Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin

Mothanna Sadiq Al-Qubaisi,1 Abdullah Rasedee,1,2 Moayad Husein Flaifel,5 Sahrim HJ Ahmad,5 Samer Hussein-Al-Ali,1 Mohd Zobir Hussein,3 Eltayeb EM Eid,6 Zulkarnain Zainal,3 Mohd Saeed,1 Muna Ilowefah,4 Sharida Fakurazi,1 Norhaszalina Mohd Isa,1 Mohamed Ezzat El Zowalaty1,7 1Institute of Bioscience, 2...

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Autores principales:	Al-Qubaisi MS, Rasedee A, Flaifel MH, Ahmad SHJ, Hussein-Al-Ali S, Hussein MZ, Eid EEM, Zainal Z, Saeed M, Ilowefah M, Fakurazi S, Mohd Isa N, El Zowalaty ME
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fc19e489779a4c43b134150760c6dc04
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spelling	oai:doaj.org-article:fc19e489779a4c43b134150760c6dc042021-12-02T11:10:52ZCytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin1176-91141178-2013https://doaj.org/article/fc19e489779a4c43b134150760c6dc042013-07-01T00:00:00Zhttp://www.dovepress.com/cytotoxicity-of-nickel-zinc-ferrite-nanoparticles-on-cancer-cells-of-e-a13665https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Mothanna Sadiq Al-Qubaisi,1 Abdullah Rasedee,1,2 Moayad Husein Flaifel,5 Sahrim HJ Ahmad,5 Samer Hussein-Al-Ali,1 Mohd Zobir Hussein,3 Eltayeb EM Eid,6 Zulkarnain Zainal,3 Mohd Saeed,1 Muna Ilowefah,4 Sharida Fakurazi,1 Norhaszalina Mohd Isa,1 Mohamed Ezzat El Zowalaty1,7 1Institute of Bioscience, 2Faculty of Veterinary Medicine, 3Department of Chemistry, Faculty of Science, 4Faculty of Food Science and Technology, Universiti Putra Malaysia, Selangor, Malaysia; 5School of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, Malaysia; 6College of Pharmacy, Qassim University, Buraidah, Saudi Arabia; 7Faculty of Pharmacy, Zagazig University, Ash Sharqiyah, Egypt Abstract: In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nanoparticles (15.6–1,000 µg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were stimulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells. Keywords: NiZn ferrite nanoparticles, cancer cells lines, anticancer, apoptosisAl-Qubaisi MSRasedee AFlaifel MHAhmad SHJHussein-Al-Ali SHussein MZEid EEMZainal ZSaeed MIlowefah MFakurazi SMohd Isa NEl Zowalaty MEDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2497-2508 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Al-Qubaisi MS Rasedee A Flaifel MH Ahmad SHJ Hussein-Al-Ali S Hussein MZ Eid EEM Zainal Z Saeed M Ilowefah M Fakurazi S Mohd Isa N El Zowalaty ME Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin
description	Mothanna Sadiq Al-Qubaisi,1 Abdullah Rasedee,1,2 Moayad Husein Flaifel,5 Sahrim HJ Ahmad,5 Samer Hussein-Al-Ali,1 Mohd Zobir Hussein,3 Eltayeb EM Eid,6 Zulkarnain Zainal,3 Mohd Saeed,1 Muna Ilowefah,4 Sharida Fakurazi,1 Norhaszalina Mohd Isa,1 Mohamed Ezzat El Zowalaty1,7 1Institute of Bioscience, 2Faculty of Veterinary Medicine, 3Department of Chemistry, Faculty of Science, 4Faculty of Food Science and Technology, Universiti Putra Malaysia, Selangor, Malaysia; 5School of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, Malaysia; 6College of Pharmacy, Qassim University, Buraidah, Saudi Arabia; 7Faculty of Pharmacy, Zagazig University, Ash Sharqiyah, Egypt Abstract: In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nanoparticles (15.6–1,000 µg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were stimulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells. Keywords: NiZn ferrite nanoparticles, cancer cells lines, anticancer, apoptosis
format	article
author	Al-Qubaisi MS Rasedee A Flaifel MH Ahmad SHJ Hussein-Al-Ali S Hussein MZ Eid EEM Zainal Z Saeed M Ilowefah M Fakurazi S Mohd Isa N El Zowalaty ME
author_facet	Al-Qubaisi MS Rasedee A Flaifel MH Ahmad SHJ Hussein-Al-Ali S Hussein MZ Eid EEM Zainal Z Saeed M Ilowefah M Fakurazi S Mohd Isa N El Zowalaty ME
author_sort	Al-Qubaisi MS
title	Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin
title_short	Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin
title_full	Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin
title_fullStr	Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin
title_full_unstemmed	Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin
title_sort	cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/fc19e489779a4c43b134150760c6dc04
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Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin

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