A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of Salmonella typhimurium in Milk

Salmonella typhimurium (S. typhimurium) is a foodborne pathogen that has caused numerous outbreaks worldwide, necessitating the development of on-site strategy to prevent early contamination. Here, we set up an enzyme-free strategy for aptamer-catalyzed hairpin...

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Autores principales:	Sihan Chen, Xinran Zong, Jiapeng Zheng, Jiaxin Zhang, Mengyao Zhou, Qing Chen, Chaoxin Man, Yujun Jiang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Salmonella typhimurium</i> catalytic hairpin assembly AuNPs on-site detection aptamer Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/fbe09687ed9747f1b327850290efed60
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spelling	oai:doaj.org-article:fbe09687ed9747f1b327850290efed602021-11-25T17:32:21ZA Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of <i>Salmonella typhimurium</i> in Milk10.3390/foods101125392304-8158https://doaj.org/article/fbe09687ed9747f1b327850290efed602021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2539https://doaj.org/toc/2304-8158<i>Salmonella typhimurium</i> (<i>S. typhimurium</i>) is a foodborne pathogen that has caused numerous outbreaks worldwide, necessitating the development of on-site strategy to prevent early contamination. Here, we set up an enzyme-free strategy for aptamer-catalyzed hairpin assembly in which salt-induced aggregation of unmodified gold nanoparticles (AuNPs) served as a colorimetric signal output, allowing on-site detection of <i>S. typhimurium</i> in milk. The aptamer-functionalized magnetic beads were used as a vehicle of specifically enriching target bacteria which conjugated with target aptamer to trigger the “Y” shape catalytic hairpin assembly (Y-CHA) circuit. Due to the hairpins desorbing from the surface of AuNPs to the formation of a large amount of double-stranded DNA (dsDNA), AuNPs turned from dispersion to aggregation in the presence of <i>S. typhimurium</i>, resulting in a change of the colorimetric signal from red to blue-gray. The signal output showed a linear relationship for <i>S. typhimurium</i> over a concentration range of 10<sup>2</sup> to 10<sup>6</sup> CFU/mL, with a sensitivity of 2.4 × 10<sup>2</sup> CFU/mL under optimal conditions. The visual protocol has excellent selectivity even in the presence of other competitive bacteria and has been validated in real milk samples with a sensitivity of 2.8 × 10<sup>3</sup> CFU/mL.Sihan ChenXinran ZongJiapeng ZhengJiaxin ZhangMengyao ZhouQing ChenChaoxin ManYujun JiangMDPI AGarticle<i>Salmonella typhimurium</i>catalytic hairpin assemblyAuNPson-site detectionaptamerChemical technologyTP1-1185ENFoods, Vol 10, Iss 2539, p 2539 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Salmonella typhimurium</i> catalytic hairpin assembly AuNPs on-site detection aptamer Chemical technology TP1-1185
spellingShingle	<i>Salmonella typhimurium</i> catalytic hairpin assembly AuNPs on-site detection aptamer Chemical technology TP1-1185 Sihan Chen Xinran Zong Jiapeng Zheng Jiaxin Zhang Mengyao Zhou Qing Chen Chaoxin Man Yujun Jiang A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of <i>Salmonella typhimurium</i> in Milk
description	<i>Salmonella typhimurium</i> (<i>S. typhimurium</i>) is a foodborne pathogen that has caused numerous outbreaks worldwide, necessitating the development of on-site strategy to prevent early contamination. Here, we set up an enzyme-free strategy for aptamer-catalyzed hairpin assembly in which salt-induced aggregation of unmodified gold nanoparticles (AuNPs) served as a colorimetric signal output, allowing on-site detection of <i>S. typhimurium</i> in milk. The aptamer-functionalized magnetic beads were used as a vehicle of specifically enriching target bacteria which conjugated with target aptamer to trigger the “Y” shape catalytic hairpin assembly (Y-CHA) circuit. Due to the hairpins desorbing from the surface of AuNPs to the formation of a large amount of double-stranded DNA (dsDNA), AuNPs turned from dispersion to aggregation in the presence of <i>S. typhimurium</i>, resulting in a change of the colorimetric signal from red to blue-gray. The signal output showed a linear relationship for <i>S. typhimurium</i> over a concentration range of 10<sup>2</sup> to 10<sup>6</sup> CFU/mL, with a sensitivity of 2.4 × 10<sup>2</sup> CFU/mL under optimal conditions. The visual protocol has excellent selectivity even in the presence of other competitive bacteria and has been validated in real milk samples with a sensitivity of 2.8 × 10<sup>3</sup> CFU/mL.
format	article
author	Sihan Chen Xinran Zong Jiapeng Zheng Jiaxin Zhang Mengyao Zhou Qing Chen Chaoxin Man Yujun Jiang
author_facet	Sihan Chen Xinran Zong Jiapeng Zheng Jiaxin Zhang Mengyao Zhou Qing Chen Chaoxin Man Yujun Jiang
author_sort	Sihan Chen
title	A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of <i>Salmonella typhimurium</i> in Milk
title_short	A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of <i>Salmonella typhimurium</i> in Milk
title_full	A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of <i>Salmonella typhimurium</i> in Milk
title_fullStr	A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of <i>Salmonella typhimurium</i> in Milk
title_full_unstemmed	A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of <i>Salmonella typhimurium</i> in Milk
title_sort	colorimetric strategy based on aptamer-catalyzed hairpin assembly for the on-site detection of <i>salmonella typhimurium</i> in milk
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fbe09687ed9747f1b327850290efed60
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A Colorimetric Strategy Based on Aptamer-Catalyzed Hairpin Assembly for the On-Site Detection of <i>Salmonella typhimurium</i> in Milk

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