Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea

Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the fae...

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Autores principales:	Chengyuan Ji, Yao Zhang, Ruini Sun, Jiale Ma, Zihao Pan, Huochun Yao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bovine enterovirus isolation identification genetic analysis phylogenetic analysis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fc146485765a498788b01b11bd6af812
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spelling	oai:doaj.org-article:fc146485765a498788b01b11bd6af8122021-11-25T19:13:25ZIsolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea10.3390/v131122171999-4915https://doaj.org/article/fc146485765a498788b01b11bd6af8122021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2217https://doaj.org/toc/1999-4915Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the faeces of cows with significant diarrhoeal symptoms in China and observed the viral particles within 20–30 nm through transmission electron microscopy. Then, we designated this strain as HB19-1 in this study. The multistep growth curves showed that the virus propagated well in the MDBK cells. Molecular genetic analysis of VP1 indicated that HB19-1 belonged to the BEV-F1 group. Although the challenged ICR mice did not exhibit typical disease symptoms in animal infection assay, we observed significant pathological damage in the lungs, intestines, and muscle tissues. In summary, we isolated a BEV strain HB19-1 causing severe diarrhoea in cattle and proposed reinforcing the epidemiological surveillance of this virus.Chengyuan JiYao ZhangRuini SunJiale MaZihao PanHuochun YaoMDPI AGarticlebovine enterovirusisolationidentificationgenetic analysisphylogenetic analysisMicrobiologyQR1-502ENViruses, Vol 13, Iss 2217, p 2217 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine enterovirus isolation identification genetic analysis phylogenetic analysis Microbiology QR1-502
spellingShingle	bovine enterovirus isolation identification genetic analysis phylogenetic analysis Microbiology QR1-502 Chengyuan Ji Yao Zhang Ruini Sun Jiale Ma Zihao Pan Huochun Yao Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
description	Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the faeces of cows with significant diarrhoeal symptoms in China and observed the viral particles within 20–30 nm through transmission electron microscopy. Then, we designated this strain as HB19-1 in this study. The multistep growth curves showed that the virus propagated well in the MDBK cells. Molecular genetic analysis of VP1 indicated that HB19-1 belonged to the BEV-F1 group. Although the challenged ICR mice did not exhibit typical disease symptoms in animal infection assay, we observed significant pathological damage in the lungs, intestines, and muscle tissues. In summary, we isolated a BEV strain HB19-1 causing severe diarrhoea in cattle and proposed reinforcing the epidemiological surveillance of this virus.
format	article
author	Chengyuan Ji Yao Zhang Ruini Sun Jiale Ma Zihao Pan Huochun Yao
author_facet	Chengyuan Ji Yao Zhang Ruini Sun Jiale Ma Zihao Pan Huochun Yao
author_sort	Chengyuan Ji
title	Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_short	Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_full	Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_fullStr	Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_full_unstemmed	Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_sort	isolation and identification of type f bovine enterovirus from clinical cattle with diarrhoea
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fc146485765a498788b01b11bd6af812
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Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea

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