Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.

Exosomes are nano-vesicles secreted by a wide range of mammalian cell types. These vesicles are abundant in serum and other extracellular fluids and contain a large repertoire of proteins, mRNA and microRNA. Exosomes have been implicated in cell to cell communication, the transfer of infectious agen...

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Autores principales:	Rainelli B Koumangoye, Amos M Sakwe, J Shawn Goodwin, Tina Patel, Josiah Ochieng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fbcfb285e45f429fa884d87c51fe8282
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spelling	oai:doaj.org-article:fbcfb285e45f429fa884d87c51fe82822021-11-18T06:46:39ZDetachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.1932-620310.1371/journal.pone.0024234https://doaj.org/article/fbcfb285e45f429fa884d87c51fe82822011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21915303/?tool=EBIhttps://doaj.org/toc/1932-6203Exosomes are nano-vesicles secreted by a wide range of mammalian cell types. These vesicles are abundant in serum and other extracellular fluids and contain a large repertoire of proteins, mRNA and microRNA. Exosomes have been implicated in cell to cell communication, the transfer of infectious agents, and neurodegenerative diseases as well as tumor progression. However, the precise mechanisms by which they are internalized and/or secreted remain poorly understood. In order to follow their release and uptake in breast tumor cells in real time, cell-derived exosomes were tagged with green fluorescent protein (GFP)-CD63 while human serum exosomes were rhodamine isothiocynate-labeled. We show that detachment of adherent cells from various substrata induces a rapid and substantial secretion of exosomes, which then concentrate on the cell surfaces and mediate adhesion to various extracellular matrix proteins. We also demonstrate that disruption of lipid rafts with methyl-beta-cyclodextrin (MβCD) inhibits the internalization of exosomes and that annexins are essential for the exosomal uptake mechanisms. Taken together, these data suggest that cellular detachment is accompanied by significant release of exosomes while cellular adhesion and spreading are enhanced by rapid uptake and disposition of exosomes on the cell surface.Rainelli B KoumangoyeAmos M SakweJ Shawn GoodwinTina PatelJosiah OchiengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24234 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rainelli B Koumangoye Amos M Sakwe J Shawn Goodwin Tina Patel Josiah Ochieng Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.
description	Exosomes are nano-vesicles secreted by a wide range of mammalian cell types. These vesicles are abundant in serum and other extracellular fluids and contain a large repertoire of proteins, mRNA and microRNA. Exosomes have been implicated in cell to cell communication, the transfer of infectious agents, and neurodegenerative diseases as well as tumor progression. However, the precise mechanisms by which they are internalized and/or secreted remain poorly understood. In order to follow their release and uptake in breast tumor cells in real time, cell-derived exosomes were tagged with green fluorescent protein (GFP)-CD63 while human serum exosomes were rhodamine isothiocynate-labeled. We show that detachment of adherent cells from various substrata induces a rapid and substantial secretion of exosomes, which then concentrate on the cell surfaces and mediate adhesion to various extracellular matrix proteins. We also demonstrate that disruption of lipid rafts with methyl-beta-cyclodextrin (MβCD) inhibits the internalization of exosomes and that annexins are essential for the exosomal uptake mechanisms. Taken together, these data suggest that cellular detachment is accompanied by significant release of exosomes while cellular adhesion and spreading are enhanced by rapid uptake and disposition of exosomes on the cell surface.
format	article
author	Rainelli B Koumangoye Amos M Sakwe J Shawn Goodwin Tina Patel Josiah Ochieng
author_facet	Rainelli B Koumangoye Amos M Sakwe J Shawn Goodwin Tina Patel Josiah Ochieng
author_sort	Rainelli B Koumangoye
title	Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.
title_short	Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.
title_full	Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.
title_fullStr	Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.
title_full_unstemmed	Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.
title_sort	detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/fbcfb285e45f429fa884d87c51fe8282
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Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading.

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