DNA methylation of Vesicular Glutamate Transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study

Abstract DNA methylation and gene expression can be altered by early life stress (ELS) and/or ethanol consumption. The present study aimed to investigate whether DNA methylation of the Vesicular Glutamate Transporters (Vglut)1-3 is related to previously observed Vglut1-3 transcriptional differences...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maria Vrettou, Liying Yan, Kent W. Nilsson, Åsa Wallén-Mackenzie, Ingrid Nylander, Erika Comasco
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fbff724fc1634f1493ca430f41626888
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fbff724fc1634f1493ca430f41626888
record_format	dspace
spelling	oai:doaj.org-article:fbff724fc1634f1493ca430f416268882021-12-02T16:14:35ZDNA methylation of Vesicular Glutamate Transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study10.1038/s41598-021-94739-82045-2322https://doaj.org/article/fbff724fc1634f1493ca430f416268882021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94739-8https://doaj.org/toc/2045-2322Abstract DNA methylation and gene expression can be altered by early life stress (ELS) and/or ethanol consumption. The present study aimed to investigate whether DNA methylation of the Vesicular Glutamate Transporters (Vglut)1-3 is related to previously observed Vglut1-3 transcriptional differences in the ventral tegmental area (VTA), nucleus accumbens (Acb), dorsal striatum (dStr) and medial prefrontal cortex (mPFC) of adult rats exposed to ELS, modelled by maternal separation, and voluntary ethanol consumption. Targeted next-generation bisulfite sequencing was performed to identify the methylation levels on 61 5′-cytosine-phosphate-guanosine-3′ sites (CpGs) in potential regulatory regions of Vglut1, 53 for Vglut2, and 51 for Vglut3. In the VTA, ELS in ethanol-drinking rats was associated with Vglut1-2 CpG-specific hypomethylation, whereas bidirectional Vglut2 methylation differences at single CpGs were associated with ELS alone. Exposure to both ELS and ethanol, in the Acb, was associated with lower promoter and higher intronic Vglut3 methylation; and in the dStr, with higher and lower methylation in 26% and 43% of the analyzed Vglut1 CpGs, respectively. In the mPFC, lower Vglut2 methylation was observed upon exposure to ELS or ethanol. The present findings suggest Vglut1-3 CpG-specific methylation signatures of ELS and ethanol drinking, underlying previously reported Vglut1-3 transcriptional differences in the mesocorticolimbic brain.Maria VrettouLiying YanKent W. NilssonÅsa Wallén-MackenzieIngrid NylanderErika ComascoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Vrettou Liying Yan Kent W. Nilsson Åsa Wallén-Mackenzie Ingrid Nylander Erika Comasco DNA methylation of Vesicular Glutamate Transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study
description	Abstract DNA methylation and gene expression can be altered by early life stress (ELS) and/or ethanol consumption. The present study aimed to investigate whether DNA methylation of the Vesicular Glutamate Transporters (Vglut)1-3 is related to previously observed Vglut1-3 transcriptional differences in the ventral tegmental area (VTA), nucleus accumbens (Acb), dorsal striatum (dStr) and medial prefrontal cortex (mPFC) of adult rats exposed to ELS, modelled by maternal separation, and voluntary ethanol consumption. Targeted next-generation bisulfite sequencing was performed to identify the methylation levels on 61 5′-cytosine-phosphate-guanosine-3′ sites (CpGs) in potential regulatory regions of Vglut1, 53 for Vglut2, and 51 for Vglut3. In the VTA, ELS in ethanol-drinking rats was associated with Vglut1-2 CpG-specific hypomethylation, whereas bidirectional Vglut2 methylation differences at single CpGs were associated with ELS alone. Exposure to both ELS and ethanol, in the Acb, was associated with lower promoter and higher intronic Vglut3 methylation; and in the dStr, with higher and lower methylation in 26% and 43% of the analyzed Vglut1 CpGs, respectively. In the mPFC, lower Vglut2 methylation was observed upon exposure to ELS or ethanol. The present findings suggest Vglut1-3 CpG-specific methylation signatures of ELS and ethanol drinking, underlying previously reported Vglut1-3 transcriptional differences in the mesocorticolimbic brain.
format	article
author	Maria Vrettou Liying Yan Kent W. Nilsson Åsa Wallén-Mackenzie Ingrid Nylander Erika Comasco
author_facet	Maria Vrettou Liying Yan Kent W. Nilsson Åsa Wallén-Mackenzie Ingrid Nylander Erika Comasco
author_sort	Maria Vrettou
title	DNA methylation of Vesicular Glutamate Transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study
title_short	DNA methylation of Vesicular Glutamate Transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study
title_full	DNA methylation of Vesicular Glutamate Transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study
title_fullStr	DNA methylation of Vesicular Glutamate Transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study
title_full_unstemmed	DNA methylation of Vesicular Glutamate Transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study
title_sort	dna methylation of vesicular glutamate transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fbff724fc1634f1493ca430f41626888
work_keys_str_mv	AT mariavrettou dnamethylationofvesicularglutamatetransportersinthemesocorticolimbicbrainfollowingearlylifestressandadultethanolexposureanexplorativestudy AT liyingyan dnamethylationofvesicularglutamatetransportersinthemesocorticolimbicbrainfollowingearlylifestressandadultethanolexposureanexplorativestudy AT kentwnilsson dnamethylationofvesicularglutamatetransportersinthemesocorticolimbicbrainfollowingearlylifestressandadultethanolexposureanexplorativestudy AT asawallenmackenzie dnamethylationofvesicularglutamatetransportersinthemesocorticolimbicbrainfollowingearlylifestressandadultethanolexposureanexplorativestudy AT ingridnylander dnamethylationofvesicularglutamatetransportersinthemesocorticolimbicbrainfollowingearlylifestressandadultethanolexposureanexplorativestudy AT erikacomasco dnamethylationofvesicularglutamatetransportersinthemesocorticolimbicbrainfollowingearlylifestressandadultethanolexposureanexplorativestudy
_version_	1718384328627453952

DNA methylation of Vesicular Glutamate Transporters in the mesocorticolimbic brain following early-life stress and adult ethanol exposure—an explorative study

Ejemplares similares