Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles

Katarzyna Pala,1,2 Anna Serwotka,1 Filip Jeleń,1 Piotr Jakimowicz,1 Jacek Otlewski1,2 1Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland; 2Wroclaw Research Centre EIT+, Wroclaw, Poland Abstract: Targeted therapy is a method owing to its limited sid...

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Autores principales:	Pala K, Serwotka A, Jeleń F, Jakimowicz P, Otlewski J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fbc0104cb1a44d83bf7ab8707290022e
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spelling	oai:doaj.org-article:fbc0104cb1a44d83bf7ab8707290022e2021-12-02T07:21:22ZTumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles1178-2013https://doaj.org/article/fbc0104cb1a44d83bf7ab8707290022e2013-12-01T00:00:00Zhttp://www.dovepress.com/tumor-specific-hyperthermia-with-aptamer-tagged-superparamagnetic-nano-a15315https://doaj.org/toc/1178-2013 Katarzyna Pala,1,2 Anna Serwotka,1 Filip Jeleń,1 Piotr Jakimowicz,1 Jacek Otlewski1,2 1Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland; 2Wroclaw Research Centre EIT+, Wroclaw, Poland Abstract: Targeted therapy is a method owing to its limited side effect profile, particularly in cancer treatment. Magnetic hyperthermia, which is induced by nanoparticles (NPs) conjugated with targeting agents, can be useful in combination with chemo- or radiotherapy. In this paper, we constructed dextran-coated ferric oxide NPs conjugated with specific anti-human epidermal growth factor receptor (HER2) aptamer and used them to induce magnetic hyperthermia in cultured cells. The specificity of the tagged NPs was determined by studying their effect relative to that of non-tagged NPs against two cell lines: human adenocarcinoma SK-BR3, overexpressing the HER2 receptor; and U-87 MG, a human glioblastoma epithelial cell line, not expressing HER2. In order to confirm the interaction of the tagged NPs with the cells we used, fluorescence microscopy and fluorescence-activated cell sorting analysis were performed. All of these experiments showed that the aptamer-tagged NPs were highly specific toward the HER2-expressing cells. In addition, a ninetyfold lower dose of the tagged NPs relative to that of the non-tagged NPs was needed to achieve ~50% cell killing by hyperthermia of the SK-BR3 cell line, while for the U-87 MG cells the viability level was close to 100%. These results show that targeted NPs can be applied at substantially lower doses than non-targeted ones to achieve similar effects of hyperthermia, which should greatly limit the side effects of treatment. Keywords: superparamagnetic nanoparticles, hyperthermia, aptamer, targeted therapyPala KSerwotka AJeleń FJakimowicz POtlewski JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 67-76 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Pala K Serwotka A Jeleń F Jakimowicz P Otlewski J Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles
description	Katarzyna Pala,1,2 Anna Serwotka,1 Filip Jeleń,1 Piotr Jakimowicz,1 Jacek Otlewski1,2 1Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland; 2Wroclaw Research Centre EIT+, Wroclaw, Poland Abstract: Targeted therapy is a method owing to its limited side effect profile, particularly in cancer treatment. Magnetic hyperthermia, which is induced by nanoparticles (NPs) conjugated with targeting agents, can be useful in combination with chemo- or radiotherapy. In this paper, we constructed dextran-coated ferric oxide NPs conjugated with specific anti-human epidermal growth factor receptor (HER2) aptamer and used them to induce magnetic hyperthermia in cultured cells. The specificity of the tagged NPs was determined by studying their effect relative to that of non-tagged NPs against two cell lines: human adenocarcinoma SK-BR3, overexpressing the HER2 receptor; and U-87 MG, a human glioblastoma epithelial cell line, not expressing HER2. In order to confirm the interaction of the tagged NPs with the cells we used, fluorescence microscopy and fluorescence-activated cell sorting analysis were performed. All of these experiments showed that the aptamer-tagged NPs were highly specific toward the HER2-expressing cells. In addition, a ninetyfold lower dose of the tagged NPs relative to that of the non-tagged NPs was needed to achieve ~50% cell killing by hyperthermia of the SK-BR3 cell line, while for the U-87 MG cells the viability level was close to 100%. These results show that targeted NPs can be applied at substantially lower doses than non-targeted ones to achieve similar effects of hyperthermia, which should greatly limit the side effects of treatment. Keywords: superparamagnetic nanoparticles, hyperthermia, aptamer, targeted therapy
format	article
author	Pala K Serwotka A Jeleń F Jakimowicz P Otlewski J
author_facet	Pala K Serwotka A Jeleń F Jakimowicz P Otlewski J
author_sort	Pala K
title	Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles
title_short	Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles
title_full	Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles
title_fullStr	Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles
title_full_unstemmed	Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles
title_sort	tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/fbc0104cb1a44d83bf7ab8707290022e
work_keys_str_mv	AT palak tumorspecifichyperthermiawithaptamertaggedsuperparamagneticnanoparticles AT serwotkaa tumorspecifichyperthermiawithaptamertaggedsuperparamagneticnanoparticles AT jelenf tumorspecifichyperthermiawithaptamertaggedsuperparamagneticnanoparticles AT jakimowiczp tumorspecifichyperthermiawithaptamertaggedsuperparamagneticnanoparticles AT otlewskij tumorspecifichyperthermiawithaptamertaggedsuperparamagneticnanoparticles
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Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles

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