Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method

Rahmatullah SN, Jakaria, Noor RR. 2016. Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method. Biodiversitas 17: 492-497. The aim of this study was to identify the polymorphisms of the growth hormone gene (GH) of Indonesian local cattle as...

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Autores principales:	SURYA NUR RAHMATULLAH, JAKARIA JAKARIA, RONNY R NOOR
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2016
Materias:	growth hormone gene, indonesian local cattle, pcr-sscp, polymorphism Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fc0da0cc3e9c44338527a4f7be0c1ef5
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spelling	oai:doaj.org-article:fc0da0cc3e9c44338527a4f7be0c1ef52021-11-14T02:27:09ZIdentification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method1412-033X2085-472210.13057/biodiv/d170214https://doaj.org/article/fc0da0cc3e9c44338527a4f7be0c1ef52016-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/14https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Rahmatullah SN, Jakaria, Noor RR. 2016. Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method. Biodiversitas 17: 492-497. The aim of this study was to identify the polymorphisms of the growth hormone gene (GH) of Indonesian local cattle as well as two exotic cattle, as the outside group, using polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP) and using five primers DNA to identify polymorphism of GH gene. Twenty DNA samples of each Indonesian local cattle, consists of Bali, Pesisir, Madura and Katingan, and ten DNA samples of Simmental and Limousine cattle were used. The results showed that the polymorphism of the GH gene was found in three exons which are exon 1, 2 and 5 for Indonesian local cattle except for the Bali and Madura cattle that showed polymorphism was only at exon 2. Bali and Madura cattle also showed monomorphism in exon 3 and 4. On the other hand, the exotic breed showed the polymorphism in all exons, except for exon 2 in Simmental cattle which was found to be monomorphic.SURYA NUR RAHMATULLAHJAKARIA JAKARIARONNY R NOORMBI & UNS Soloarticlegrowth hormone gene, indonesian local cattle, pcr-sscp, polymorphismBiology (General)QH301-705.5ENBiodiversitas, Vol 17, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	growth hormone gene, indonesian local cattle, pcr-sscp, polymorphism Biology (General) QH301-705.5
spellingShingle	growth hormone gene, indonesian local cattle, pcr-sscp, polymorphism Biology (General) QH301-705.5 SURYA NUR RAHMATULLAH JAKARIA JAKARIA RONNY R NOOR Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method
description	Rahmatullah SN, Jakaria, Noor RR. 2016. Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method. Biodiversitas 17: 492-497. The aim of this study was to identify the polymorphisms of the growth hormone gene (GH) of Indonesian local cattle as well as two exotic cattle, as the outside group, using polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP) and using five primers DNA to identify polymorphism of GH gene. Twenty DNA samples of each Indonesian local cattle, consists of Bali, Pesisir, Madura and Katingan, and ten DNA samples of Simmental and Limousine cattle were used. The results showed that the polymorphism of the GH gene was found in three exons which are exon 1, 2 and 5 for Indonesian local cattle except for the Bali and Madura cattle that showed polymorphism was only at exon 2. Bali and Madura cattle also showed monomorphism in exon 3 and 4. On the other hand, the exotic breed showed the polymorphism in all exons, except for exon 2 in Simmental cattle which was found to be monomorphic.
format	article
author	SURYA NUR RAHMATULLAH JAKARIA JAKARIA RONNY R NOOR
author_facet	SURYA NUR RAHMATULLAH JAKARIA JAKARIA RONNY R NOOR
author_sort	SURYA NUR RAHMATULLAH
title	Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method
title_short	Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method
title_full	Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method
title_fullStr	Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method
title_full_unstemmed	Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method
title_sort	identification of growth hormone gene variation in exon region at indonesian local cattle based on pcr-sscp method
publisher	MBI & UNS Solo
publishDate	2016
url	https://doaj.org/article/fc0da0cc3e9c44338527a4f7be0c1ef5
work_keys_str_mv	AT suryanurrahmatullah identificationofgrowthhormonegenevariationinexonregionatindonesianlocalcattlebasedonpcrsscpmethod AT jakariajakaria identificationofgrowthhormonegenevariationinexonregionatindonesianlocalcattlebasedonpcrsscpmethod AT ronnyrnoor identificationofgrowthhormonegenevariationinexonregionatindonesianlocalcattlebasedonpcrsscpmethod
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Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method

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