Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice

Context: We identified an active prenylated derivative of genistein, 8-prenylgenistein (8PG) from Erythrina variegata L. (Leguminosae) and found that 8PG increased osteoprotective effects of genistein in oestrogen-deficient mice. Objective: This study investigated and compared the oestrogenic effect...

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Autores principales:	Xiao-Li Li, Li Sui, Fu-Hui Lin, Yin Lian, Lian-Zhong Ai, Yan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	oestrogen receptor phytoestrogen uterus vagina Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/fbe9b6dae6094669b7cb7fa66cc16f15
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spelling	oai:doaj.org-article:fbe9b6dae6094669b7cb7fa66cc16f152021-11-17T14:21:56ZDifferential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice1388-02091744-511610.1080/13880209.2019.1590422https://doaj.org/article/fbe9b6dae6094669b7cb7fa66cc16f152019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1590422https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: We identified an active prenylated derivative of genistein, 8-prenylgenistein (8PG) from Erythrina variegata L. (Leguminosae) and found that 8PG increased osteoprotective effects of genistein in oestrogen-deficient mice. Objective: This study investigated and compared the oestrogenic effects of genistein and 8PG on uterus and vagina of immature mice. Materials and methods: Immature female CD-1 mice were orally treated with vehicle (Control, n = 10) or genistein (75 mg/kg, n = 10) or 8PG with low (8PG-L, 75 mg/kg, n = 10) and high dose (8PG-H, 150 mg/kg, n = 10) for 7 consecutive days by intragastric gavage. The uterus and vagina were harvested for histological and molecular measurements. Results: Treatment with genistein and 8PG-H significantly increased uterus index (1.98 ± 0.21 & 1.49 ± 0.16 mg/g) and vagina index (3.83 ± 0.11 & 3.13 ± 0.25 mg/g) as compared to untreated control (uterus, 1.12 ± 0.13 mg/g; vagina, 2.32 ± 0.18 mg/g). Accordingly, both genistein and 8PG-H made vaginal cells keratinized and induced uterine and vaginal hypertrophy associated with the endometrial proliferation. 8PG-L did not affect oestrus cycle and histology of uterus and vagina. Treatment of immature mice with genistein or 8PG-H upregulated protein expression of oestrogen receptor-α (ER-α) and proliferating cell nuclear antigen (PCNA), but 8PG-L did not alter ER-α and PCNA expression in uterus and vagina. Conclusion: This study indicated that 8-prenylgenistein exerted oestrogenic effects in immature female mice. The efficacy and safety of 8-prenylgenistein when applied in improving oestrogen deficiency-induced syndrome requires further elucidation.Xiao-Li LiLi SuiFu-Hui LinYin LianLian-Zhong AiYan ZhangTaylor & Francis Grouparticleoestrogen receptorphytoestrogenuterusvaginaTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 226-230 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	oestrogen receptor phytoestrogen uterus vagina Therapeutics. Pharmacology RM1-950
spellingShingle	oestrogen receptor phytoestrogen uterus vagina Therapeutics. Pharmacology RM1-950 Xiao-Li Li Li Sui Fu-Hui Lin Yin Lian Lian-Zhong Ai Yan Zhang Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
description	Context: We identified an active prenylated derivative of genistein, 8-prenylgenistein (8PG) from Erythrina variegata L. (Leguminosae) and found that 8PG increased osteoprotective effects of genistein in oestrogen-deficient mice. Objective: This study investigated and compared the oestrogenic effects of genistein and 8PG on uterus and vagina of immature mice. Materials and methods: Immature female CD-1 mice were orally treated with vehicle (Control, n = 10) or genistein (75 mg/kg, n = 10) or 8PG with low (8PG-L, 75 mg/kg, n = 10) and high dose (8PG-H, 150 mg/kg, n = 10) for 7 consecutive days by intragastric gavage. The uterus and vagina were harvested for histological and molecular measurements. Results: Treatment with genistein and 8PG-H significantly increased uterus index (1.98 ± 0.21 & 1.49 ± 0.16 mg/g) and vagina index (3.83 ± 0.11 & 3.13 ± 0.25 mg/g) as compared to untreated control (uterus, 1.12 ± 0.13 mg/g; vagina, 2.32 ± 0.18 mg/g). Accordingly, both genistein and 8PG-H made vaginal cells keratinized and induced uterine and vaginal hypertrophy associated with the endometrial proliferation. 8PG-L did not affect oestrus cycle and histology of uterus and vagina. Treatment of immature mice with genistein or 8PG-H upregulated protein expression of oestrogen receptor-α (ER-α) and proliferating cell nuclear antigen (PCNA), but 8PG-L did not alter ER-α and PCNA expression in uterus and vagina. Conclusion: This study indicated that 8-prenylgenistein exerted oestrogenic effects in immature female mice. The efficacy and safety of 8-prenylgenistein when applied in improving oestrogen deficiency-induced syndrome requires further elucidation.
format	article
author	Xiao-Li Li Li Sui Fu-Hui Lin Yin Lian Lian-Zhong Ai Yan Zhang
author_facet	Xiao-Li Li Li Sui Fu-Hui Lin Yin Lian Lian-Zhong Ai Yan Zhang
author_sort	Xiao-Li Li
title	Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_short	Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_full	Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_fullStr	Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_full_unstemmed	Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
title_sort	differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/fbe9b6dae6094669b7cb7fa66cc16f15
work_keys_str_mv	AT xiaolili differentialeffectsofgenisteinand8prenylgenisteinonreproductivetissuesinimmaturefemalemice AT lisui differentialeffectsofgenisteinand8prenylgenisteinonreproductivetissuesinimmaturefemalemice AT fuhuilin differentialeffectsofgenisteinand8prenylgenisteinonreproductivetissuesinimmaturefemalemice AT yinlian differentialeffectsofgenisteinand8prenylgenisteinonreproductivetissuesinimmaturefemalemice AT lianzhongai differentialeffectsofgenisteinand8prenylgenisteinonreproductivetissuesinimmaturefemalemice AT yanzhang differentialeffectsofgenisteinand8prenylgenisteinonreproductivetissuesinimmaturefemalemice
_version_	1718425523027181568

Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice

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