Effect of gut microbiota from Henoch-Schönlein Purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats

Abstract Background It has been proven that gut microbiota alterations are involved in the development of Henoch-Schönlein Purpura (HSP). However, the pathogenesis of HSP hasn’t been eluciated. This study was to investigate the impact of gut microbiota from HSP on ASIC3 expression and interactions b...

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Autores principales:	Jin-kun Wang, Bo Yan, Jun-mei Zhao, Li-ping Yuan
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Gut motility Gut microbiota Henoch-Schönlein Purpura Acid-sensing ion channel-3 Pediatrics RJ1-570
Acceso en línea:	https://doaj.org/article/fbeeffade720405f9faee7d91bc6dc2f
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spelling	oai:doaj.org-article:fbeeffade720405f9faee7d91bc6dc2f2021-12-05T12:21:22ZEffect of gut microbiota from Henoch-Schönlein Purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats10.1186/s12887-021-03013-31471-2431https://doaj.org/article/fbeeffade720405f9faee7d91bc6dc2f2021-12-01T00:00:00Zhttps://doi.org/10.1186/s12887-021-03013-3https://doaj.org/toc/1471-2431Abstract Background It has been proven that gut microbiota alterations are involved in the development of Henoch-Schönlein Purpura (HSP). However, the pathogenesis of HSP hasn’t been eluciated. This study was to investigate the impact of gut microbiota from HSP on ASIC3 expression and interactions between microbiota and ASIC3 expression in the development of HSP. Methods Feces collected from HSP and healthy children at the First Affiliated Hospital of Anhui Medical University were made into fecal microbial solutions. Germ-free rats were randomly assigned to either the control or HSP groups. The HSP group of rats were administered the fecal microbiota solution of HSP children, while the control group rats were administered the fecal microbiota solution of healthy children. Abdominal withdrawal reflex (AWR) and intestinal propulsion rate of the rats were used to determine visceral sensitivity. Composition of the gut microbiota of HSP children was determined using 16S rRNA gene sequencing. ASIC3 expression in the colon was ascertained through qRT-PCR as well as western blotting analysis. Results The results showed a reduction in the number of species and abundance in the intestinal microbiota of children with HSP. Visceral sensitivity and intestinal propulsion rate of HSP group rats increased significantly, compared with the control group. Colon ASIC3 mRNA and protein levels in the HSP group were found to be upregulated. The microbiota dysbiosis of HSP patients could stimulate ASIC3 expression in the colon of Germ-free rats, which in turn affected intestinal motility. Conclusions These results suggested that HSP children had intestinal microbiota disorder, which might affect gut motility by down-regulating colon ASIC3 expression in rats.Jin-kun WangBo YanJun-mei ZhaoLi-ping YuanBMCarticleGut motilityGut microbiotaHenoch-Schönlein PurpuraAcid-sensing ion channel-3PediatricsRJ1-570ENBMC Pediatrics, Vol 21, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gut motility Gut microbiota Henoch-Schönlein Purpura Acid-sensing ion channel-3 Pediatrics RJ1-570
spellingShingle	Gut motility Gut microbiota Henoch-Schönlein Purpura Acid-sensing ion channel-3 Pediatrics RJ1-570 Jin-kun Wang Bo Yan Jun-mei Zhao Li-ping Yuan Effect of gut microbiota from Henoch-Schönlein Purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats
description	Abstract Background It has been proven that gut microbiota alterations are involved in the development of Henoch-Schönlein Purpura (HSP). However, the pathogenesis of HSP hasn’t been eluciated. This study was to investigate the impact of gut microbiota from HSP on ASIC3 expression and interactions between microbiota and ASIC3 expression in the development of HSP. Methods Feces collected from HSP and healthy children at the First Affiliated Hospital of Anhui Medical University were made into fecal microbial solutions. Germ-free rats were randomly assigned to either the control or HSP groups. The HSP group of rats were administered the fecal microbiota solution of HSP children, while the control group rats were administered the fecal microbiota solution of healthy children. Abdominal withdrawal reflex (AWR) and intestinal propulsion rate of the rats were used to determine visceral sensitivity. Composition of the gut microbiota of HSP children was determined using 16S rRNA gene sequencing. ASIC3 expression in the colon was ascertained through qRT-PCR as well as western blotting analysis. Results The results showed a reduction in the number of species and abundance in the intestinal microbiota of children with HSP. Visceral sensitivity and intestinal propulsion rate of HSP group rats increased significantly, compared with the control group. Colon ASIC3 mRNA and protein levels in the HSP group were found to be upregulated. The microbiota dysbiosis of HSP patients could stimulate ASIC3 expression in the colon of Germ-free rats, which in turn affected intestinal motility. Conclusions These results suggested that HSP children had intestinal microbiota disorder, which might affect gut motility by down-regulating colon ASIC3 expression in rats.
format	article
author	Jin-kun Wang Bo Yan Jun-mei Zhao Li-ping Yuan
author_facet	Jin-kun Wang Bo Yan Jun-mei Zhao Li-ping Yuan
author_sort	Jin-kun Wang
title	Effect of gut microbiota from Henoch-Schönlein Purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats
title_short	Effect of gut microbiota from Henoch-Schönlein Purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats
title_full	Effect of gut microbiota from Henoch-Schönlein Purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats
title_fullStr	Effect of gut microbiota from Henoch-Schönlein Purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats
title_full_unstemmed	Effect of gut microbiota from Henoch-Schönlein Purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats
title_sort	effect of gut microbiota from henoch-schönlein purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats
publisher	BMC
publishDate	2021
url	https://doaj.org/article/fbeeffade720405f9faee7d91bc6dc2f
work_keys_str_mv	AT jinkunwang effectofgutmicrobiotafromhenochschonleinpurpurapatientsonacidsensitiveionchannel3expressionandintestinalmotilityingermfreerats AT boyan effectofgutmicrobiotafromhenochschonleinpurpurapatientsonacidsensitiveionchannel3expressionandintestinalmotilityingermfreerats AT junmeizhao effectofgutmicrobiotafromhenochschonleinpurpurapatientsonacidsensitiveionchannel3expressionandintestinalmotilityingermfreerats AT lipingyuan effectofgutmicrobiotafromhenochschonleinpurpurapatientsonacidsensitiveionchannel3expressionandintestinalmotilityingermfreerats
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Effect of gut microbiota from Henoch-Schönlein Purpura patients on acid-sensitive ion channel 3 expression and intestinal motility in germ-free rats

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