Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract

Farideh Namvar,1,2 Heshu Sulaiman Rahman,3,4 Rosfarizan Mohamad,1,5 Javad Baharara,2 Mahnaz Mahdavi,6 Elaheh Amini,7 Max Stanley Chartrand,8 Swee Keong Yeap31Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, Selangor, Malaysia; 2Research Center for Animal Development Ap...

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Autores principales:	Namvar F, Rahman HS, Mohamad R, Baharara J, Mahdavi M, Amini E, Chartr, MS, Yeap SK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fc0afdbe14cd4390990064390e1af4f0
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spelling	oai:doaj.org-article:fc0afdbe14cd4390990064390e1af4f02021-12-02T02:10:26ZCytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract1178-2013https://doaj.org/article/fc0afdbe14cd4390990064390e1af4f02014-05-01T00:00:00Zhttp://www.dovepress.com/cytotoxic-effect-of-magnetic-iron-oxide-nanoparticles-synthesized-via--a16894https://doaj.org/toc/1178-2013 Farideh Namvar,1,2 Heshu Sulaiman Rahman,3,4 Rosfarizan Mohamad,1,5 Javad Baharara,2 Mahnaz Mahdavi,6 Elaheh Amini,7 Max Stanley Chartrand,8 Swee Keong Yeap31Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, Selangor, Malaysia; 2Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran; 3Institute of Bioscience, 4Department of Microbiology and Pathology, Faculty of Veterinary Medicine, 5Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia; 6Department of Chemistry, Faculty of Science, Islamic Azad University, Shiraz Branch, Shiraz, 7Kharazmi University, Tehran, Iran; 8DigiCare Behavioral Research, Casa Grande, AZ, USAAbstract: Magnetic iron oxide nanoparticles (Fe3O4 MNPs) are among the most useful metal nanoparticles for multiple applications across a broad spectrum in the biomedical field, including the diagnosis and treatment of cancer. In previous work, we synthesized and characterized Fe3O4 MNPs using a simple, rapid, safe, efficient, one-step green method involving reduction of ferric chloride solution using brown seaweed (Sargassum muticum) aqueous extract containing hydroxyl, carboxyl, and amino functional groups mainly relevant to polysaccharides, which acts as a potential stabilizer and metal reductant agent. The aim of this study was to evaluate the in vitro cytotoxic activity and cellular effects of these Fe3O4 MNPs. Their in vitro anticancer activity was demonstrated in human cell lines for leukemia (Jurkat cells), breast cancer (MCF-7 cells), cervical cancer (HeLa cells), and liver cancer (HepG2 cells). The cancer cells were treated with different concentrations of Fe3O4 MNPs, and an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was used to test for cytotoxicity, resulting in an inhibitory concentration 50 (IC50) value of 23.83±1.1 µg/mL (HepG2), 18.75±2.1 µg/mL (MCF-7), 12.5±1.7 µg/mL (HeLa), and 6.4±2.3 µg/mL (Jurkat) 72 hours after treatment. Therefore, Jurkat cells were selected for further investigation. The representative dot plots from flow cytometric analysis of apoptosis showed that the percentages of cells in early apoptosis and late apoptosis were increased. Cell cycle analysis showed a significant increase in accumulation of Fe3O4 MNP-treated cells at sub-G1 phase, confirming induction of apoptosis by Fe3O4 MNPs. The Fe3O4 MNPs also activated caspase-3 and caspase-9 in a time-response fashion. The nature of the biosynthesis and therapeutic potential of Fe3O4 MNPs could pave the way for further research on the green synthesis of therapeutic agents, particularly in nanomedicine, to assist in the treatment of cancer. Keywords: green synthesis, seaweed water extract, anticancer effect, apoptosisNamvar FRahman HSMohamad RBaharara JMahdavi MAmini EChartrMSYeap SKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2479-2488 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Namvar F Rahman HS Mohamad R Baharara J Mahdavi M Amini E Chartr MS Yeap SK Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract
description	Farideh Namvar,1,2 Heshu Sulaiman Rahman,3,4 Rosfarizan Mohamad,1,5 Javad Baharara,2 Mahnaz Mahdavi,6 Elaheh Amini,7 Max Stanley Chartrand,8 Swee Keong Yeap31Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, Selangor, Malaysia; 2Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran; 3Institute of Bioscience, 4Department of Microbiology and Pathology, Faculty of Veterinary Medicine, 5Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia; 6Department of Chemistry, Faculty of Science, Islamic Azad University, Shiraz Branch, Shiraz, 7Kharazmi University, Tehran, Iran; 8DigiCare Behavioral Research, Casa Grande, AZ, USAAbstract: Magnetic iron oxide nanoparticles (Fe3O4 MNPs) are among the most useful metal nanoparticles for multiple applications across a broad spectrum in the biomedical field, including the diagnosis and treatment of cancer. In previous work, we synthesized and characterized Fe3O4 MNPs using a simple, rapid, safe, efficient, one-step green method involving reduction of ferric chloride solution using brown seaweed (Sargassum muticum) aqueous extract containing hydroxyl, carboxyl, and amino functional groups mainly relevant to polysaccharides, which acts as a potential stabilizer and metal reductant agent. The aim of this study was to evaluate the in vitro cytotoxic activity and cellular effects of these Fe3O4 MNPs. Their in vitro anticancer activity was demonstrated in human cell lines for leukemia (Jurkat cells), breast cancer (MCF-7 cells), cervical cancer (HeLa cells), and liver cancer (HepG2 cells). The cancer cells were treated with different concentrations of Fe3O4 MNPs, and an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was used to test for cytotoxicity, resulting in an inhibitory concentration 50 (IC50) value of 23.83±1.1 µg/mL (HepG2), 18.75±2.1 µg/mL (MCF-7), 12.5±1.7 µg/mL (HeLa), and 6.4±2.3 µg/mL (Jurkat) 72 hours after treatment. Therefore, Jurkat cells were selected for further investigation. The representative dot plots from flow cytometric analysis of apoptosis showed that the percentages of cells in early apoptosis and late apoptosis were increased. Cell cycle analysis showed a significant increase in accumulation of Fe3O4 MNP-treated cells at sub-G1 phase, confirming induction of apoptosis by Fe3O4 MNPs. The Fe3O4 MNPs also activated caspase-3 and caspase-9 in a time-response fashion. The nature of the biosynthesis and therapeutic potential of Fe3O4 MNPs could pave the way for further research on the green synthesis of therapeutic agents, particularly in nanomedicine, to assist in the treatment of cancer. Keywords: green synthesis, seaweed water extract, anticancer effect, apoptosis
format	article
author	Namvar F Rahman HS Mohamad R Baharara J Mahdavi M Amini E Chartr MS Yeap SK
author_facet	Namvar F Rahman HS Mohamad R Baharara J Mahdavi M Amini E Chartr MS Yeap SK
author_sort	Namvar F
title	Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract
title_short	Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract
title_full	Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract
title_fullStr	Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract
title_full_unstemmed	Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract
title_sort	cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/fc0afdbe14cd4390990064390e1af4f0
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Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract

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