COMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS

The gut microbiota of healthy people, living in different regions, may vary considerably. The article presents the results of comparative qualitative and quantitative study of the intestine aerobic and anaerobic microbiota of residents of the Republic of Guinea (46) and Russia (60). The content of m...

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Autores principales:	A. Yu. Popova, L. A. Kaftyreva, L. V. Suzhaeva, E. V. Voitenkova, A. V. Zabrovskaia, S. A. Egorova, M. A. Makarova, Z. N. Matveeva, E. V. Zueva, A. A. Porin, M. Y. Boiro, O. K. Konstantinov, A. A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2018
Materias:	intestine microbiome republic of guinea enterobacteriaceae hafnia alvei comamonas kerstersii staphylococcus aureus escherichia сoli enterococcus spp. Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/fbd740f3c82540eaa6e391024fa8d504
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spelling	oai:doaj.org-article:fbd740f3c82540eaa6e391024fa8d5042021-11-22T07:09:48ZCOMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS2220-76192313-739810.15789/2220-7619-2017-4-375-382https://doaj.org/article/fbd740f3c82540eaa6e391024fa8d5042018-01-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/595https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The gut microbiota of healthy people, living in different regions, may vary considerably. The article presents the results of comparative qualitative and quantitative study of the intestine aerobic and anaerobic microbiota of residents of the Republic of Guinea (46) and Russia (60). The content of microorganisms (Enterobacteriaceae, Enterococcus spp., Lactobacillus spp., Bifidobacterium spp., Staphylococcus spp., Candida spp., non-fermentative gram-negative bacteria and others) in 1.0 gram of faeces was determined by bacteriological methods. Generic and species identification was performed using Vitek 2 Compact (bioMerieux, France), tube biochemical tests and MALDI-TОF mass spectrometry (Bruker Daltonics, Germany). Pearson χ2 criterion, p < 0.05 and Fisher’s exact test (medstatistica.ru) were used to assess the differences in the compared groups. 95% confidence intervals were calculated by the method of Wilson. Microbiological disorders were characterized by a decrease in the number of obligate microorganisms, an increase in the number of facultative (opportunistic) microorganisms (above 106 CFU/g) and the emergence of their associations. Dysbiotic disorders were identified in both compared groups: in 100% patients from Republic of Guinea (95% CI:92.3–100) and in 86.7% patients from St. Petersburg (95% CI:75.8–93.1). Severe degree of microbiota disorders in the residents of the Republic of Guinea was revealed at 19.6% (95% CI:10.7–33.2), in group of residents of St. Petersburg in 9.6% (95% CI:4.2–20.6). In both groups the microbiota disorders of third degree were detected less frequently, compared with the microbiota disorders of second degree. The study has found no significant differences in the content of obligate bacteria (Bifidobacterium spp. and Lactobacillus spp.), however, significant differences in the species composition of the facultative part of the microbiota were revealed. The residents of the Republic of Guinea had “atypical” E. coli (lactosonegative and hemolytic) and non-fermenting gram-negative bacteria Comamonas kerstersii more frequently. Opportunistic microorganism associations have been found in 30.4% of the residents of the Republic of Guinea (95% CI:19.1–44.8) and 18.3% of residents of St. Petersburg (95% CI:10.6–29.9). Opportunistic microorganism associations from the residents of the Republic of Guinea always contained Staphylococcus aureus. It is necessary to conduct further research on a bigger population to access the differences in the compared groups in Staphylococcus aureus and Hafnia alvei.A. Yu. PopovaL. A. KaftyrevaL. V. SuzhaevaE. V. VoitenkovaA. V. ZabrovskaiaS. A. EgorovaM. A. MakarovaZ. N. MatveevaE. V. ZuevaA. A. PorinM. Y. BoiroO. K. KonstantinovA. A. TotolianSankt-Peterburg : NIIÈM imeni Pasteraarticleintestine microbiomerepublic of guineaenterobacteriaceaehafnia alveicomamonas kerstersiistaphylococcus aureusescherichia сolienterococcus spp.Infectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 7, Iss 4, Pp 375-382 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	intestine microbiome republic of guinea enterobacteriaceae hafnia alvei comamonas kerstersii staphylococcus aureus escherichia сoli enterococcus spp. Infectious and parasitic diseases RC109-216
spellingShingle	intestine microbiome republic of guinea enterobacteriaceae hafnia alvei comamonas kerstersii staphylococcus aureus escherichia сoli enterococcus spp. Infectious and parasitic diseases RC109-216 A. Yu. Popova L. A. Kaftyreva L. V. Suzhaeva E. V. Voitenkova A. V. Zabrovskaia S. A. Egorova M. A. Makarova Z. N. Matveeva E. V. Zueva A. A. Porin M. Y. Boiro O. K. Konstantinov A. A. Totolian COMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS
description	The gut microbiota of healthy people, living in different regions, may vary considerably. The article presents the results of comparative qualitative and quantitative study of the intestine aerobic and anaerobic microbiota of residents of the Republic of Guinea (46) and Russia (60). The content of microorganisms (Enterobacteriaceae, Enterococcus spp., Lactobacillus spp., Bifidobacterium spp., Staphylococcus spp., Candida spp., non-fermentative gram-negative bacteria and others) in 1.0 gram of faeces was determined by bacteriological methods. Generic and species identification was performed using Vitek 2 Compact (bioMerieux, France), tube biochemical tests and MALDI-TОF mass spectrometry (Bruker Daltonics, Germany). Pearson χ2 criterion, p < 0.05 and Fisher’s exact test (medstatistica.ru) were used to assess the differences in the compared groups. 95% confidence intervals were calculated by the method of Wilson. Microbiological disorders were characterized by a decrease in the number of obligate microorganisms, an increase in the number of facultative (opportunistic) microorganisms (above 106 CFU/g) and the emergence of their associations. Dysbiotic disorders were identified in both compared groups: in 100% patients from Republic of Guinea (95% CI:92.3–100) and in 86.7% patients from St. Petersburg (95% CI:75.8–93.1). Severe degree of microbiota disorders in the residents of the Republic of Guinea was revealed at 19.6% (95% CI:10.7–33.2), in group of residents of St. Petersburg in 9.6% (95% CI:4.2–20.6). In both groups the microbiota disorders of third degree were detected less frequently, compared with the microbiota disorders of second degree. The study has found no significant differences in the content of obligate bacteria (Bifidobacterium spp. and Lactobacillus spp.), however, significant differences in the species composition of the facultative part of the microbiota were revealed. The residents of the Republic of Guinea had “atypical” E. coli (lactosonegative and hemolytic) and non-fermenting gram-negative bacteria Comamonas kerstersii more frequently. Opportunistic microorganism associations have been found in 30.4% of the residents of the Republic of Guinea (95% CI:19.1–44.8) and 18.3% of residents of St. Petersburg (95% CI:10.6–29.9). Opportunistic microorganism associations from the residents of the Republic of Guinea always contained Staphylococcus aureus. It is necessary to conduct further research on a bigger population to access the differences in the compared groups in Staphylococcus aureus and Hafnia alvei.
format	article
author	A. Yu. Popova L. A. Kaftyreva L. V. Suzhaeva E. V. Voitenkova A. V. Zabrovskaia S. A. Egorova M. A. Makarova Z. N. Matveeva E. V. Zueva A. A. Porin M. Y. Boiro O. K. Konstantinov A. A. Totolian
author_facet	A. Yu. Popova L. A. Kaftyreva L. V. Suzhaeva E. V. Voitenkova A. V. Zabrovskaia S. A. Egorova M. A. Makarova Z. N. Matveeva E. V. Zueva A. A. Porin M. Y. Boiro O. K. Konstantinov A. A. Totolian
author_sort	A. Yu. Popova
title	COMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS
title_short	COMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS
title_full	COMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS
title_fullStr	COMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS
title_full_unstemmed	COMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS
title_sort	comparative characteristics of intestine microbiome of republic of guinea and russian federation residents
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2018
url	https://doaj.org/article/fbd740f3c82540eaa6e391024fa8d504
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COMPARATIVE CHARACTERISTICS OF INTESTINE MICROBIOME OF REPUBLIC OF GUINEA AND RUSSIAN FEDERATION RESIDENTS

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