Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion

Objective: To investigate the neuroprotective effects of trihydroxyethyl rutin in rats with cervical spinal cord hemi-contusion.Methods: Adult male Sprague–Dawley rats were subjected to hemi-contusion at a stroke depth of 1.2 mm, and then intraperitoneally injected with 50 or 100 mg/kg trihydroxyeth...

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Autores principales:	Yapu Liu, Qi Liu, Zhou Yang, Rong Li, Zhiping Huang, Zucheng Huang, Junhao Liu, Xiuhua Wu, Junyu Lin, Xiaoliang Wu, Qingan Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	trihydroxyethyl rutin neuroprotection spinal cord injury microvascular electrophysiology behavior Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/fbd11ef305a64464883cc9092441996b
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spelling	oai:doaj.org-article:fbd11ef305a64464883cc9092441996b2021-11-18T09:41:30ZTrihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion1662-453X10.3389/fnins.2021.759325https://doaj.org/article/fbd11ef305a64464883cc9092441996b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnins.2021.759325/fullhttps://doaj.org/toc/1662-453XObjective: To investigate the neuroprotective effects of trihydroxyethyl rutin in rats with cervical spinal cord hemi-contusion.Methods: Adult male Sprague–Dawley rats were subjected to hemi-contusion at a stroke depth of 1.2 mm, and then intraperitoneally injected with 50 or 100 mg/kg trihydroxyethyl rutin per day for 12 weeks (T50 and T100 groups, respectively). Changes in somatosensory evoked potentials (SEPs), motor evoked potentials (MEPs), and behavior were continuously monitored. At 12 weeks post-injury, immunohistochemical staining was performed to assess changes in cervical spinal cord microvascular morphology. Magnetic resonance imaging (MRI) scans were performed to examine end-stage injury in the cervical spinal cord, and Eriochrome cyanine-stained slices of spinal cord tissue were evaluated for injury.Results: There were no significant differences in biomechanical parameters among the spinal cord injury, T50 and T100 rat groups. At 3 days-post-injury, there was a significant decrease in grip strength. At 12 weeks post-injury, grip strength recovery was significantly better in the T50 and T100 groups than in the injury group. Compared with the injury group, the total limb placement frequency was significantly higher in the T50 group at 2, 4, 6, 10, and 12 weeks post-injury and in the T100 group at 2, 6, 8, and 10 weeks post-injury. Ipsilateral SEPs and MEPs were dynamic, increasing in latency and decreasing in amplitude in the injury compared with sham group. MRI scanning demonstrated that the coronal, sagittal, and transversal lesion areas were smaller in the T50 and T100 groups than in the injury group. Microvascular density showed a greater reduction in the injury group compared with the T50 and T100 groups. Eriochrome cyanine staining showed that the ipsilateral side, residual parenchyma, and gray matter areas were larger in the T50 and T100 groups than in the injury group.Conclusion: Trihydroxyethyl rutin exhibits robust neuroprotective effects, improving limb motor function and nerve electrophysiological parameters after spinal cord injury, maintaining microvascular density, and reducing the area of injury and degree of demyelination.Yapu LiuYapu LiuQi LiuZhou YangRong LiZhiping HuangZucheng HuangJunhao LiuXiuhua WuJunyu LinXiaoliang WuQingan ZhuFrontiers Media S.A.articletrihydroxyethyl rutinneuroprotectionspinal cord injurymicrovascularelectrophysiologybehaviorNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	trihydroxyethyl rutin neuroprotection spinal cord injury microvascular electrophysiology behavior Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	trihydroxyethyl rutin neuroprotection spinal cord injury microvascular electrophysiology behavior Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Yapu Liu Yapu Liu Qi Liu Zhou Yang Rong Li Zhiping Huang Zucheng Huang Junhao Liu Xiuhua Wu Junyu Lin Xiaoliang Wu Qingan Zhu Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion
description	Objective: To investigate the neuroprotective effects of trihydroxyethyl rutin in rats with cervical spinal cord hemi-contusion.Methods: Adult male Sprague–Dawley rats were subjected to hemi-contusion at a stroke depth of 1.2 mm, and then intraperitoneally injected with 50 or 100 mg/kg trihydroxyethyl rutin per day for 12 weeks (T50 and T100 groups, respectively). Changes in somatosensory evoked potentials (SEPs), motor evoked potentials (MEPs), and behavior were continuously monitored. At 12 weeks post-injury, immunohistochemical staining was performed to assess changes in cervical spinal cord microvascular morphology. Magnetic resonance imaging (MRI) scans were performed to examine end-stage injury in the cervical spinal cord, and Eriochrome cyanine-stained slices of spinal cord tissue were evaluated for injury.Results: There were no significant differences in biomechanical parameters among the spinal cord injury, T50 and T100 rat groups. At 3 days-post-injury, there was a significant decrease in grip strength. At 12 weeks post-injury, grip strength recovery was significantly better in the T50 and T100 groups than in the injury group. Compared with the injury group, the total limb placement frequency was significantly higher in the T50 group at 2, 4, 6, 10, and 12 weeks post-injury and in the T100 group at 2, 6, 8, and 10 weeks post-injury. Ipsilateral SEPs and MEPs were dynamic, increasing in latency and decreasing in amplitude in the injury compared with sham group. MRI scanning demonstrated that the coronal, sagittal, and transversal lesion areas were smaller in the T50 and T100 groups than in the injury group. Microvascular density showed a greater reduction in the injury group compared with the T50 and T100 groups. Eriochrome cyanine staining showed that the ipsilateral side, residual parenchyma, and gray matter areas were larger in the T50 and T100 groups than in the injury group.Conclusion: Trihydroxyethyl rutin exhibits robust neuroprotective effects, improving limb motor function and nerve electrophysiological parameters after spinal cord injury, maintaining microvascular density, and reducing the area of injury and degree of demyelination.
format	article
author	Yapu Liu Yapu Liu Qi Liu Zhou Yang Rong Li Zhiping Huang Zucheng Huang Junhao Liu Xiuhua Wu Junyu Lin Xiaoliang Wu Qingan Zhu
author_facet	Yapu Liu Yapu Liu Qi Liu Zhou Yang Rong Li Zhiping Huang Zucheng Huang Junhao Liu Xiuhua Wu Junyu Lin Xiaoliang Wu Qingan Zhu
author_sort	Yapu Liu
title	Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion
title_short	Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion
title_full	Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion
title_fullStr	Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion
title_full_unstemmed	Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion
title_sort	trihydroxyethyl rutin provides neuroprotection in rats with cervical spinal cord hemi-contusion
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fbd11ef305a64464883cc9092441996b
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Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion

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