3D cyclorama for digital unrolling and visualisation of deformed tubes

Abstract Colonic crypts are tubular glands that multiply through a symmetric branching process called crypt fission. During the early stages of colorectal cancer, the normal fission process is disturbed, leading to asymmetrical branching or budding. The challenging shapes of the budding crypts make...

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Autores principales:	Charalambos Rossides, Sylvia L. F. Pender, Philipp Schneider
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc09a6546c41489eafa4ca19fe318cec
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spelling	oai:doaj.org-article:fc09a6546c41489eafa4ca19fe318cec2021-12-02T16:50:23Z3D cyclorama for digital unrolling and visualisation of deformed tubes10.1038/s41598-021-93184-x2045-2322https://doaj.org/article/fc09a6546c41489eafa4ca19fe318cec2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93184-xhttps://doaj.org/toc/2045-2322Abstract Colonic crypts are tubular glands that multiply through a symmetric branching process called crypt fission. During the early stages of colorectal cancer, the normal fission process is disturbed, leading to asymmetrical branching or budding. The challenging shapes of the budding crypts make it difficult to prepare paraffin sections for conventional histology, resulting in colonic cross sections with crypts that are only partially visible. To study crypt budding in situ and in three dimensions (3D), we employ X-ray micro-computed tomography to image intact colons, and a new method we developed (3D cyclorama) to digitally unroll them. Here, we present, verify and validate our ‘3D cyclorama’ method that digitally unrolls deformed tubes of non-uniform thickness. It employs principles from electrostatics to reform the tube into a series of onion-like surfaces, which are mapped onto planar panoramic views. This enables the study of features extending over several layers of the tube’s depth, demonstrated here by two case studies: (i) microvilli in the human placenta and (ii) 3D-printed adhesive films for drug delivery. Our 3D cyclorama method can provide novel insights into a wide spectrum of applications where digital unrolling or flattening is necessary, including long bones, teeth roots and ancient scrolls.Charalambos RossidesSylvia L. F. PenderPhilipp SchneiderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charalambos Rossides Sylvia L. F. Pender Philipp Schneider 3D cyclorama for digital unrolling and visualisation of deformed tubes
description	Abstract Colonic crypts are tubular glands that multiply through a symmetric branching process called crypt fission. During the early stages of colorectal cancer, the normal fission process is disturbed, leading to asymmetrical branching or budding. The challenging shapes of the budding crypts make it difficult to prepare paraffin sections for conventional histology, resulting in colonic cross sections with crypts that are only partially visible. To study crypt budding in situ and in three dimensions (3D), we employ X-ray micro-computed tomography to image intact colons, and a new method we developed (3D cyclorama) to digitally unroll them. Here, we present, verify and validate our ‘3D cyclorama’ method that digitally unrolls deformed tubes of non-uniform thickness. It employs principles from electrostatics to reform the tube into a series of onion-like surfaces, which are mapped onto planar panoramic views. This enables the study of features extending over several layers of the tube’s depth, demonstrated here by two case studies: (i) microvilli in the human placenta and (ii) 3D-printed adhesive films for drug delivery. Our 3D cyclorama method can provide novel insights into a wide spectrum of applications where digital unrolling or flattening is necessary, including long bones, teeth roots and ancient scrolls.
format	article
author	Charalambos Rossides Sylvia L. F. Pender Philipp Schneider
author_facet	Charalambos Rossides Sylvia L. F. Pender Philipp Schneider
author_sort	Charalambos Rossides
title	3D cyclorama for digital unrolling and visualisation of deformed tubes
title_short	3D cyclorama for digital unrolling and visualisation of deformed tubes
title_full	3D cyclorama for digital unrolling and visualisation of deformed tubes
title_fullStr	3D cyclorama for digital unrolling and visualisation of deformed tubes
title_full_unstemmed	3D cyclorama for digital unrolling and visualisation of deformed tubes
title_sort	3d cyclorama for digital unrolling and visualisation of deformed tubes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fc09a6546c41489eafa4ca19fe318cec
work_keys_str_mv	AT charalambosrossides 3dcycloramafordigitalunrollingandvisualisationofdeformedtubes AT sylvialfpender 3dcycloramafordigitalunrollingandvisualisationofdeformedtubes AT philippschneider 3dcycloramafordigitalunrollingandvisualisationofdeformedtubes
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3D cyclorama for digital unrolling and visualisation of deformed tubes

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