metabolite cocktail that inhibits colonization of B. bassiana and consequently protects the insect. This work reveals novel molecules with antifungal activity, which may aid in discovery and expansion of new prophylactic and therapeutic natural chemicals for treating infectious diseases.Fangyuan ZhouLetian XuXiaoqing WuXiaoyan ZhaoMei LiuXinjian ZhangAmerican Society for Microbiologyarticlecolonization resistanceinsect-microbe symbiosisdefensive associationmutualismMicrobiologyQR1-502ENmSystems, Vol 5, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	colonization resistance insect-microbe symbiosis defensive association mutualism Microbiology QR1-502
spellingShingle	colonization resistance insect-microbe symbiosis defensive association mutualism Microbiology QR1-502 Fangyuan Zhou Letian Xu Xiaoqing Wu Xiaoyan Zhao Mei Liu Xinjian Zhang Symbiotic Bacterium-Derived Organic Acids Protect <italic toggle="yes">Delia antiqua</italic> Larvae from Entomopathogenic Fungal Infection
description	ABSTRACT Colonization resistance, i.e., the protective effects of associated microbiota for the animal host against pathogen infection, has been studied widely over the last 100 years. However, few molecules mediating colonization resistance have been identified. In the symbiosis formed by Delia antiqua and its associated microbes, six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana, providing an ideal model to investigate the chemical mechanism for colonization resistance. Subsequently using this symbiotic system, we first compared effects of the six bacterial species, and one control bacterium (Klebsiella oxytoca) that showed no antifungal effects, on B. bassiana and its infection of D. antiqua. Second, metabolomic profiles of the six bacteria and K. oxytoca were compared to identify candidate metabolites that may prevent infection. Third, the concentrations of candidate metabolites in situ from axenic and nonaxenic larvae were determined. Finally, effects of artificial metabolite cocktails on B. bassiana and its infection of D. antiqua larvae were determined. Results showed that compared to K. oxytoca, the six bacteria produced a metabolite cocktail showing inhibitory effects on conidial germination, mycelial growth of B. bassiana, and fungal infection. Our work revealed novel molecules that mediate colonization resistance, which could help in developing chemical mechanisms of colonization resistance. Moreover, this work may aid in discovery and expansion of new bioactive antibiotics, promoting development of prophylactic and therapeutic approaches for treating infectious diseases. IMPORTANCE The protection of associated microbiota for their animal hosts against pathogen infection has been studied widely over the last 100 years. However, how those microbes protect the animal host remains unclear. In former studies, body surface microbes of one insect, Delia antiqua, protected the insect larvae from infection with the entomopathogen Beauveria bassiana. By comparing the metabolites produced by microbes that protect the insect and microbes that cannot protect the insect, the question of how the microbes protect the insect is answered. It turns out that body surface bacteria produce a metabolite cocktail that inhibits colonization of B. bassiana and consequently protects the insect. This work reveals novel molecules with antifungal activity, which may aid in discovery and expansion of new prophylactic and therapeutic natural chemicals for treating infectious diseases.
format	article
author	Fangyuan Zhou Letian Xu Xiaoqing Wu Xiaoyan Zhao Mei Liu Xinjian Zhang
author_facet	Fangyuan Zhou Letian Xu Xiaoqing Wu Xiaoyan Zhao Mei Liu Xinjian Zhang
author_sort	Fangyuan Zhou
title	Symbiotic Bacterium-Derived Organic Acids Protect <italic toggle="yes">Delia antiqua</italic> Larvae from Entomopathogenic Fungal Infection
title_short	Symbiotic Bacterium-Derived Organic Acids Protect <italic toggle="yes">Delia antiqua</italic> Larvae from Entomopathogenic Fungal Infection
title_full	Symbiotic Bacterium-Derived Organic Acids Protect <italic toggle="yes">Delia antiqua</italic> Larvae from Entomopathogenic Fungal Infection
title_fullStr	Symbiotic Bacterium-Derived Organic Acids Protect <italic toggle="yes">Delia antiqua</italic> Larvae from Entomopathogenic Fungal Infection
title_full_unstemmed	Symbiotic Bacterium-Derived Organic Acids Protect <italic toggle="yes">Delia antiqua</italic> Larvae from Entomopathogenic Fungal Infection
title_sort	symbiotic bacterium-derived organic acids protect <italic toggle="yes">delia antiqua</italic> larvae from entomopathogenic fungal infection
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/fbb74e8c5c0b46eca51d16e9adab22b3
work_keys_str_mv	AT fangyuanzhou symbioticbacteriumderivedorganicacidsprotectitalictoggleyesdeliaantiquaitaliclarvaefromentomopathogenicfungalinfection AT letianxu symbioticbacteriumderivedorganicacidsprotectitalictoggleyesdeliaantiquaitaliclarvaefromentomopathogenicfungalinfection AT xiaoqingwu symbioticbacteriumderivedorganicacidsprotectitalictoggleyesdeliaantiquaitaliclarvaefromentomopathogenicfungalinfection AT xiaoyanzhao symbioticbacteriumderivedorganicacidsprotectitalictoggleyesdeliaantiquaitaliclarvaefromentomopathogenicfungalinfection AT meiliu symbioticbacteriumderivedorganicacidsprotectitalictoggleyesdeliaantiquaitaliclarvaefromentomopathogenicfungalinfection AT xinjianzhang symbioticbacteriumderivedorganicacidsprotectitalictoggleyesdeliaantiquaitaliclarvaefromentomopathogenicfungalinfection
_version_	1718376002090958848

Symbiotic Bacterium-Derived Organic Acids Protect <italic toggle="yes">Delia antiqua</italic> Larvae from Entomopathogenic Fungal Infection

Ejemplares similares