Bee Venom Prevents Mucin 5AC Production through Inhibition of AKT and SPDEF Activation in Airway Epithelia Cells

IL-13 induces mucus metaplasia, which causes airway obstruction in asthma. Bee venom (BV) and its components have shown anti-inflammatory effects in allergic diseases such as atopic dermatitis and asthma. In this study, we investigated the effect of BV on IL-13-induced mucus metaplasia through activ...

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Autores principales:	Sanga Kim, Hee-Won Kim, Seok-Hwan Chang, Kang-Hyun Leem, Hae-Jeong Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bee venom asthma mucus metaplasia interleukin-13 mucin 5AC Medicine R
Acceso en línea:	https://doaj.org/article/fbe5686fa5584e8f84e3841ea3419382
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spelling	oai:doaj.org-article:fbe5686fa5584e8f84e3841ea34193822021-11-25T19:08:43ZBee Venom Prevents Mucin 5AC Production through Inhibition of AKT and SPDEF Activation in Airway Epithelia Cells10.3390/toxins131107732072-6651https://doaj.org/article/fbe5686fa5584e8f84e3841ea34193822021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/773https://doaj.org/toc/2072-6651IL-13 induces mucus metaplasia, which causes airway obstruction in asthma. Bee venom (BV) and its components have shown anti-inflammatory effects in allergic diseases such as atopic dermatitis and asthma. In this study, we investigated the effect of BV on IL-13-induced mucus metaplasia through activation of the signal transducer and activator of transcription (STAT6), and regulation of SAM-pointed domain containing Ets-like factor (SPDEF) and forkhead box A2 (FOXA2) in the airway epithelia cell line A549. In A549 cells, BV (1.0 µg/mL) inhibited IL-13 (10 ng/mL)-induced AKT phosphorylation, increase in SPDEF protein expression, and decrease in FOXA2 protein expression—but not STAT6 phosphorylation. BV also prevented the IL-13-induced increase in mucin 5AC (MUC5AC) mRNA and protein expression. Moreover, we observed that inhibition of phosphoinositide 3 kinase (PI3K)/AKT using LY294002 (50 µM) could reverse the alterations in FOXA2 and MUC5AC expression -by IL-13 and BV. However, LY294002 did not affect IL-13- and BV-induced changes in SPDEF expression. These findings indicate that BV inhibits MUC5AC production through the regulation of SPDEF and FOXA2. The inhibition of MUC5AC production through FOXA2 is mediated via the suppression of PI3K/AKT activation by BV. BV may be helpful in the prevention of mucus metaplasia in asthma.Sanga KimHee-Won KimSeok-Hwan ChangKang-Hyun LeemHae-Jeong ParkMDPI AGarticlebee venomasthmamucus metaplasiainterleukin-13mucin 5ACMedicineRENToxins, Vol 13, Iss 773, p 773 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bee venom asthma mucus metaplasia interleukin-13 mucin 5AC Medicine R
spellingShingle	bee venom asthma mucus metaplasia interleukin-13 mucin 5AC Medicine R Sanga Kim Hee-Won Kim Seok-Hwan Chang Kang-Hyun Leem Hae-Jeong Park Bee Venom Prevents Mucin 5AC Production through Inhibition of AKT and SPDEF Activation in Airway Epithelia Cells
description	IL-13 induces mucus metaplasia, which causes airway obstruction in asthma. Bee venom (BV) and its components have shown anti-inflammatory effects in allergic diseases such as atopic dermatitis and asthma. In this study, we investigated the effect of BV on IL-13-induced mucus metaplasia through activation of the signal transducer and activator of transcription (STAT6), and regulation of SAM-pointed domain containing Ets-like factor (SPDEF) and forkhead box A2 (FOXA2) in the airway epithelia cell line A549. In A549 cells, BV (1.0 µg/mL) inhibited IL-13 (10 ng/mL)-induced AKT phosphorylation, increase in SPDEF protein expression, and decrease in FOXA2 protein expression—but not STAT6 phosphorylation. BV also prevented the IL-13-induced increase in mucin 5AC (MUC5AC) mRNA and protein expression. Moreover, we observed that inhibition of phosphoinositide 3 kinase (PI3K)/AKT using LY294002 (50 µM) could reverse the alterations in FOXA2 and MUC5AC expression -by IL-13 and BV. However, LY294002 did not affect IL-13- and BV-induced changes in SPDEF expression. These findings indicate that BV inhibits MUC5AC production through the regulation of SPDEF and FOXA2. The inhibition of MUC5AC production through FOXA2 is mediated via the suppression of PI3K/AKT activation by BV. BV may be helpful in the prevention of mucus metaplasia in asthma.
format	article
author	Sanga Kim Hee-Won Kim Seok-Hwan Chang Kang-Hyun Leem Hae-Jeong Park
author_facet	Sanga Kim Hee-Won Kim Seok-Hwan Chang Kang-Hyun Leem Hae-Jeong Park
author_sort	Sanga Kim
title	Bee Venom Prevents Mucin 5AC Production through Inhibition of AKT and SPDEF Activation in Airway Epithelia Cells
title_short	Bee Venom Prevents Mucin 5AC Production through Inhibition of AKT and SPDEF Activation in Airway Epithelia Cells
title_full	Bee Venom Prevents Mucin 5AC Production through Inhibition of AKT and SPDEF Activation in Airway Epithelia Cells
title_fullStr	Bee Venom Prevents Mucin 5AC Production through Inhibition of AKT and SPDEF Activation in Airway Epithelia Cells
title_full_unstemmed	Bee Venom Prevents Mucin 5AC Production through Inhibition of AKT and SPDEF Activation in Airway Epithelia Cells
title_sort	bee venom prevents mucin 5ac production through inhibition of akt and spdef activation in airway epithelia cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fbe5686fa5584e8f84e3841ea3419382
work_keys_str_mv	AT sangakim beevenompreventsmucin5acproductionthroughinhibitionofaktandspdefactivationinairwayepitheliacells AT heewonkim beevenompreventsmucin5acproductionthroughinhibitionofaktandspdefactivationinairwayepitheliacells AT seokhwanchang beevenompreventsmucin5acproductionthroughinhibitionofaktandspdefactivationinairwayepitheliacells AT kanghyunleem beevenompreventsmucin5acproductionthroughinhibitionofaktandspdefactivationinairwayepitheliacells AT haejeongpark beevenompreventsmucin5acproductionthroughinhibitionofaktandspdefactivationinairwayepitheliacells
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Bee Venom Prevents Mucin 5AC Production through Inhibition of AKT and SPDEF Activation in Airway Epithelia Cells

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