TRPC3 Is Dispensable for β-Alanine Triggered Acute Itch

Abstract The detection of pruritic (itchy) stimuli is mediated by a variety of receptors and channels expressed by primary sensory neurons. The G protein-coupled receptor (GPCR) MRGPRD is selectively expressed by a subset of mouse non-peptidergic nociceptors and functions as the molecular receptor f...

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Autores principales:	Peter Dong, Changxiong Guo, Shengxiang Huang, Minghong Ma, Qin Liu, Wenqin Luo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fbcdfcb688ef425f986d911b12c966f4
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spelling	oai:doaj.org-article:fbcdfcb688ef425f986d911b12c966f42021-12-02T11:53:03ZTRPC3 Is Dispensable for β-Alanine Triggered Acute Itch10.1038/s41598-017-12770-02045-2322https://doaj.org/article/fbcdfcb688ef425f986d911b12c966f42017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-12770-0https://doaj.org/toc/2045-2322Abstract The detection of pruritic (itchy) stimuli is mediated by a variety of receptors and channels expressed by primary sensory neurons. The G protein-coupled receptor (GPCR) MRGPRD is selectively expressed by a subset of mouse non-peptidergic nociceptors and functions as the molecular receptor for the itch-inducing chemical β-alanine. However, the channels responsible for generating electrical signals downstream of MRGPRD remain unclear. Here, we found that a member of the canonical TRP channel family, TRPC3, is highly expressed in MRGPRD+ non-peptidergic nociceptors, raising the possibility of whether TRPC3 functions as a downstream channel in the MRGPRD signaling pathway. We tested TrpC3 null mice for β-alanine induced itch, and found that these mice exhibit normal responses to β-alanine. At the cellular level, calcium influx triggered by β-alanine is also unchanged in cultured DRG neurons from TrpC3 null mice compared to wild type. Together, our results demonstrate that mouse TrpC3 is dispensable for β-alanine-induced acute itch.Peter DongChangxiong GuoShengxiang HuangMinghong MaQin LiuWenqin LuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peter Dong Changxiong Guo Shengxiang Huang Minghong Ma Qin Liu Wenqin Luo TRPC3 Is Dispensable for β-Alanine Triggered Acute Itch
description	Abstract The detection of pruritic (itchy) stimuli is mediated by a variety of receptors and channels expressed by primary sensory neurons. The G protein-coupled receptor (GPCR) MRGPRD is selectively expressed by a subset of mouse non-peptidergic nociceptors and functions as the molecular receptor for the itch-inducing chemical β-alanine. However, the channels responsible for generating electrical signals downstream of MRGPRD remain unclear. Here, we found that a member of the canonical TRP channel family, TRPC3, is highly expressed in MRGPRD+ non-peptidergic nociceptors, raising the possibility of whether TRPC3 functions as a downstream channel in the MRGPRD signaling pathway. We tested TrpC3 null mice for β-alanine induced itch, and found that these mice exhibit normal responses to β-alanine. At the cellular level, calcium influx triggered by β-alanine is also unchanged in cultured DRG neurons from TrpC3 null mice compared to wild type. Together, our results demonstrate that mouse TrpC3 is dispensable for β-alanine-induced acute itch.
format	article
author	Peter Dong Changxiong Guo Shengxiang Huang Minghong Ma Qin Liu Wenqin Luo
author_facet	Peter Dong Changxiong Guo Shengxiang Huang Minghong Ma Qin Liu Wenqin Luo
author_sort	Peter Dong
title	TRPC3 Is Dispensable for β-Alanine Triggered Acute Itch
title_short	TRPC3 Is Dispensable for β-Alanine Triggered Acute Itch
title_full	TRPC3 Is Dispensable for β-Alanine Triggered Acute Itch
title_fullStr	TRPC3 Is Dispensable for β-Alanine Triggered Acute Itch
title_full_unstemmed	TRPC3 Is Dispensable for β-Alanine Triggered Acute Itch
title_sort	trpc3 is dispensable for β-alanine triggered acute itch
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fbcdfcb688ef425f986d911b12c966f4
work_keys_str_mv	AT peterdong trpc3isdispensableforbalaninetriggeredacuteitch AT changxiongguo trpc3isdispensableforbalaninetriggeredacuteitch AT shengxianghuang trpc3isdispensableforbalaninetriggeredacuteitch AT minghongma trpc3isdispensableforbalaninetriggeredacuteitch AT qinliu trpc3isdispensableforbalaninetriggeredacuteitch AT wenqinluo trpc3isdispensableforbalaninetriggeredacuteitch
_version_	1718394904472715264

TRPC3 Is Dispensable for β-Alanine Triggered Acute Itch

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