Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA

Zhenyu Shao,1 Jingyu Shao,2 Bingxu Tan,1 Shanghui Guan,1 Zhulong Liu,1 Zengjun Zhao,1 Fangfang He,1 Jian Zhao3 1Department of Radiotherapy, Cancer Centre, Qilu Hospital, Shandong University, Ji’nan, Shandong, People’s Republic of China; 2Affiliated Hospital of Northwest Institu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shao Z, Shao J, Tan B, Guan S, Liu Z, Zhao Z, He F, Zhao J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fbd4b4cf122942939484ac58d8f8a4aa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fbd4b4cf122942939484ac58d8f8a4aa
record_format	dspace
spelling	oai:doaj.org-article:fbd4b4cf122942939484ac58d8f8a4aa2021-12-02T07:22:53ZTargeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA1178-2013https://doaj.org/article/fbd4b4cf122942939484ac58d8f8a4aa2015-02-01T00:00:00Zhttp://www.dovepress.com/targeted-lung-cancer-therapy-preparation-and-optimization-of-transferr-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Zhenyu Shao,1 Jingyu Shao,2 Bingxu Tan,1 Shanghui Guan,1 Zhulong Liu,1 Zengjun Zhao,1 Fangfang He,1 Jian Zhao3 1Department of Radiotherapy, Cancer Centre, Qilu Hospital, Shandong University, Ji’nan, Shandong, People’s Republic of China; 2Affiliated Hospital of Northwest Institute of Mechanical and Electrical Engineering, Xianyang, Shaanxi, People’s Republic of China; 3Department of Thoracic Surgery, Qilu Hospital, Shandong University, Ji’nan, Shandong, People’s Republic of China Purpose: Nanostructured lipid carriers (NLC) represent an improved generation of lipid nanoparticles. They have specific nanostructures to accommodate drugs/genes, and thus achieve higher loading capacity. The aim of this study was to develop transferrin (Tf)-decorated NLC as multifunctional nanomedicine for co-delivery of paclitaxel (PTX) and enhanced green fluorescence protein plasmid.Methods: Firstly, Tf-conjugated ligands were synthesized. Secondly, PTX- and DNA-loaded NLC (PTX-DNA-NLC) was prepared. Finally, Tf-containing ligands were used for the surface decoration of NLC. Their average size, zeta potential, drug, and gene loading were evaluated. Human non-small cell lung carcinoma cell line (NCl-H460 cells) was used for the testing of in vitro transfection efficiency, and in vivo transfection efficiency of NLC was evaluated on mice bearing NCl-H460 cells.Results: Tf-decorated PTX and DNA co-encapsulated NLC (Tf-PTX-DNA-NLC) were nano-sized particles with positive zeta potential. Tf-PTX-DNA-NLC displayed low cytotoxicity, high gene transfection efficiency, and enhanced antitumor activity in vitro and in vivo.Conclusion: The results demonstrated that Tf-PTX-DNA-NLC can achieve impressive antitumor activity and gene transfection efficiency. Tf decoration also enhanced the active targeting ability of the carriers to NCl-H460 cells. The novel drug and gene delivery system offers a promising strategy for the treatment of lung cancer. Keywords: co-delivery, nanostructured lipid carriers, transferrin decoration, active targeting systems, paclitaxelShao ZShao JTan BGuan SLiu ZZhao ZHe FZhao JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1223-1233 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Shao Z Shao J Tan B Guan S Liu Z Zhao Z He F Zhao J Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA
description	Zhenyu Shao,1 Jingyu Shao,2 Bingxu Tan,1 Shanghui Guan,1 Zhulong Liu,1 Zengjun Zhao,1 Fangfang He,1 Jian Zhao3 1Department of Radiotherapy, Cancer Centre, Qilu Hospital, Shandong University, Ji’nan, Shandong, People’s Republic of China; 2Affiliated Hospital of Northwest Institute of Mechanical and Electrical Engineering, Xianyang, Shaanxi, People’s Republic of China; 3Department of Thoracic Surgery, Qilu Hospital, Shandong University, Ji’nan, Shandong, People’s Republic of China Purpose: Nanostructured lipid carriers (NLC) represent an improved generation of lipid nanoparticles. They have specific nanostructures to accommodate drugs/genes, and thus achieve higher loading capacity. The aim of this study was to develop transferrin (Tf)-decorated NLC as multifunctional nanomedicine for co-delivery of paclitaxel (PTX) and enhanced green fluorescence protein plasmid.Methods: Firstly, Tf-conjugated ligands were synthesized. Secondly, PTX- and DNA-loaded NLC (PTX-DNA-NLC) was prepared. Finally, Tf-containing ligands were used for the surface decoration of NLC. Their average size, zeta potential, drug, and gene loading were evaluated. Human non-small cell lung carcinoma cell line (NCl-H460 cells) was used for the testing of in vitro transfection efficiency, and in vivo transfection efficiency of NLC was evaluated on mice bearing NCl-H460 cells.Results: Tf-decorated PTX and DNA co-encapsulated NLC (Tf-PTX-DNA-NLC) were nano-sized particles with positive zeta potential. Tf-PTX-DNA-NLC displayed low cytotoxicity, high gene transfection efficiency, and enhanced antitumor activity in vitro and in vivo.Conclusion: The results demonstrated that Tf-PTX-DNA-NLC can achieve impressive antitumor activity and gene transfection efficiency. Tf decoration also enhanced the active targeting ability of the carriers to NCl-H460 cells. The novel drug and gene delivery system offers a promising strategy for the treatment of lung cancer. Keywords: co-delivery, nanostructured lipid carriers, transferrin decoration, active targeting systems, paclitaxel
format	article
author	Shao Z Shao J Tan B Guan S Liu Z Zhao Z He F Zhao J
author_facet	Shao Z Shao J Tan B Guan S Liu Z Zhao Z He F Zhao J
author_sort	Shao Z
title	Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA
title_short	Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA
title_full	Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA
title_fullStr	Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA
title_full_unstemmed	Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA
title_sort	targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and dna
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/fbd4b4cf122942939484ac58d8f8a4aa
work_keys_str_mv	AT shaoz targetedlungcancertherapypreparationandoptimizationoftransferrindecoratednanostructuredlipidcarriersasnovelnanomedicineforcodeliveryofanticancerdrugsanddna AT shaoj targetedlungcancertherapypreparationandoptimizationoftransferrindecoratednanostructuredlipidcarriersasnovelnanomedicineforcodeliveryofanticancerdrugsanddna AT tanb targetedlungcancertherapypreparationandoptimizationoftransferrindecoratednanostructuredlipidcarriersasnovelnanomedicineforcodeliveryofanticancerdrugsanddna AT guans targetedlungcancertherapypreparationandoptimizationoftransferrindecoratednanostructuredlipidcarriersasnovelnanomedicineforcodeliveryofanticancerdrugsanddna AT liuz targetedlungcancertherapypreparationandoptimizationoftransferrindecoratednanostructuredlipidcarriersasnovelnanomedicineforcodeliveryofanticancerdrugsanddna AT zhaoz targetedlungcancertherapypreparationandoptimizationoftransferrindecoratednanostructuredlipidcarriersasnovelnanomedicineforcodeliveryofanticancerdrugsanddna AT hef targetedlungcancertherapypreparationandoptimizationoftransferrindecoratednanostructuredlipidcarriersasnovelnanomedicineforcodeliveryofanticancerdrugsanddna AT zhaoj targetedlungcancertherapypreparationandoptimizationoftransferrindecoratednanostructuredlipidcarriersasnovelnanomedicineforcodeliveryofanticancerdrugsanddna
_version_	1718399495346061312

Targeted lung cancer therapy: preparation and optimization of transferrin-decorated nanostructured lipid carriers as novel nanomedicine for co-delivery of anticancer drugs and DNA

Ejemplares similares