A transgenic female killing system for the genetic control of Drosophila suzukii

Abstract The spotted wing Drosophila (Drosophila suzukii) is an invasive pest of soft-skinned fruit crops. It is rapidly transmitted in Europe and North America, causing widespread agricultural losses. Genetic control strategies such as the sterile insect technique (SIT) have been proposed as enviro...

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Autores principales:	Marc F. Schetelig, Jonas Schwirz, Ying Yan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fbdf4c76bb6c4603b66fec4e55ead0d1
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spelling	oai:doaj.org-article:fbdf4c76bb6c4603b66fec4e55ead0d12021-12-02T17:14:30ZA transgenic female killing system for the genetic control of Drosophila suzukii10.1038/s41598-021-91938-12045-2322https://doaj.org/article/fbdf4c76bb6c4603b66fec4e55ead0d12021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91938-1https://doaj.org/toc/2045-2322Abstract The spotted wing Drosophila (Drosophila suzukii) is an invasive pest of soft-skinned fruit crops. It is rapidly transmitted in Europe and North America, causing widespread agricultural losses. Genetic control strategies such as the sterile insect technique (SIT) have been proposed as environment-friendly and species-restricted approaches for this pest. However, females are inefficient agents in SIT programs. Here we report a conditional female-killing (FK) strategy based on the tetracycline-off system. We assembled sixteen genetic constructs for testing in vitro and in vivo. Twenty-four independent transgenic strains of D. suzukii were generated and tested for female-specific lethality. The strongest FK effect in the absence of tetracycline was achieved by the construct containing D. suzukii nullo promoter for early gene expression, D. suzukii pro-apoptotic gene hid Ala4 for lethality, and the transformer gene intron from the Mediterranean fruit fly Ceratitis capitata for female-specific splicing. One strain carrying this construct eliminated 100% of the female offspring during embryogenesis and produced only males. However, homozygous females from these FK strains were not viable on a tetracycline-supplemented diet, possibly due to the basal expression of hid Ala4 . Potential improvements to the gene constructs and the use of such FK strains in an SIT program are discussed.Marc F. ScheteligJonas SchwirzYing YanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marc F. Schetelig Jonas Schwirz Ying Yan A transgenic female killing system for the genetic control of Drosophila suzukii
description	Abstract The spotted wing Drosophila (Drosophila suzukii) is an invasive pest of soft-skinned fruit crops. It is rapidly transmitted in Europe and North America, causing widespread agricultural losses. Genetic control strategies such as the sterile insect technique (SIT) have been proposed as environment-friendly and species-restricted approaches for this pest. However, females are inefficient agents in SIT programs. Here we report a conditional female-killing (FK) strategy based on the tetracycline-off system. We assembled sixteen genetic constructs for testing in vitro and in vivo. Twenty-four independent transgenic strains of D. suzukii were generated and tested for female-specific lethality. The strongest FK effect in the absence of tetracycline was achieved by the construct containing D. suzukii nullo promoter for early gene expression, D. suzukii pro-apoptotic gene hid Ala4 for lethality, and the transformer gene intron from the Mediterranean fruit fly Ceratitis capitata for female-specific splicing. One strain carrying this construct eliminated 100% of the female offspring during embryogenesis and produced only males. However, homozygous females from these FK strains were not viable on a tetracycline-supplemented diet, possibly due to the basal expression of hid Ala4 . Potential improvements to the gene constructs and the use of such FK strains in an SIT program are discussed.
format	article
author	Marc F. Schetelig Jonas Schwirz Ying Yan
author_facet	Marc F. Schetelig Jonas Schwirz Ying Yan
author_sort	Marc F. Schetelig
title	A transgenic female killing system for the genetic control of Drosophila suzukii
title_short	A transgenic female killing system for the genetic control of Drosophila suzukii
title_full	A transgenic female killing system for the genetic control of Drosophila suzukii
title_fullStr	A transgenic female killing system for the genetic control of Drosophila suzukii
title_full_unstemmed	A transgenic female killing system for the genetic control of Drosophila suzukii
title_sort	transgenic female killing system for the genetic control of drosophila suzukii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fbdf4c76bb6c4603b66fec4e55ead0d1
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A transgenic female killing system for the genetic control of Drosophila suzukii

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