Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy

This study developed low-cost and highly sensitive immunoassay devices possessing the ability to rapidly analyze urine samples. Further, they can quantitatively detect three biomarkers indicating renal injury: monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), and liver-type fatty acid b...

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Autores principales:	Toshihiro Kasama, Miaomiao Sun, Noritada Kaji, Shin’ichi Akiyama, Yukio Yuzawa, Manabu Tokeshi, Seiichi Matsuo, Yoshinobu Baba
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	immunoassay microchannel device microbead biomarker diabetic nephropathy urine Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/fc38cb2a25fb487489de213d2d1f4fe2
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id	oai:doaj.org-article:fc38cb2a25fb487489de213d2d1f4fe2
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spelling	oai:doaj.org-article:fc38cb2a25fb487489de213d2d1f4fe22021-11-25T18:23:20ZMicrochip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy10.3390/mi121113532072-666Xhttps://doaj.org/article/fc38cb2a25fb487489de213d2d1f4fe22021-10-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1353https://doaj.org/toc/2072-666XThis study developed low-cost and highly sensitive immunoassay devices possessing the ability to rapidly analyze urine samples. Further, they can quantitatively detect three biomarkers indicating renal injury: monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), and liver-type fatty acid binding protein (L-FABP). The devices were used to successfully estimate the concentrations of the three biomarkers in urine samples within 2 min; the results were consistent with those obtained via conventional enzyme-linked immunosorbent assay (ELISA), which requires several hours. In addition, the estimated detection limits for the three biomarkers were comparable to those of commercially available ELISA kits. Thus, the proposed and fabricated devices facilitate high-precision and frequent monitoring of renal function.Toshihiro KasamaMiaomiao SunNoritada KajiShin’ichi AkiyamaYukio YuzawaManabu TokeshiSeiichi MatsuoYoshinobu BabaMDPI AGarticleimmunoassaymicrochannel devicemicrobeadbiomarkerdiabetic nephropathyurineMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1353, p 1353 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunoassay microchannel device microbead biomarker diabetic nephropathy urine Mechanical engineering and machinery TJ1-1570
spellingShingle	immunoassay microchannel device microbead biomarker diabetic nephropathy urine Mechanical engineering and machinery TJ1-1570 Toshihiro Kasama Miaomiao Sun Noritada Kaji Shin’ichi Akiyama Yukio Yuzawa Manabu Tokeshi Seiichi Matsuo Yoshinobu Baba Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy
description	This study developed low-cost and highly sensitive immunoassay devices possessing the ability to rapidly analyze urine samples. Further, they can quantitatively detect three biomarkers indicating renal injury: monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), and liver-type fatty acid binding protein (L-FABP). The devices were used to successfully estimate the concentrations of the three biomarkers in urine samples within 2 min; the results were consistent with those obtained via conventional enzyme-linked immunosorbent assay (ELISA), which requires several hours. In addition, the estimated detection limits for the three biomarkers were comparable to those of commercially available ELISA kits. Thus, the proposed and fabricated devices facilitate high-precision and frequent monitoring of renal function.
format	article
author	Toshihiro Kasama Miaomiao Sun Noritada Kaji Shin’ichi Akiyama Yukio Yuzawa Manabu Tokeshi Seiichi Matsuo Yoshinobu Baba
author_facet	Toshihiro Kasama Miaomiao Sun Noritada Kaji Shin’ichi Akiyama Yukio Yuzawa Manabu Tokeshi Seiichi Matsuo Yoshinobu Baba
author_sort	Toshihiro Kasama
title	Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy
title_short	Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy
title_full	Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy
title_fullStr	Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy
title_full_unstemmed	Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy
title_sort	microchip immunoassays for monitoring renal function: rapid, low-cost, and highly sensitive quantification of urinary biomarkers of diabetic nephropathy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fc38cb2a25fb487489de213d2d1f4fe2
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Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy

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