Comparative transcriptome analysis of unripe and ripe banana (cv. Nendran) unraveling genes involved in ripening and other related processes.

Banana is one of the most important fruit crops consumed globally owing to its high nutritional value. Previously, we demonstrated that the ripe pulp of the banana cultivar (cv.) Nendran (AAB) contained a high amount of pro-vitamin A carotenoids. However, the molecular factors involved in the ripeni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Karambir Kaur, Praveen Awasthi, Siddharth Tiwari
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc286573087a4bee97a9201d8823d46a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fc286573087a4bee97a9201d8823d46a
record_format	dspace
spelling	oai:doaj.org-article:fc286573087a4bee97a9201d8823d46a2021-12-02T20:06:25ZComparative transcriptome analysis of unripe and ripe banana (cv. Nendran) unraveling genes involved in ripening and other related processes.1932-620310.1371/journal.pone.0254709https://doaj.org/article/fc286573087a4bee97a9201d8823d46a2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254709https://doaj.org/toc/1932-6203Banana is one of the most important fruit crops consumed globally owing to its high nutritional value. Previously, we demonstrated that the ripe pulp of the banana cultivar (cv.) Nendran (AAB) contained a high amount of pro-vitamin A carotenoids. However, the molecular factors involved in the ripening process in Nendran fruit are unexplored. Hence, we commenced a transcriptome study by using the Illumina HiSeq 2500 at two stages i.e. unripe and ripe fruit-pulp of Nendran. Overall, 3474 up and 4727 down-regulated genes were obtained. A large number of identified transcripts were related to genes involved in ripening, cell wall degradation and aroma formation. Gene ontology analysis highlighted differentially expressed genes that play a key role in various pathways. These pathways were mainly linked to cellular, molecular and biological processes. The present transcriptome study also reveals a crucial role of up-regulated carotenoid biosynthesis pathway genes namely, lycopene beta cyclase and geranylgeranyl pyrophosphate synthase at the ripening stage. Genes related to the ripening and other processes like aroma and flavor were highly expressed in the ripe pulp. Expression of numerous transcription factor family genes was also identified. This study lays a path towards understanding the ripening, carotenoid accumulation and other related processes in banana.Karambir KaurPraveen AwasthiSiddharth TiwariPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254709 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karambir Kaur Praveen Awasthi Siddharth Tiwari Comparative transcriptome analysis of unripe and ripe banana (cv. Nendran) unraveling genes involved in ripening and other related processes.
description	Banana is one of the most important fruit crops consumed globally owing to its high nutritional value. Previously, we demonstrated that the ripe pulp of the banana cultivar (cv.) Nendran (AAB) contained a high amount of pro-vitamin A carotenoids. However, the molecular factors involved in the ripening process in Nendran fruit are unexplored. Hence, we commenced a transcriptome study by using the Illumina HiSeq 2500 at two stages i.e. unripe and ripe fruit-pulp of Nendran. Overall, 3474 up and 4727 down-regulated genes were obtained. A large number of identified transcripts were related to genes involved in ripening, cell wall degradation and aroma formation. Gene ontology analysis highlighted differentially expressed genes that play a key role in various pathways. These pathways were mainly linked to cellular, molecular and biological processes. The present transcriptome study also reveals a crucial role of up-regulated carotenoid biosynthesis pathway genes namely, lycopene beta cyclase and geranylgeranyl pyrophosphate synthase at the ripening stage. Genes related to the ripening and other processes like aroma and flavor were highly expressed in the ripe pulp. Expression of numerous transcription factor family genes was also identified. This study lays a path towards understanding the ripening, carotenoid accumulation and other related processes in banana.
format	article
author	Karambir Kaur Praveen Awasthi Siddharth Tiwari
author_facet	Karambir Kaur Praveen Awasthi Siddharth Tiwari
author_sort	Karambir Kaur
title	Comparative transcriptome analysis of unripe and ripe banana (cv. Nendran) unraveling genes involved in ripening and other related processes.
title_short	Comparative transcriptome analysis of unripe and ripe banana (cv. Nendran) unraveling genes involved in ripening and other related processes.
title_full	Comparative transcriptome analysis of unripe and ripe banana (cv. Nendran) unraveling genes involved in ripening and other related processes.
title_fullStr	Comparative transcriptome analysis of unripe and ripe banana (cv. Nendran) unraveling genes involved in ripening and other related processes.
title_full_unstemmed	Comparative transcriptome analysis of unripe and ripe banana (cv. Nendran) unraveling genes involved in ripening and other related processes.
title_sort	comparative transcriptome analysis of unripe and ripe banana (cv. nendran) unraveling genes involved in ripening and other related processes.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/fc286573087a4bee97a9201d8823d46a
work_keys_str_mv	AT karambirkaur comparativetranscriptomeanalysisofunripeandripebananacvnendranunravelinggenesinvolvedinripeningandotherrelatedprocesses AT praveenawasthi comparativetranscriptomeanalysisofunripeandripebananacvnendranunravelinggenesinvolvedinripeningandotherrelatedprocesses AT siddharthtiwari comparativetranscriptomeanalysisofunripeandripebananacvnendranunravelinggenesinvolvedinripeningandotherrelatedprocesses
_version_	1718375399940947968

Comparative transcriptome analysis of unripe and ripe banana (cv. Nendran) unraveling genes involved in ripening and other related processes.

Ejemplares similares