Gamma-Irradiated Fowl Cholera Mucosal Vaccine: Potential Vaccine Candidate for Safe and Effective Immunization of Chicken Against Fowl Cholera

Fowl cholera (FC) caused by Pasteurella multocida is among the serious infectious diseases of poultry. Currently, formalin inactivated FC (FI-FC) vaccine is widely used in Ethiopia. However, reports of the disease complaint remain higher despite the use of the vaccine. The aim of this study was to d...

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Autores principales:	Bereket Dessalegn, Molalegne Bitew, Destaw Asfaw, Esraa Khojaly, Saddam Mohammed Ibrahim, Takele Abayneh, Esayas Gelaye, Hermann Unger, Viskam Wijewardana
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	chicken fowl cholera gamma radiation mucosal vaccine P. multocida Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/fc1ae133d7024fa29c6535068426d202
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spelling	oai:doaj.org-article:fc1ae133d7024fa29c6535068426d2022021-12-01T19:37:31ZGamma-Irradiated Fowl Cholera Mucosal Vaccine: Potential Vaccine Candidate for Safe and Effective Immunization of Chicken Against Fowl Cholera1664-322410.3389/fimmu.2021.768820https://doaj.org/article/fc1ae133d7024fa29c6535068426d2022021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.768820/fullhttps://doaj.org/toc/1664-3224Fowl cholera (FC) caused by Pasteurella multocida is among the serious infectious diseases of poultry. Currently, formalin inactivated FC (FI-FC) vaccine is widely used in Ethiopia. However, reports of the disease complaint remain higher despite the use of the vaccine. The aim of this study was to develop and evaluate gamma-irradiated mucosal FC vaccines that can be used nationally. In a vaccination-challenge experiment, the performance of gamma-irradiated P. multocida (at 1 kGy) formulated with Montanide gel/01 PR adjuvant was evaluated at different dose rates (0.5 and 0.3 ml) and routes (intranasal, intraocular, and oral), in comparison with FI-FC vaccine in chicken. Chickens received three doses of the candidate vaccine at 3-week intervals. Sera, and trachea and crop lavage were collected to assess the antibody levels using indirect and sandwich ELISAs, respectively. Challenge exposure was conducted by inoculation at 3.5×109 CFU/ml of P. multocida biotype A intranasally 2 weeks after the last immunization. Repeated measures ANOVA test and Kaplan Meier curve analysis were used to examine for statistical significance of antibody titers and survival analysis, respectively. Sera IgG and secretory IgA titers were significantly raised after second immunization (p=0.0001). Chicken survival analysis showed that intranasal and intraocular administration of the candidate vaccine at the dose of 0.3 ml resulted in 100% protection as compared to intramuscular injection of FI-FC vaccine, which conferred 85% protection (p=0.002). In conclusion, the results of this study showed that gamma-irradiated FC mucosal vaccine is safe and protective, indicating its potential use for immunization of chicken against FC.Bereket DessalegnMolalegne BitewDestaw AsfawEsraa KhojalySaddam Mohammed IbrahimTakele AbaynehEsayas GelayeHermann UngerViskam WijewardanaFrontiers Media S.A.articlechickenfowl choleragamma radiationmucosal vaccineP. multocidaImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chicken fowl cholera gamma radiation mucosal vaccine P. multocida Immunologic diseases. Allergy RC581-607
spellingShingle	chicken fowl cholera gamma radiation mucosal vaccine P. multocida Immunologic diseases. Allergy RC581-607 Bereket Dessalegn Molalegne Bitew Destaw Asfaw Esraa Khojaly Saddam Mohammed Ibrahim Takele Abayneh Esayas Gelaye Hermann Unger Viskam Wijewardana Gamma-Irradiated Fowl Cholera Mucosal Vaccine: Potential Vaccine Candidate for Safe and Effective Immunization of Chicken Against Fowl Cholera
description	Fowl cholera (FC) caused by Pasteurella multocida is among the serious infectious diseases of poultry. Currently, formalin inactivated FC (FI-FC) vaccine is widely used in Ethiopia. However, reports of the disease complaint remain higher despite the use of the vaccine. The aim of this study was to develop and evaluate gamma-irradiated mucosal FC vaccines that can be used nationally. In a vaccination-challenge experiment, the performance of gamma-irradiated P. multocida (at 1 kGy) formulated with Montanide gel/01 PR adjuvant was evaluated at different dose rates (0.5 and 0.3 ml) and routes (intranasal, intraocular, and oral), in comparison with FI-FC vaccine in chicken. Chickens received three doses of the candidate vaccine at 3-week intervals. Sera, and trachea and crop lavage were collected to assess the antibody levels using indirect and sandwich ELISAs, respectively. Challenge exposure was conducted by inoculation at 3.5×109 CFU/ml of P. multocida biotype A intranasally 2 weeks after the last immunization. Repeated measures ANOVA test and Kaplan Meier curve analysis were used to examine for statistical significance of antibody titers and survival analysis, respectively. Sera IgG and secretory IgA titers were significantly raised after second immunization (p=0.0001). Chicken survival analysis showed that intranasal and intraocular administration of the candidate vaccine at the dose of 0.3 ml resulted in 100% protection as compared to intramuscular injection of FI-FC vaccine, which conferred 85% protection (p=0.002). In conclusion, the results of this study showed that gamma-irradiated FC mucosal vaccine is safe and protective, indicating its potential use for immunization of chicken against FC.
format	article
author	Bereket Dessalegn Molalegne Bitew Destaw Asfaw Esraa Khojaly Saddam Mohammed Ibrahim Takele Abayneh Esayas Gelaye Hermann Unger Viskam Wijewardana
author_facet	Bereket Dessalegn Molalegne Bitew Destaw Asfaw Esraa Khojaly Saddam Mohammed Ibrahim Takele Abayneh Esayas Gelaye Hermann Unger Viskam Wijewardana
author_sort	Bereket Dessalegn
title	Gamma-Irradiated Fowl Cholera Mucosal Vaccine: Potential Vaccine Candidate for Safe and Effective Immunization of Chicken Against Fowl Cholera
title_short	Gamma-Irradiated Fowl Cholera Mucosal Vaccine: Potential Vaccine Candidate for Safe and Effective Immunization of Chicken Against Fowl Cholera
title_full	Gamma-Irradiated Fowl Cholera Mucosal Vaccine: Potential Vaccine Candidate for Safe and Effective Immunization of Chicken Against Fowl Cholera
title_fullStr	Gamma-Irradiated Fowl Cholera Mucosal Vaccine: Potential Vaccine Candidate for Safe and Effective Immunization of Chicken Against Fowl Cholera
title_full_unstemmed	Gamma-Irradiated Fowl Cholera Mucosal Vaccine: Potential Vaccine Candidate for Safe and Effective Immunization of Chicken Against Fowl Cholera
title_sort	gamma-irradiated fowl cholera mucosal vaccine: potential vaccine candidate for safe and effective immunization of chicken against fowl cholera
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fc1ae133d7024fa29c6535068426d202
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Gamma-Irradiated Fowl Cholera Mucosal Vaccine: Potential Vaccine Candidate for Safe and Effective Immunization of Chicken Against Fowl Cholera

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