In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence

Trehalose-6-phosphate phosphatase (TPP) genes take part in trehalose metabolism and also in stress tolerance, which has been well documented in many species but poorly understood in wheat. The present research has identified a family of 31 TPP genes in ...

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Autores principales:	Md Ashraful Islam, Md Mustafizur Rahman, Md Mizanor Rahman, Xiujuan Jin, Lili Sun, Kai Zhao, Shuguang Wang, Ashim Sikdar, Hafeez Noor, Jong-Seong Jeon, Wenjun Zhang, Daizhen Sun
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	in silico <i>Cis</i>-regulatory elements gene transcription trehalose-6-phosphate phosphatase wheat Genetics QH426-470
Acceso en línea:	https://doaj.org/article/fc28090348fe4e0183651b8505c9163e
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spelling	oai:doaj.org-article:fc28090348fe4e0183651b8505c9163e2021-11-25T17:40:14ZIn Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence10.3390/genes121116522073-4425https://doaj.org/article/fc28090348fe4e0183651b8505c9163e2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1652https://doaj.org/toc/2073-4425Trehalose-6-phosphate phosphatase (<i>TPP</i>) genes take part in trehalose metabolism and also in stress tolerance, which has been well documented in many species but poorly understood in wheat. The present research has identified a family of 31 <i>TPP</i> genes in <i>Triticum aestivum</i> L. through homology searches and classified them into five clades by phylogenetic tree analysis, providing evidence of an evolutionary status with <i>Hordeum vulgare, Brachypodium distachyon</i> and <i>Oryza sativa.</i> The exon-intron distribution revealed a discrete evolutionary history and projected possible gene duplication occurrences. Furthermore, different computational approaches were used to analyze the physical and chemical properties, conserved domains and motifs, subcellular and chromosomal localization, and three-dimensional (3-D) protein structures. <i>Cis</i>-regulatory elements (CREs) analysis predicted that <i>TaTPP</i> promoters consist of CREs related to plant growth and development, hormones, and stress. Transcriptional analysis revealed that the transcription levels of <i>TaTPPs</i> were variable in different developmental stages and organs. In addition, qRT-PCR analysis showed that different <i>TaTPPs</i> were induced under salt and drought stresses and during leaf senescence. Therefore, the findings of the present study give fundamental genomic information and possible biological functions of the <i>TaTPP</i> gene family in wheat and will provide the path for a better understanding of <i>TaTPPs</i> involvement in wheat developmental processes, stress tolerance, and leaf senescence.Md Ashraful IslamMd Mustafizur RahmanMd Mizanor RahmanXiujuan JinLili SunKai ZhaoShuguang WangAshim SikdarHafeez NoorJong-Seong JeonWenjun ZhangDaizhen SunMDPI AGarticlein silico<i>Cis</i>-regulatory elementsgene transcriptiontrehalose-6-phosphate phosphatasewheatGeneticsQH426-470ENGenes, Vol 12, Iss 1652, p 1652 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	in silico <i>Cis</i>-regulatory elements gene transcription trehalose-6-phosphate phosphatase wheat Genetics QH426-470
spellingShingle	in silico <i>Cis</i>-regulatory elements gene transcription trehalose-6-phosphate phosphatase wheat Genetics QH426-470 Md Ashraful Islam Md Mustafizur Rahman Md Mizanor Rahman Xiujuan Jin Lili Sun Kai Zhao Shuguang Wang Ashim Sikdar Hafeez Noor Jong-Seong Jeon Wenjun Zhang Daizhen Sun In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence
description	Trehalose-6-phosphate phosphatase (<i>TPP</i>) genes take part in trehalose metabolism and also in stress tolerance, which has been well documented in many species but poorly understood in wheat. The present research has identified a family of 31 <i>TPP</i> genes in <i>Triticum aestivum</i> L. through homology searches and classified them into five clades by phylogenetic tree analysis, providing evidence of an evolutionary status with <i>Hordeum vulgare, Brachypodium distachyon</i> and <i>Oryza sativa.</i> The exon-intron distribution revealed a discrete evolutionary history and projected possible gene duplication occurrences. Furthermore, different computational approaches were used to analyze the physical and chemical properties, conserved domains and motifs, subcellular and chromosomal localization, and three-dimensional (3-D) protein structures. <i>Cis</i>-regulatory elements (CREs) analysis predicted that <i>TaTPP</i> promoters consist of CREs related to plant growth and development, hormones, and stress. Transcriptional analysis revealed that the transcription levels of <i>TaTPPs</i> were variable in different developmental stages and organs. In addition, qRT-PCR analysis showed that different <i>TaTPPs</i> were induced under salt and drought stresses and during leaf senescence. Therefore, the findings of the present study give fundamental genomic information and possible biological functions of the <i>TaTPP</i> gene family in wheat and will provide the path for a better understanding of <i>TaTPPs</i> involvement in wheat developmental processes, stress tolerance, and leaf senescence.
format	article
author	Md Ashraful Islam Md Mustafizur Rahman Md Mizanor Rahman Xiujuan Jin Lili Sun Kai Zhao Shuguang Wang Ashim Sikdar Hafeez Noor Jong-Seong Jeon Wenjun Zhang Daizhen Sun
author_facet	Md Ashraful Islam Md Mustafizur Rahman Md Mizanor Rahman Xiujuan Jin Lili Sun Kai Zhao Shuguang Wang Ashim Sikdar Hafeez Noor Jong-Seong Jeon Wenjun Zhang Daizhen Sun
author_sort	Md Ashraful Islam
title	In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence
title_short	In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence
title_full	In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence
title_fullStr	In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence
title_full_unstemmed	In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence
title_sort	in silico and transcription analysis of trehalose-6-phosphate phosphatase gene family of wheat: trehalose synthesis genes contribute to salinity, drought stress and leaf senescence
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fc28090348fe4e0183651b8505c9163e
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In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence

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