Sequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration

Abstract The clinical use of bioactive molecules in bone regeneration has been known to have side effects, which result from uncontrolled and supraphysiological doses. In this study, we demonstrated the synergistic effect of two bioactive molecules, bone morphogenic protein-2 (BMP-2) and alendronate...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dongtak Lee, Maierdanjiang Wufuer, Insu Kim, Tae Hyun Choi, Byung Jun Kim, Hyo Gi Jung, Byoungjun Jeon, Gyudo Lee, Ok Hee Jeon, Hak Chang, Dae Sung Yoon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fbcb851d97a242b7bc43e09f893dc079
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fbcb851d97a242b7bc43e09f893dc079
record_format	dspace
spelling	oai:doaj.org-article:fbcb851d97a242b7bc43e09f893dc0792021-12-02T14:12:47ZSequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration10.1038/s41598-020-80608-32045-2322https://doaj.org/article/fbcb851d97a242b7bc43e09f893dc0792021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80608-3https://doaj.org/toc/2045-2322Abstract The clinical use of bioactive molecules in bone regeneration has been known to have side effects, which result from uncontrolled and supraphysiological doses. In this study, we demonstrated the synergistic effect of two bioactive molecules, bone morphogenic protein-2 (BMP-2) and alendronate (ALN), by releasing them in a sequential manner. Collagen-hydroxyapatite composite scaffolds functionalized using BMP-2 are loaded with biodegradable microspheres where ALN is encapsulated. The results indicate an initial release of BMP-2 for a few days, followed by the sequential release of ALN after two weeks. The composite scaffolds significantly increase osteogenic activity owing to the synergistic effect of BMP-2 and ALN. Enhanced bone regeneration was identified at eight weeks post-implantation in the rat 8-mm critical-sized defect. Our findings suggest that the sequential delivery of BMP-2 and ALN from the scaffolds results in a synergistic effect on bone regeneration, which is unprecedented. Therefore, such a system exhibits potential for the application of cell-free tissue engineering.Dongtak LeeMaierdanjiang WufuerInsu KimTae Hyun ChoiByung Jun KimHyo Gi JungByoungjun JeonGyudo LeeOk Hee JeonHak ChangDae Sung YoonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dongtak Lee Maierdanjiang Wufuer Insu Kim Tae Hyun Choi Byung Jun Kim Hyo Gi Jung Byoungjun Jeon Gyudo Lee Ok Hee Jeon Hak Chang Dae Sung Yoon Sequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration
description	Abstract The clinical use of bioactive molecules in bone regeneration has been known to have side effects, which result from uncontrolled and supraphysiological doses. In this study, we demonstrated the synergistic effect of two bioactive molecules, bone morphogenic protein-2 (BMP-2) and alendronate (ALN), by releasing them in a sequential manner. Collagen-hydroxyapatite composite scaffolds functionalized using BMP-2 are loaded with biodegradable microspheres where ALN is encapsulated. The results indicate an initial release of BMP-2 for a few days, followed by the sequential release of ALN after two weeks. The composite scaffolds significantly increase osteogenic activity owing to the synergistic effect of BMP-2 and ALN. Enhanced bone regeneration was identified at eight weeks post-implantation in the rat 8-mm critical-sized defect. Our findings suggest that the sequential delivery of BMP-2 and ALN from the scaffolds results in a synergistic effect on bone regeneration, which is unprecedented. Therefore, such a system exhibits potential for the application of cell-free tissue engineering.
format	article
author	Dongtak Lee Maierdanjiang Wufuer Insu Kim Tae Hyun Choi Byung Jun Kim Hyo Gi Jung Byoungjun Jeon Gyudo Lee Ok Hee Jeon Hak Chang Dae Sung Yoon
author_facet	Dongtak Lee Maierdanjiang Wufuer Insu Kim Tae Hyun Choi Byung Jun Kim Hyo Gi Jung Byoungjun Jeon Gyudo Lee Ok Hee Jeon Hak Chang Dae Sung Yoon
author_sort	Dongtak Lee
title	Sequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration
title_short	Sequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration
title_full	Sequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration
title_fullStr	Sequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration
title_full_unstemmed	Sequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration
title_sort	sequential dual-drug delivery of bmp-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fbcb851d97a242b7bc43e09f893dc079
work_keys_str_mv	AT dongtaklee sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT maierdanjiangwufuer sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT insukim sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT taehyunchoi sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT byungjunkim sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT hyogijung sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT byoungjunjeon sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT gyudolee sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT okheejeon sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT hakchang sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration AT daesungyoon sequentialdualdrugdeliveryofbmp2andalendronatefromhydroxyapatitecollagenscaffoldsforenhancedboneregeneration
_version_	1718391761539170304

Sequential dual-drug delivery of BMP-2 and alendronate from hydroxyapatite-collagen scaffolds for enhanced bone regeneration

Ejemplares similares