The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.

Discrete clusters of circadian clock neurons temporally organize daily behaviors such as sleep and wake. In Drosophila, a network of just 150 neurons drives two peaks of timed activity in the morning and evening. A subset of these neurons expresses the neuropeptide pigment dispersing factor (PDF), w...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bridget C Lear, Luoying Zhang, Ravi Allada
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fbf0d8deec8e4d14ad0aad395302c26e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fbf0d8deec8e4d14ad0aad395302c26e
record_format	dspace
spelling	oai:doaj.org-article:fbf0d8deec8e4d14ad0aad395302c26e2021-11-25T05:34:06ZThe neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.1544-91731545-788510.1371/journal.pbio.1000154https://doaj.org/article/fbf0d8deec8e4d14ad0aad395302c26e2009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19621061/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Discrete clusters of circadian clock neurons temporally organize daily behaviors such as sleep and wake. In Drosophila, a network of just 150 neurons drives two peaks of timed activity in the morning and evening. A subset of these neurons expresses the neuropeptide pigment dispersing factor (PDF), which is important for promoting morning behavior as well as maintaining robust free-running rhythmicity in constant conditions. Yet, how PDF acts on downstream circuits to mediate rhythmic behavior is unknown. Using circuit-directed rescue of PDF receptor mutants, we show that PDF targeting of just approximately 30 non-PDF evening circadian neurons is sufficient to drive morning behavior. This function is not accompanied by large changes in core molecular oscillators in light-dark, indicating that PDF RECEPTOR likely regulates the output of these cells under these conditions. We find that PDF also acts on this focused set of non-PDF neurons to regulate both evening activity phase and period length, consistent with modest resetting effects on core oscillators. PDF likely acts on more distributed pacemaker neuron targets, including the PDF neurons themselves, to regulate rhythmic strength. Here we reveal defining features of the circuit-diagram for PDF peptide function in circadian behavior, revealing the direct neuronal targets of PDF as well as its behavioral functions at those sites. These studies define a key direct output circuit sufficient for multiple PDF dependent behaviors.Bridget C LearLuoying ZhangRavi AlladaPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 7, p e1000154 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Bridget C Lear Luoying Zhang Ravi Allada The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.
description	Discrete clusters of circadian clock neurons temporally organize daily behaviors such as sleep and wake. In Drosophila, a network of just 150 neurons drives two peaks of timed activity in the morning and evening. A subset of these neurons expresses the neuropeptide pigment dispersing factor (PDF), which is important for promoting morning behavior as well as maintaining robust free-running rhythmicity in constant conditions. Yet, how PDF acts on downstream circuits to mediate rhythmic behavior is unknown. Using circuit-directed rescue of PDF receptor mutants, we show that PDF targeting of just approximately 30 non-PDF evening circadian neurons is sufficient to drive morning behavior. This function is not accompanied by large changes in core molecular oscillators in light-dark, indicating that PDF RECEPTOR likely regulates the output of these cells under these conditions. We find that PDF also acts on this focused set of non-PDF neurons to regulate both evening activity phase and period length, consistent with modest resetting effects on core oscillators. PDF likely acts on more distributed pacemaker neuron targets, including the PDF neurons themselves, to regulate rhythmic strength. Here we reveal defining features of the circuit-diagram for PDF peptide function in circadian behavior, revealing the direct neuronal targets of PDF as well as its behavioral functions at those sites. These studies define a key direct output circuit sufficient for multiple PDF dependent behaviors.
format	article
author	Bridget C Lear Luoying Zhang Ravi Allada
author_facet	Bridget C Lear Luoying Zhang Ravi Allada
author_sort	Bridget C Lear
title	The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.
title_short	The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.
title_full	The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.
title_fullStr	The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.
title_full_unstemmed	The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.
title_sort	neuropeptide pdf acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/fbf0d8deec8e4d14ad0aad395302c26e
work_keys_str_mv	AT bridgetclear theneuropeptidepdfactsdirectlyoneveningpacemakerneuronstoregulatemultiplefeaturesofcircadianbehavior AT luoyingzhang theneuropeptidepdfactsdirectlyoneveningpacemakerneuronstoregulatemultiplefeaturesofcircadianbehavior AT raviallada theneuropeptidepdfactsdirectlyoneveningpacemakerneuronstoregulatemultiplefeaturesofcircadianbehavior AT bridgetclear neuropeptidepdfactsdirectlyoneveningpacemakerneuronstoregulatemultiplefeaturesofcircadianbehavior AT luoyingzhang neuropeptidepdfactsdirectlyoneveningpacemakerneuronstoregulatemultiplefeaturesofcircadianbehavior AT raviallada neuropeptidepdfactsdirectlyoneveningpacemakerneuronstoregulatemultiplefeaturesofcircadianbehavior
_version_	1718414616704319488

The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.

Ejemplares similares