Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons

Abstract We developed a mouse line targeting midbrain dopamine neurons for Translating Ribosome Affinity Purification(TRAP). Here, we briefly report on the basic characterization of this mouse line including confirmation of expression of the transgene in midbrain dopamine neurons and validation of i...

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Autor principal:	Joseph D. Dougherty
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fbce8b3ebbca499e96adeb76b5e7dce9
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spelling	oai:doaj.org-article:fbce8b3ebbca499e96adeb76b5e7dce92021-12-02T11:41:12ZGeneration and characterization of a mouse line for monitoring translation in dopaminergic neurons10.1038/s41598-017-08618-22045-2322https://doaj.org/article/fbce8b3ebbca499e96adeb76b5e7dce92017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08618-2https://doaj.org/toc/2045-2322Abstract We developed a mouse line targeting midbrain dopamine neurons for Translating Ribosome Affinity Purification(TRAP). Here, we briefly report on the basic characterization of this mouse line including confirmation of expression of the transgene in midbrain dopamine neurons and validation of its effectiveness in capturing mRNA from these cells. We also report a translational profile of these neurons which may be of use to investigators studying the gene expression of these cells. Finally, we have provided the line to Jackson Laboratories for distribution and use in future studies.Joseph D. DoughertyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joseph D. Dougherty Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons
description	Abstract We developed a mouse line targeting midbrain dopamine neurons for Translating Ribosome Affinity Purification(TRAP). Here, we briefly report on the basic characterization of this mouse line including confirmation of expression of the transgene in midbrain dopamine neurons and validation of its effectiveness in capturing mRNA from these cells. We also report a translational profile of these neurons which may be of use to investigators studying the gene expression of these cells. Finally, we have provided the line to Jackson Laboratories for distribution and use in future studies.
format	article
author	Joseph D. Dougherty
author_facet	Joseph D. Dougherty
author_sort	Joseph D. Dougherty
title	Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons
title_short	Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons
title_full	Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons
title_fullStr	Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons
title_full_unstemmed	Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons
title_sort	generation and characterization of a mouse line for monitoring translation in dopaminergic neurons
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fbce8b3ebbca499e96adeb76b5e7dce9
work_keys_str_mv	AT josephddougherty generationandcharacterizationofamouselineformonitoringtranslationindopaminergicneurons
_version_	1718395498086268928

Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons

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