A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency

Abstract Replacement of the in vivo rabies vaccine potency test (NIH test) by in vitro methods had been discussed by several researcher including WHO expert working groups. In this paper, a time-resolved fluoroimmunoassay (TRFIA) for the assay of rabies virus glycoprotein in rabies vaccine was first...

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Autores principales:	Guanfeng Lin, Shaolang Chen, Hui Zhao, Junyu Liang, Qiaoting Deng, Rongliang Liang, Xinxin Guo, Zhenhua Chen, Baihong Chen, Tiancai Liu, Yingsong Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc0a269ebd254638917cd589043832a0
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spelling	oai:doaj.org-article:fc0a269ebd254638917cd589043832a02021-12-02T11:52:27ZA time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency10.1038/s41598-017-07687-72045-2322https://doaj.org/article/fc0a269ebd254638917cd589043832a02017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07687-7https://doaj.org/toc/2045-2322Abstract Replacement of the in vivo rabies vaccine potency test (NIH test) by in vitro methods had been discussed by several researcher including WHO expert working groups. In this paper, a time-resolved fluoroimmunoassay (TRFIA) for the assay of rabies virus glycoprotein in rabies vaccine was first established to estimate the rabies vaccine potency by using specific monoclonal antibody that only recognized the native, trimeric and immunogenic form of rabies virus glycoprotein. Potency of the rabies virus glycoprotein was assayed with satisfactory performance under optimal conditions, and the method demonstrated satisfactory results when applied in practical samples. The correlation coefficient of potency values obtained from the present TRFIA and ELISA was 0.912, and 0.903 for those from the present TRFIA and NIH test. These preliminary results confirmed that this TRFIA can replace ELISA with higher performance, and could be a promising replacement of the NIH test. Based upon these results, the present TRFIA seemed to be a convenient tool for evaluating rabies vaccine potency and its products at different stages accordingly.Guanfeng LinShaolang ChenHui ZhaoJunyu LiangQiaoting DengRongliang LiangXinxin GuoZhenhua ChenBaihong ChenTiancai LiuYingsong WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guanfeng Lin Shaolang Chen Hui Zhao Junyu Liang Qiaoting Deng Rongliang Liang Xinxin Guo Zhenhua Chen Baihong Chen Tiancai Liu Yingsong Wu A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency
description	Abstract Replacement of the in vivo rabies vaccine potency test (NIH test) by in vitro methods had been discussed by several researcher including WHO expert working groups. In this paper, a time-resolved fluoroimmunoassay (TRFIA) for the assay of rabies virus glycoprotein in rabies vaccine was first established to estimate the rabies vaccine potency by using specific monoclonal antibody that only recognized the native, trimeric and immunogenic form of rabies virus glycoprotein. Potency of the rabies virus glycoprotein was assayed with satisfactory performance under optimal conditions, and the method demonstrated satisfactory results when applied in practical samples. The correlation coefficient of potency values obtained from the present TRFIA and ELISA was 0.912, and 0.903 for those from the present TRFIA and NIH test. These preliminary results confirmed that this TRFIA can replace ELISA with higher performance, and could be a promising replacement of the NIH test. Based upon these results, the present TRFIA seemed to be a convenient tool for evaluating rabies vaccine potency and its products at different stages accordingly.
format	article
author	Guanfeng Lin Shaolang Chen Hui Zhao Junyu Liang Qiaoting Deng Rongliang Liang Xinxin Guo Zhenhua Chen Baihong Chen Tiancai Liu Yingsong Wu
author_facet	Guanfeng Lin Shaolang Chen Hui Zhao Junyu Liang Qiaoting Deng Rongliang Liang Xinxin Guo Zhenhua Chen Baihong Chen Tiancai Liu Yingsong Wu
author_sort	Guanfeng Lin
title	A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency
title_short	A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency
title_full	A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency
title_fullStr	A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency
title_full_unstemmed	A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency
title_sort	time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fc0a269ebd254638917cd589043832a0
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A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency

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