MicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation

Abstract Unilateral vestibular deafferentation (UVD) interrupts afferent signals from one side, resulting in an imbalance of the resting activity between bilateral vestibular nuclei. Vestibular compensation is the process of balancing the resting activity to reestablish homeostasis. Here, we investi...

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Autores principales:	Mun Young Chang, Sohyeon Park, Jun Jae Choi, Young-Kook Kim, Myung-Whan Suh, Jun Ho Lee, Seung Ha Oh, Moo Kyun Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fbdabd3955914c408c2e1759b61e2d25
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spelling	oai:doaj.org-article:fbdabd3955914c408c2e1759b61e2d252021-12-02T11:40:59ZMicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation10.1038/s41598-017-09422-82045-2322https://doaj.org/article/fbdabd3955914c408c2e1759b61e2d252017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09422-8https://doaj.org/toc/2045-2322Abstract Unilateral vestibular deafferentation (UVD) interrupts afferent signals from one side, resulting in an imbalance of the resting activity between bilateral vestibular nuclei. Vestibular compensation is the process of balancing the resting activity to reestablish homeostasis. Here, we investigated microRNAs (miRNAs) that regulate vestibular compensation using the Sprague–Dawley rat. After determining the progression of vestibular compensation following UVD, microarray analysis was performed and nine miRNAs were selected as candidates. Following validation by quantitative reverse transcription-PCR, three miRNAs remained. We assessed the effect of these miRNAs on vestibular compensation using miRNA oligomers. We compared the results of the rotarod test and 5-bromo-2′-deoxyuridine immunohistochemistry following UVD between the control group and the groups in which the candidate miRNA oligomers were administered. Administration of miR-218a-5p, 219a-5p, and 221-3p oligomers significantly affected vestibular compensation. Target pathway analysis of these miRNAs supported our results. Our findings suggest that the miRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation.Mun Young ChangSohyeon ParkJun Jae ChoiYoung-Kook KimMyung-Whan SuhJun Ho LeeSeung Ha OhMoo Kyun ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mun Young Chang Sohyeon Park Jun Jae Choi Young-Kook Kim Myung-Whan Suh Jun Ho Lee Seung Ha Oh Moo Kyun Park MicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation
description	Abstract Unilateral vestibular deafferentation (UVD) interrupts afferent signals from one side, resulting in an imbalance of the resting activity between bilateral vestibular nuclei. Vestibular compensation is the process of balancing the resting activity to reestablish homeostasis. Here, we investigated microRNAs (miRNAs) that regulate vestibular compensation using the Sprague–Dawley rat. After determining the progression of vestibular compensation following UVD, microarray analysis was performed and nine miRNAs were selected as candidates. Following validation by quantitative reverse transcription-PCR, three miRNAs remained. We assessed the effect of these miRNAs on vestibular compensation using miRNA oligomers. We compared the results of the rotarod test and 5-bromo-2′-deoxyuridine immunohistochemistry following UVD between the control group and the groups in which the candidate miRNA oligomers were administered. Administration of miR-218a-5p, 219a-5p, and 221-3p oligomers significantly affected vestibular compensation. Target pathway analysis of these miRNAs supported our results. Our findings suggest that the miRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation.
format	article
author	Mun Young Chang Sohyeon Park Jun Jae Choi Young-Kook Kim Myung-Whan Suh Jun Ho Lee Seung Ha Oh Moo Kyun Park
author_facet	Mun Young Chang Sohyeon Park Jun Jae Choi Young-Kook Kim Myung-Whan Suh Jun Ho Lee Seung Ha Oh Moo Kyun Park
author_sort	Mun Young Chang
title	MicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation
title_short	MicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation
title_full	MicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation
title_fullStr	MicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation
title_full_unstemmed	MicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation
title_sort	micrornas 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fbdabd3955914c408c2e1759b61e2d25
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MicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation

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