Role of FBXW2 in explant cultures of bovine periosteum-derived cells

Abstract Objective Bone regeneration is a potential technique for treating osteoporosis. A previous study reported that F-box and WD-40 domain-containing protein 2 (FBXW2) localized with osteocalcin in bovine periosteum after 5 weeks of explant culture. However, the osteoblastic functions of FBXW2 r...

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Autor principal:	Mari Akiyama
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	FBXW2 Osteocalcin Periosteum Periosteum-derived cells Fluorescent immunostaining Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/fbb869eebd6a46328739eaa3482b0cb3
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spelling	oai:doaj.org-article:fbb869eebd6a46328739eaa3482b0cb32021-11-08T10:58:35ZRole of FBXW2 in explant cultures of bovine periosteum-derived cells10.1186/s13104-021-05825-z1756-0500https://doaj.org/article/fbb869eebd6a46328739eaa3482b0cb32021-11-01T00:00:00Zhttps://doi.org/10.1186/s13104-021-05825-zhttps://doaj.org/toc/1756-0500Abstract Objective Bone regeneration is a potential technique for treating osteoporosis. A previous study reported that F-box and WD-40 domain-containing protein 2 (FBXW2) localized with osteocalcin in bovine periosteum after 5 weeks of explant culture. However, the osteoblastic functions of FBXW2 remain unclear. In this study, double-fluorescent immunostaining was used to investigate the potential role of FBXW2 and its relationship with osteocalcin. Results At day 0, FBXW2 was expressed in the cambium layer between the bone and periosteum, while osteocalcin was expressed in bone. After explant culture, changes in the periosteum were observed from weeks 1 to 7. At week 1, partial FBXW2 expression was seen with a small amount of osteocalcin. At week 2, a layer of FBXW2 was observed. From weeks 3 to 7, tube-like structures of FBXW and osteocalcin were observed, and periosteum-derived cells were released from the periosteum in areas where no FBXW2 was observed. Bovine periosteum-derived cells can form a three-dimensional cell pellet, because multilayered cell sheets are formed inside of the periosteum in vitro. It is shown that in results FBXW2 is produced in periosteal explants near sites where initial osteogenic activity is observed, suggesting that it may be involved in periosteal osteogenesis.Mari AkiyamaBMCarticleFBXW2OsteocalcinPeriosteumPeriosteum-derived cellsFluorescent immunostainingMedicineRBiology (General)QH301-705.5Science (General)Q1-390ENBMC Research Notes, Vol 14, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	FBXW2 Osteocalcin Periosteum Periosteum-derived cells Fluorescent immunostaining Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
spellingShingle	FBXW2 Osteocalcin Periosteum Periosteum-derived cells Fluorescent immunostaining Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 Mari Akiyama Role of FBXW2 in explant cultures of bovine periosteum-derived cells
description	Abstract Objective Bone regeneration is a potential technique for treating osteoporosis. A previous study reported that F-box and WD-40 domain-containing protein 2 (FBXW2) localized with osteocalcin in bovine periosteum after 5 weeks of explant culture. However, the osteoblastic functions of FBXW2 remain unclear. In this study, double-fluorescent immunostaining was used to investigate the potential role of FBXW2 and its relationship with osteocalcin. Results At day 0, FBXW2 was expressed in the cambium layer between the bone and periosteum, while osteocalcin was expressed in bone. After explant culture, changes in the periosteum were observed from weeks 1 to 7. At week 1, partial FBXW2 expression was seen with a small amount of osteocalcin. At week 2, a layer of FBXW2 was observed. From weeks 3 to 7, tube-like structures of FBXW and osteocalcin were observed, and periosteum-derived cells were released from the periosteum in areas where no FBXW2 was observed. Bovine periosteum-derived cells can form a three-dimensional cell pellet, because multilayered cell sheets are formed inside of the periosteum in vitro. It is shown that in results FBXW2 is produced in periosteal explants near sites where initial osteogenic activity is observed, suggesting that it may be involved in periosteal osteogenesis.
format	article
author	Mari Akiyama
author_facet	Mari Akiyama
author_sort	Mari Akiyama
title	Role of FBXW2 in explant cultures of bovine periosteum-derived cells
title_short	Role of FBXW2 in explant cultures of bovine periosteum-derived cells
title_full	Role of FBXW2 in explant cultures of bovine periosteum-derived cells
title_fullStr	Role of FBXW2 in explant cultures of bovine periosteum-derived cells
title_full_unstemmed	Role of FBXW2 in explant cultures of bovine periosteum-derived cells
title_sort	role of fbxw2 in explant cultures of bovine periosteum-derived cells
publisher	BMC
publishDate	2021
url	https://doaj.org/article/fbb869eebd6a46328739eaa3482b0cb3
work_keys_str_mv	AT mariakiyama roleoffbxw2inexplantculturesofbovineperiosteumderivedcells
_version_	1718442487769464832

Role of FBXW2 in explant cultures of bovine periosteum-derived cells

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