The effect of surface nanometre-scale morphology on protein adsorption.

<h4>Background</h4>Protein adsorption is the first of a complex series of events that regulates many phenomena at the nano-bio interface, e.g. cell adhesion and differentiation, in vivo inflammatory responses and protein crystallization. A quantitative understanding of how nanoscale morp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pasquale Emanuele Scopelliti, Antonio Borgonovo, Marco Indrieri, Luca Giorgetti, Gero Bongiorno, Roberta Carbone, Alessandro Podestà, Paolo Milani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc158b717bd2402dab016192ad52986e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fc158b717bd2402dab016192ad52986e
record_format	dspace
spelling	oai:doaj.org-article:fc158b717bd2402dab016192ad52986e2021-11-18T06:36:35ZThe effect of surface nanometre-scale morphology on protein adsorption.1932-620310.1371/journal.pone.0011862https://doaj.org/article/fc158b717bd2402dab016192ad52986e2010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20686681/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Protein adsorption is the first of a complex series of events that regulates many phenomena at the nano-bio interface, e.g. cell adhesion and differentiation, in vivo inflammatory responses and protein crystallization. A quantitative understanding of how nanoscale morphology influences protein adsorption is strategic for providing insight into all of these processes, however this understanding has been lacking until now.<h4>Methodology/principal findings</h4>Here we introduce novel methods for quantitative high-throughput characterization of protein-surface interaction and we apply them in an integrated experimental strategy, to study the adsorption of a panel of proteins on nanostructured surfaces. We show that the increase of nanoscale roughness (from 15 nm to 30 nm) induces a decrease of protein binding affinity (<or=90%) and a relevant increase in adsorbed proteins (<or=500%) beyond the corresponding increase of specific area. We demonstrate that these effects are caused by protein nucleation on the surface, which is promoted by surface nanoscale pores.<h4>Conclusions/significance</h4>These results show that the adsorption of proteins depends significantly on surface nanostructure and that the relevant morphological parameter regulating the protein adsorption process is the nanometric pore shape. These new findings improve our understanding of the role of nanostructures as a biomaterial design parameter and they have important implications for the general understanding of cell behavior on nanostructured surfaces.Pasquale Emanuele ScopellitiAntonio BorgonovoMarco IndrieriLuca GiorgettiGero BongiornoRoberta CarboneAlessandro PodestàPaolo MilaniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11862 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pasquale Emanuele Scopelliti Antonio Borgonovo Marco Indrieri Luca Giorgetti Gero Bongiorno Roberta Carbone Alessandro Podestà Paolo Milani The effect of surface nanometre-scale morphology on protein adsorption.
description	<h4>Background</h4>Protein adsorption is the first of a complex series of events that regulates many phenomena at the nano-bio interface, e.g. cell adhesion and differentiation, in vivo inflammatory responses and protein crystallization. A quantitative understanding of how nanoscale morphology influences protein adsorption is strategic for providing insight into all of these processes, however this understanding has been lacking until now.<h4>Methodology/principal findings</h4>Here we introduce novel methods for quantitative high-throughput characterization of protein-surface interaction and we apply them in an integrated experimental strategy, to study the adsorption of a panel of proteins on nanostructured surfaces. We show that the increase of nanoscale roughness (from 15 nm to 30 nm) induces a decrease of protein binding affinity (<or=90%) and a relevant increase in adsorbed proteins (<or=500%) beyond the corresponding increase of specific area. We demonstrate that these effects are caused by protein nucleation on the surface, which is promoted by surface nanoscale pores.<h4>Conclusions/significance</h4>These results show that the adsorption of proteins depends significantly on surface nanostructure and that the relevant morphological parameter regulating the protein adsorption process is the nanometric pore shape. These new findings improve our understanding of the role of nanostructures as a biomaterial design parameter and they have important implications for the general understanding of cell behavior on nanostructured surfaces.
format	article
author	Pasquale Emanuele Scopelliti Antonio Borgonovo Marco Indrieri Luca Giorgetti Gero Bongiorno Roberta Carbone Alessandro Podestà Paolo Milani
author_facet	Pasquale Emanuele Scopelliti Antonio Borgonovo Marco Indrieri Luca Giorgetti Gero Bongiorno Roberta Carbone Alessandro Podestà Paolo Milani
author_sort	Pasquale Emanuele Scopelliti
title	The effect of surface nanometre-scale morphology on protein adsorption.
title_short	The effect of surface nanometre-scale morphology on protein adsorption.
title_full	The effect of surface nanometre-scale morphology on protein adsorption.
title_fullStr	The effect of surface nanometre-scale morphology on protein adsorption.
title_full_unstemmed	The effect of surface nanometre-scale morphology on protein adsorption.
title_sort	effect of surface nanometre-scale morphology on protein adsorption.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/fc158b717bd2402dab016192ad52986e
work_keys_str_mv	AT pasqualeemanuelescopelliti theeffectofsurfacenanometrescalemorphologyonproteinadsorption AT antonioborgonovo theeffectofsurfacenanometrescalemorphologyonproteinadsorption AT marcoindrieri theeffectofsurfacenanometrescalemorphologyonproteinadsorption AT lucagiorgetti theeffectofsurfacenanometrescalemorphologyonproteinadsorption AT gerobongiorno theeffectofsurfacenanometrescalemorphologyonproteinadsorption AT robertacarbone theeffectofsurfacenanometrescalemorphologyonproteinadsorption AT alessandropodesta theeffectofsurfacenanometrescalemorphologyonproteinadsorption AT paolomilani theeffectofsurfacenanometrescalemorphologyonproteinadsorption AT pasqualeemanuelescopelliti effectofsurfacenanometrescalemorphologyonproteinadsorption AT antonioborgonovo effectofsurfacenanometrescalemorphologyonproteinadsorption AT marcoindrieri effectofsurfacenanometrescalemorphologyonproteinadsorption AT lucagiorgetti effectofsurfacenanometrescalemorphologyonproteinadsorption AT gerobongiorno effectofsurfacenanometrescalemorphologyonproteinadsorption AT robertacarbone effectofsurfacenanometrescalemorphologyonproteinadsorption AT alessandropodesta effectofsurfacenanometrescalemorphologyonproteinadsorption AT paolomilani effectofsurfacenanometrescalemorphologyonproteinadsorption
_version_	1718424454968639488

The effect of surface nanometre-scale morphology on protein adsorption.

Ejemplares similares