Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression

Intra-articular injection of mesenchymal stem cells (MSCs) is a promising strategy for osteoarthritis (OA) treatment. However, more and more studies reveal that the injected MSCs have poor adhesion, migration, and survival in the joint cavity. A recent study shows that tropoelastin (TE) regulates ad...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Junjun Yang, Xin Wang, Yahan Fan, Xiongbo Song, Jiangyi Wu, Zhenlan Fu, Tao Li, Yang Huang, ZheXiong Tang, Shuo Meng, Na Liu, Jiajia Chen, Pingju Liu, Liu Yang, Xiaoyuan Gong, Cheng Chen
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Tropoelastin Osteoarthritis Infrapatellar fat pad MSCs Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fc292ee3ec3a4903a8ed6c324d31e6e7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fc292ee3ec3a4903a8ed6c324d31e6e7
record_format	dspace
spelling	oai:doaj.org-article:fc292ee3ec3a4903a8ed6c324d31e6e72021-11-28T04:35:36ZTropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression2452-199X10.1016/j.bioactmat.2021.09.011https://doaj.org/article/fc292ee3ec3a4903a8ed6c324d31e6e72022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X2100428Xhttps://doaj.org/toc/2452-199XIntra-articular injection of mesenchymal stem cells (MSCs) is a promising strategy for osteoarthritis (OA) treatment. However, more and more studies reveal that the injected MSCs have poor adhesion, migration, and survival in the joint cavity. A recent study shows that tropoelastin (TE) regulates adhesion, proliferation and phenotypic maintenance of MSCs as a soluble additive, indicating that TE could promote MSCs-homing in regenerative medicine. In this study, we used TE as injection medium, and compared it with classic media in MSCs intra-articular injection such as normal saline (NS), hyaluronic acid (HA), and platelet-rich plasma (PRP). We found that TE could effectively improve adhesion, migration, chondrogenic differentiation of infrapatellar fat pad MSCs (IPFP-MSCs) and enhance matrix synthesis of osteoarthritic chondrocytes (OACs) in indirect-coculture system. Moreover, TE could significantly enhance IPFP-MSCs adhesion via activation of integrin β1, ERK1/2 and vinculin (VCL) in vitro. In addition, intra-articular injection of TE-IPFP MSCs suspension resulted in a short-term increase in survival rate of IPFP-MSCs and better histology scores of rat joint tissues. Inhibition of integrin β1 or ERK1/2 attenuated the protective effect of TE-IPFP MSCs suspension in vivo. In conclusion, TE promotes performance of IPFP-MSCs and protects knee cartilage from damage in OA through enhancement of cell adhesion and activation of integrin β1/ERK/VCL pathway. Our ﬁndings may provide new insights in MSCs intra-articular injection for OA treatment.Junjun YangXin WangYahan FanXiongbo SongJiangyi WuZhenlan FuTao LiYang HuangZheXiong TangShuo MengNa LiuJiajia ChenPingju LiuLiu YangXiaoyuan GongCheng ChenKeAi Communications Co., Ltd.articleTropoelastinOsteoarthritisInfrapatellar fat pad MSCsMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 443-459 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tropoelastin Osteoarthritis Infrapatellar fat pad MSCs Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Tropoelastin Osteoarthritis Infrapatellar fat pad MSCs Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Junjun Yang Xin Wang Yahan Fan Xiongbo Song Jiangyi Wu Zhenlan Fu Tao Li Yang Huang ZheXiong Tang Shuo Meng Na Liu Jiajia Chen Pingju Liu Liu Yang Xiaoyuan Gong Cheng Chen Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression
description	Intra-articular injection of mesenchymal stem cells (MSCs) is a promising strategy for osteoarthritis (OA) treatment. However, more and more studies reveal that the injected MSCs have poor adhesion, migration, and survival in the joint cavity. A recent study shows that tropoelastin (TE) regulates adhesion, proliferation and phenotypic maintenance of MSCs as a soluble additive, indicating that TE could promote MSCs-homing in regenerative medicine. In this study, we used TE as injection medium, and compared it with classic media in MSCs intra-articular injection such as normal saline (NS), hyaluronic acid (HA), and platelet-rich plasma (PRP). We found that TE could effectively improve adhesion, migration, chondrogenic differentiation of infrapatellar fat pad MSCs (IPFP-MSCs) and enhance matrix synthesis of osteoarthritic chondrocytes (OACs) in indirect-coculture system. Moreover, TE could significantly enhance IPFP-MSCs adhesion via activation of integrin β1, ERK1/2 and vinculin (VCL) in vitro. In addition, intra-articular injection of TE-IPFP MSCs suspension resulted in a short-term increase in survival rate of IPFP-MSCs and better histology scores of rat joint tissues. Inhibition of integrin β1 or ERK1/2 attenuated the protective effect of TE-IPFP MSCs suspension in vivo. In conclusion, TE promotes performance of IPFP-MSCs and protects knee cartilage from damage in OA through enhancement of cell adhesion and activation of integrin β1/ERK/VCL pathway. Our ﬁndings may provide new insights in MSCs intra-articular injection for OA treatment.
format	article
author	Junjun Yang Xin Wang Yahan Fan Xiongbo Song Jiangyi Wu Zhenlan Fu Tao Li Yang Huang ZheXiong Tang Shuo Meng Na Liu Jiajia Chen Pingju Liu Liu Yang Xiaoyuan Gong Cheng Chen
author_facet	Junjun Yang Xin Wang Yahan Fan Xiongbo Song Jiangyi Wu Zhenlan Fu Tao Li Yang Huang ZheXiong Tang Shuo Meng Na Liu Jiajia Chen Pingju Liu Liu Yang Xiaoyuan Gong Cheng Chen
author_sort	Junjun Yang
title	Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression
title_short	Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression
title_full	Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression
title_fullStr	Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression
title_full_unstemmed	Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression
title_sort	tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad mscs and reduces osteoarthritis progression
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/fc292ee3ec3a4903a8ed6c324d31e6e7
work_keys_str_mv	AT junjunyang tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT xinwang tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT yahanfan tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT xiongbosong tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT jiangyiwu tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT zhenlanfu tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT taoli tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT yanghuang tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT zhexiongtang tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT shuomeng tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT naliu tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT jiajiachen tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT pingjuliu tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT liuyang tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT xiaoyuangong tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression AT chengchen tropoelastinimprovesadhesionandmigrationofintraarticularinjectedinfrapatellarfatpadmscsandreducesosteoarthritisprogression
_version_	1718408357566480384

Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression

Ejemplares similares