Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential

Abstract Clostridium perfringens causes a plethora of devastating infections, with toxin production being the underlying mechanism of pathogenicity in various hosts. Genomic analyses of 206 public-available C. perfringens strains´ sequence data identified a substantial degree of genomic variability...

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Autores principales:	Mostafa Y. Abdel-Glil, Prasad Thomas, Jörg Linde, Anne Busch, Lothar H. Wieler, Heinrich Neubauer, Christian Seyboldt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc2d098e43f04c4a87fa3ba28dd8bd5e
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spelling	oai:doaj.org-article:fc2d098e43f04c4a87fa3ba28dd8bd5e2021-12-02T11:45:03ZComparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential10.1038/s41598-021-86148-82045-2322https://doaj.org/article/fc2d098e43f04c4a87fa3ba28dd8bd5e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86148-8https://doaj.org/toc/2045-2322Abstract Clostridium perfringens causes a plethora of devastating infections, with toxin production being the underlying mechanism of pathogenicity in various hosts. Genomic analyses of 206 public-available C. perfringens strains´ sequence data identified a substantial degree of genomic variability in respect to episome content, chromosome size and mobile elements. However, the position and order of the local collinear blocks on the chromosome showed a considerable degree of preservation. The strains were divided into five stable phylogroups (I–V). Phylogroup I contained human food poisoning strains with chromosomal enterotoxin (cpe) and a Darmbrand strain characterized by a high frequency of mobile elements, a relatively small genome size and a marked loss of chromosomal genes, including loss of genes encoding virulence traits. These features might correspond to the adaptation of these strains to a particular habitat, causing human foodborne illnesses. This contrasts strains that belong to phylogroup II where the genome size points to the acquisition of genetic material. Most strains of phylogroup II have been isolated from enteric lesions in horses and dogs. Phylogroups III, IV and V are heterogeneous groups containing a variety of different strains, with phylogroup III being the most abundant (65.5%). In conclusion, C. perfringens displays five stable phylogroups reflecting different disease involvements, prompting further studies on the evolution of this highly important pathogen.Mostafa Y. Abdel-GlilPrasad ThomasJörg LindeAnne BuschLothar H. WielerHeinrich NeubauerChristian SeyboldtNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mostafa Y. Abdel-Glil Prasad Thomas Jörg Linde Anne Busch Lothar H. Wieler Heinrich Neubauer Christian Seyboldt Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential
description	Abstract Clostridium perfringens causes a plethora of devastating infections, with toxin production being the underlying mechanism of pathogenicity in various hosts. Genomic analyses of 206 public-available C. perfringens strains´ sequence data identified a substantial degree of genomic variability in respect to episome content, chromosome size and mobile elements. However, the position and order of the local collinear blocks on the chromosome showed a considerable degree of preservation. The strains were divided into five stable phylogroups (I–V). Phylogroup I contained human food poisoning strains with chromosomal enterotoxin (cpe) and a Darmbrand strain characterized by a high frequency of mobile elements, a relatively small genome size and a marked loss of chromosomal genes, including loss of genes encoding virulence traits. These features might correspond to the adaptation of these strains to a particular habitat, causing human foodborne illnesses. This contrasts strains that belong to phylogroup II where the genome size points to the acquisition of genetic material. Most strains of phylogroup II have been isolated from enteric lesions in horses and dogs. Phylogroups III, IV and V are heterogeneous groups containing a variety of different strains, with phylogroup III being the most abundant (65.5%). In conclusion, C. perfringens displays five stable phylogroups reflecting different disease involvements, prompting further studies on the evolution of this highly important pathogen.
format	article
author	Mostafa Y. Abdel-Glil Prasad Thomas Jörg Linde Anne Busch Lothar H. Wieler Heinrich Neubauer Christian Seyboldt
author_facet	Mostafa Y. Abdel-Glil Prasad Thomas Jörg Linde Anne Busch Lothar H. Wieler Heinrich Neubauer Christian Seyboldt
author_sort	Mostafa Y. Abdel-Glil
title	Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential
title_short	Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential
title_full	Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential
title_fullStr	Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential
title_full_unstemmed	Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential
title_sort	comparative in silico genome analysis of clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fc2d098e43f04c4a87fa3ba28dd8bd5e
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Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential

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