A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases
Molina-Cruz et al. analyzed 4,971 sequences of the Plasmodium falciparum Pfs47 gene and identified a combination of two SNP haplotypes highly correlated with geographic origin and transmission potential by mosquito vectors. A simple High-Resolution Melting RT-PCR assay was developed to genotype thes...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/fc42e52755fa4bd685f6c6320aeedb3b |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:fc42e52755fa4bd685f6c6320aeedb3b |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:fc42e52755fa4bd685f6c6320aeedb3b2021-12-02T19:16:53ZA genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases10.1038/s42003-021-02667-02399-3642https://doaj.org/article/fc42e52755fa4bd685f6c6320aeedb3b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02667-0https://doaj.org/toc/2399-3642Molina-Cruz et al. analyzed 4,971 sequences of the Plasmodium falciparum Pfs47 gene and identified a combination of two SNP haplotypes highly correlated with geographic origin and transmission potential by mosquito vectors. A simple High-Resolution Melting RT-PCR assay was developed to genotype these SNPs and establish the likely continent of origin and transmission risk of P. falciparum isolates.Alvaro Molina-CruzNadia RaytselisRoxanne WithersAnkit DwivediPeter D. CromptonBoubacar TraoreGiovanna CarpiJoana C. SilvaCarolina Barillas-MuryNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-6 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Biology (General) QH301-705.5 |
| spellingShingle |
Biology (General) QH301-705.5 Alvaro Molina-Cruz Nadia Raytselis Roxanne Withers Ankit Dwivedi Peter D. Crompton Boubacar Traore Giovanna Carpi Joana C. Silva Carolina Barillas-Mury A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases |
| description |
Molina-Cruz et al. analyzed 4,971 sequences of the Plasmodium falciparum Pfs47 gene and identified a combination of two SNP haplotypes highly correlated with geographic origin and transmission potential by mosquito vectors. A simple High-Resolution Melting RT-PCR assay was developed to genotype these SNPs and establish the likely continent of origin and transmission risk of P. falciparum isolates. |
| format |
article |
| author |
Alvaro Molina-Cruz Nadia Raytselis Roxanne Withers Ankit Dwivedi Peter D. Crompton Boubacar Traore Giovanna Carpi Joana C. Silva Carolina Barillas-Mury |
| author_facet |
Alvaro Molina-Cruz Nadia Raytselis Roxanne Withers Ankit Dwivedi Peter D. Crompton Boubacar Traore Giovanna Carpi Joana C. Silva Carolina Barillas-Mury |
| author_sort |
Alvaro Molina-Cruz |
| title |
A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases |
| title_short |
A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases |
| title_full |
A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases |
| title_fullStr |
A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases |
| title_full_unstemmed |
A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases |
| title_sort |
genotyping assay to determine geographic origin and transmission potential of plasmodium falciparum malaria cases |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/fc42e52755fa4bd685f6c6320aeedb3b |
| work_keys_str_mv |
AT alvaromolinacruz agenotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT nadiaraytselis agenotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT roxannewithers agenotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT ankitdwivedi agenotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT peterdcrompton agenotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT boubacartraore agenotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT giovannacarpi agenotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT joanacsilva agenotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT carolinabarillasmury agenotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT alvaromolinacruz genotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT nadiaraytselis genotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT roxannewithers genotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT ankitdwivedi genotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT peterdcrompton genotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT boubacartraore genotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT giovannacarpi genotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT joanacsilva genotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases AT carolinabarillasmury genotypingassaytodeterminegeographicoriginandtransmissionpotentialofplasmodiumfalciparummalariacases |
| _version_ |
1718376950363324416 |