Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation

Abstract In recent years, 3D cell culture models such as spheroid or organoid technologies have known important developments. Many studies have shown that 3D cultures exhibit better biomimetic properties compared to 2D cultures. These properties are important for in-vitro modeling systems, as well a...

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Autores principales:	Nathan Jeger-Madiot, Lousineh Arakelian, Niclas Setterblad, Patrick Bruneval, Mauricio Hoyos, Jérôme Larghero, Jean-Luc Aider
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc0ac707de294c1f9150047e791b781f
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spelling	oai:doaj.org-article:fc0ac707de294c1f9150047e791b781f2021-12-02T15:51:12ZSelf-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation10.1038/s41598-021-87459-62045-2322https://doaj.org/article/fc0ac707de294c1f9150047e791b781f2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87459-6https://doaj.org/toc/2045-2322Abstract In recent years, 3D cell culture models such as spheroid or organoid technologies have known important developments. Many studies have shown that 3D cultures exhibit better biomimetic properties compared to 2D cultures. These properties are important for in-vitro modeling systems, as well as for in-vivo cell therapies and tissue engineering approaches. A reliable use of 3D cellular models still requires standardized protocols with well-controlled and reproducible parameters. To address this challenge, a robust and scaffold-free approach is proposed, which relies on multi-trap acoustic levitation. This technology is successfully applied to Mesenchymal Stem Cells (MSCs) maintained in acoustic levitation over a 24-h period. During the culture, MSCs spontaneously self-organized from cell sheets to cell spheroids with a characteristic time of about 10 h. Each acoustofluidic chip could contain up to 30 spheroids in acoustic levitation and four chips could be ran in parallel, leading to the production of 120 spheroids per experiment. Various biological characterizations showed that the cells inside the spheroids were viable, maintained the expression of their cell surface markers and had a higher differentiation capacity compared to standard 2D culture conditions. These results open the path to long-time cell culture in acoustic levitation of cell sheets or spheroids for any type of cells.Nathan Jeger-MadiotLousineh ArakelianNiclas SetterbladPatrick BrunevalMauricio HoyosJérôme LargheroJean-Luc AiderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nathan Jeger-Madiot Lousineh Arakelian Niclas Setterblad Patrick Bruneval Mauricio Hoyos Jérôme Larghero Jean-Luc Aider Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation
description	Abstract In recent years, 3D cell culture models such as spheroid or organoid technologies have known important developments. Many studies have shown that 3D cultures exhibit better biomimetic properties compared to 2D cultures. These properties are important for in-vitro modeling systems, as well as for in-vivo cell therapies and tissue engineering approaches. A reliable use of 3D cellular models still requires standardized protocols with well-controlled and reproducible parameters. To address this challenge, a robust and scaffold-free approach is proposed, which relies on multi-trap acoustic levitation. This technology is successfully applied to Mesenchymal Stem Cells (MSCs) maintained in acoustic levitation over a 24-h period. During the culture, MSCs spontaneously self-organized from cell sheets to cell spheroids with a characteristic time of about 10 h. Each acoustofluidic chip could contain up to 30 spheroids in acoustic levitation and four chips could be ran in parallel, leading to the production of 120 spheroids per experiment. Various biological characterizations showed that the cells inside the spheroids were viable, maintained the expression of their cell surface markers and had a higher differentiation capacity compared to standard 2D culture conditions. These results open the path to long-time cell culture in acoustic levitation of cell sheets or spheroids for any type of cells.
format	article
author	Nathan Jeger-Madiot Lousineh Arakelian Niclas Setterblad Patrick Bruneval Mauricio Hoyos Jérôme Larghero Jean-Luc Aider
author_facet	Nathan Jeger-Madiot Lousineh Arakelian Niclas Setterblad Patrick Bruneval Mauricio Hoyos Jérôme Larghero Jean-Luc Aider
author_sort	Nathan Jeger-Madiot
title	Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation
title_short	Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation
title_full	Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation
title_fullStr	Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation
title_full_unstemmed	Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation
title_sort	self-organization and culture of mesenchymal stem cell spheroids in acoustic levitation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fc0ac707de294c1f9150047e791b781f
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Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation

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