Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.

Caenorhabditis elegans has emerged as a powerful model organism for drug screening due to its cellular simplicity, genetic amenability and homology to humans combined with its small size and low cost. Currently, high-throughput drug screening assays are mostly based on image-based phenotyping with t...

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Autores principales:	Samuel Sofela, Sarah Sahloul, Yong-Ak Song
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc0b9aa926da4040928a759eec182632
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spelling	oai:doaj.org-article:fc0b9aa926da4040928a759eec1826322021-11-25T06:23:34ZBiophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.1932-620310.1371/journal.pone.0246496https://doaj.org/article/fc0b9aa926da4040928a759eec1826322021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0246496https://doaj.org/toc/1932-6203Caenorhabditis elegans has emerged as a powerful model organism for drug screening due to its cellular simplicity, genetic amenability and homology to humans combined with its small size and low cost. Currently, high-throughput drug screening assays are mostly based on image-based phenotyping with the focus on morphological-descriptive traits not exploiting key locomotory parameters of this multicellular model with muscles such as its thrashing force, a critical biophysical parameter when screening drugs for muscle-related diseases. In this study, we demonstrated the use of a micropillar-based force assay chip in combination with a fluorescence assay to evaluate the efficacy of various drugs currently used in treatment of neurodegenerative and neuromuscular diseases. Using this two-dimensional approach, we showed that the force assay was generally more sensitive in measuring efficacy of drug treatment in Duchenne Muscular Dystrophy and Parkinson's Disease mutant worms as well as partly in Amyotrophic Lateral Sclerosis model. These results underline the potential of our force assay chip in screening of potential drug candidates for the treatment of neurodegenerative and neuromuscular diseases when combined with a fluorescence assay in a two-dimensional analysis approach.Samuel SofelaSarah SahloulYong-Ak SongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0246496 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samuel Sofela Sarah Sahloul Yong-Ak Song Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.
description	Caenorhabditis elegans has emerged as a powerful model organism for drug screening due to its cellular simplicity, genetic amenability and homology to humans combined with its small size and low cost. Currently, high-throughput drug screening assays are mostly based on image-based phenotyping with the focus on morphological-descriptive traits not exploiting key locomotory parameters of this multicellular model with muscles such as its thrashing force, a critical biophysical parameter when screening drugs for muscle-related diseases. In this study, we demonstrated the use of a micropillar-based force assay chip in combination with a fluorescence assay to evaluate the efficacy of various drugs currently used in treatment of neurodegenerative and neuromuscular diseases. Using this two-dimensional approach, we showed that the force assay was generally more sensitive in measuring efficacy of drug treatment in Duchenne Muscular Dystrophy and Parkinson's Disease mutant worms as well as partly in Amyotrophic Lateral Sclerosis model. These results underline the potential of our force assay chip in screening of potential drug candidates for the treatment of neurodegenerative and neuromuscular diseases when combined with a fluorescence assay in a two-dimensional analysis approach.
format	article
author	Samuel Sofela Sarah Sahloul Yong-Ak Song
author_facet	Samuel Sofela Sarah Sahloul Yong-Ak Song
author_sort	Samuel Sofela
title	Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.
title_short	Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.
title_full	Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.
title_fullStr	Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.
title_full_unstemmed	Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.
title_sort	biophysical analysis of drug efficacy on c. elegans models for neurodegenerative and neuromuscular diseases.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/fc0b9aa926da4040928a759eec182632
work_keys_str_mv	AT samuelsofela biophysicalanalysisofdrugefficacyoncelegansmodelsforneurodegenerativeandneuromusculardiseases AT sarahsahloul biophysicalanalysisofdrugefficacyoncelegansmodelsforneurodegenerativeandneuromusculardiseases AT yongaksong biophysicalanalysisofdrugefficacyoncelegansmodelsforneurodegenerativeandneuromusculardiseases
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Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.

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