Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors

Abstract Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in...

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Autores principales:	Koichiro Ogawa, Hidetaka Suga, Chikafumi Ozone, Mayu Sakakibara, Tomiko Yamada, Mayuko Kano, Kazuki Mitsumoto, Takatoshi Kasai, Yu Kodani, Hiroshi Nagasaki, Naoki Yamamoto, Daisuke Hagiwara, Motomitsu Goto, Ryoichi Banno, Yoshihisa Sugimura, Hiroshi Arima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc0f6bb8f18344bdaae400cfee6b074a
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spelling	oai:doaj.org-article:fc0f6bb8f18344bdaae400cfee6b074a2021-12-02T15:08:15ZVasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors10.1038/s41598-018-22053-x2045-2322https://doaj.org/article/fc0f6bb8f18344bdaae400cfee6b074a2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-22053-xhttps://doaj.org/toc/2045-2322Abstract Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embryonic developmental processes of AVP neurons. At first, the differentiation efficiency was improved. That was achieved through the optimization of the culture condition for obtaining dorsal hypothalamic progenitors. Secondly, the induced AVP neurons were identified by immunohistochemistry and these neurons secreted AVP after potassium chloride stimulation. Additionally, other hypothalamic neuropeptides were also detected, such as oxytocin, corticotropin-releasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone. This is the first report describing the generation of secretory AVP neurons derived from hESCs. This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus.Koichiro OgawaHidetaka SugaChikafumi OzoneMayu SakakibaraTomiko YamadaMayuko KanoKazuki MitsumotoTakatoshi KasaiYu KodaniHiroshi NagasakiNaoki YamamotoDaisuke HagiwaraMotomitsu GotoRyoichi BannoYoshihisa SugimuraHiroshi ArimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Koichiro Ogawa Hidetaka Suga Chikafumi Ozone Mayu Sakakibara Tomiko Yamada Mayuko Kano Kazuki Mitsumoto Takatoshi Kasai Yu Kodani Hiroshi Nagasaki Naoki Yamamoto Daisuke Hagiwara Motomitsu Goto Ryoichi Banno Yoshihisa Sugimura Hiroshi Arima Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
description	Abstract Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embryonic developmental processes of AVP neurons. At first, the differentiation efficiency was improved. That was achieved through the optimization of the culture condition for obtaining dorsal hypothalamic progenitors. Secondly, the induced AVP neurons were identified by immunohistochemistry and these neurons secreted AVP after potassium chloride stimulation. Additionally, other hypothalamic neuropeptides were also detected, such as oxytocin, corticotropin-releasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone. This is the first report describing the generation of secretory AVP neurons derived from hESCs. This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus.
format	article
author	Koichiro Ogawa Hidetaka Suga Chikafumi Ozone Mayu Sakakibara Tomiko Yamada Mayuko Kano Kazuki Mitsumoto Takatoshi Kasai Yu Kodani Hiroshi Nagasaki Naoki Yamamoto Daisuke Hagiwara Motomitsu Goto Ryoichi Banno Yoshihisa Sugimura Hiroshi Arima
author_facet	Koichiro Ogawa Hidetaka Suga Chikafumi Ozone Mayu Sakakibara Tomiko Yamada Mayuko Kano Kazuki Mitsumoto Takatoshi Kasai Yu Kodani Hiroshi Nagasaki Naoki Yamamoto Daisuke Hagiwara Motomitsu Goto Ryoichi Banno Yoshihisa Sugimura Hiroshi Arima
author_sort	Koichiro Ogawa
title	Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_short	Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_full	Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_fullStr	Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_full_unstemmed	Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
title_sort	vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fc0f6bb8f18344bdaae400cfee6b074a
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Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors

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