Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions

Traction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape defo...

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Auteurs principaux: Daan Vorselen, Yifan Wang, Miguel M. de Jesus, Pavak K. Shah, Matthew J. Footer, Morgan Huse, Wei Cai, Julie A. Theriot
Format: article
Langue:EN
Publié: Nature Portfolio 2020
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Accès en ligne:https://doaj.org/article/fbc3f6afb17f46f49e6de46d02f86e78
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Résumé:Traction force microscopy is an effective method for measuring cellular forces but it is limited by planar geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape deformation.