Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions

Traction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape defo...

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Bibliographic Details
Main Authors: Daan Vorselen, Yifan Wang, Miguel M. de Jesus, Pavak K. Shah, Matthew J. Footer, Morgan Huse, Wei Cai, Julie A. Theriot
Format: article
Language:EN
Published: Nature Portfolio 2020
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Online Access:https://doaj.org/article/fbc3f6afb17f46f49e6de46d02f86e78
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Summary:Traction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape deformation.